Recombinant Human Annexin A6 Protein, CF

R&D Systems | Catalog # 5186-A6

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human Annexin A6 Protein (5186-A6)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P08133

Applications

Binding Activity
View all Annexin A6 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Annexin A6 protein
Ala2-Asp673, with a C-terminal 6-His tag

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala2

Predicted Molecular Mass

76.5 kDa

SDS-PAGE

70 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized rhAnnexin-A6 can bind rhDecorin with an apparent KD <2 nM.

Formulation, Preparation, and Storage

5186-A6
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Annexin A6

The Annexins comprise a family of proteins that are involved in many aspects of cellular membrane dynamics and the regulation of membrane-associated proteins. They are characterized by multiple repeats of 60 - 70 amino acids (aa) which assemble into a curved disc-like domain that binds to membrane phospholipids in a calcium-dependent manner (1). Human Annexin A6, also known as Annexin VI, Lipocortin VI, and Chromobindin 20, is a 68 kDa protein that consists of eight tandem annexin repeats (2). Alternate splicing results in the deletion of 6 aa at the start of the seventh repeat (3). Human Annexin A6 shares 95% aa sequence identity with mouse and rat Annexin A6. Within the cell, Annexin A6 is involved in the regulation of membrane vesicle trafficking and chloride and calcium ion conductance (1, 4). Annexin A6 is also secreted into the bile and is present on the cell surface (5 - 7). Annexin A6 binds to chondroitin sulfate in a calcium-dependent manner and to heparan sulfate independently of calcium (6, 8). These interactions enable cell surface Annexin A6 to mediate adhesion such as that between macrophages and hepatic sinusoidal endothelial cells (6, 7).

References

  1. Gerke, V. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:449.
  2. Crompton, M.R. et al. (1988) EMBO J. 7:21.
  3. Smith, P.D. et al. (1994) Proc. Natl. Acad. Sci. 91:2713.
  4. Grewal, T. et al. (2006) Bioessays 28:1211.
  5. Thorin, B. et al. (1995) Biochem. Biophys. Res. Commun. 209:1039.
  6. Takagi, H. et al. (2002) J. Cell Sci. 115:3309.
  7. Tressler, R.J. et al. (1994) Exp. Cell Res. 215:395.
  8. Ishitsuka, R. et al. (1998) J. Biol. Chem. 273:9935.

Alternate Names

ANX6, ANXA6, Calelectrin, CBP68, CPB-II, Lipocortin VI

Entrez Gene IDs

309 (Human); 11749 (Mouse); 79125 (Rat)

Gene Symbol

ANXA6

UniProt

P08133 (Human)

Additional Annexin A6 Products

Product Documents for Recombinant Human Annexin A6 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Annexin A6 Protein, CF

For research use only

Citations for Recombinant Human Annexin A6 Protein, CF

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