ARSB is a lysosomal enzyme of the sulfatase family that functions as an N-acetylgalactosamine-4-sulfatase (1). The enzyme is also active against chondroitin sulfate and dermatan sulfate (2). Deficiencies in ARSB activity are responsible for the lysosomal storage disease mucopolysaccharidosis Type VI, also known as Maroteaux-Lamy Syndrome (3).
Recombinant Human Arylsulfatase B/ARSB Protein, CF
R&D Systems | Catalog # 4415-SU
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Key Product Details
- R&D Systems CHO-derived Recombinant Human Arylsulfatase B/ARSB Protein (4415-SU)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
CHO
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human Arylsulfatase B/ARSB protein
Ser37-Met533, with a C-terminal 10-His tag
Ser37-Met533, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser37
Predicted Molecular Mass
57 kDa
SDS-PAGE
80 kDa, reducing conditions
Activity
Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS).
The specific activity is >3,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >3,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation, and Storage
4415-SU
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Arylsulfatase B/ARSB
References
- Peters, C. et al. (1990) J. Biol. Chem. 265:3374.
- O’Brien, J.S. et al. (1974) Biochem. Biophys. Res. Commun. 60:1170.
- Wicker, G. et al. (1991) J. Biol. Chem. 266:21386.
Alternate Names
ARSB, ASB, N-acetylgalactosamine-4-sulfatase
Gene Symbol
ARSB
UniProt
Additional Arylsulfatase B/ARSB Products
Product Documents for Recombinant Human Arylsulfatase B/ARSB Protein, CF
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Arylsulfatase B/ARSB Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human Arylsulfatase B/ARSB Protein, CF
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Protocols
View specific protocols for Recombinant Human Arylsulfatase B/ARSB Protein, CF (4415-SU):
Materials
- Assay Buffer: 50 mM MES, pH 6.5
- Recombinant Human Arylsulfatase B/ARSB (rhARSB) (Catalog # 4415-SU)
- Substrate: 4-Nitrocatechol Sulfate (4-PNCS) (Sigma, Catalog # N-7251)
- NaOH (Sigma, Catalog # S-0899)
- 96-well Clear Plate (Costar, Catalog # 92592)
- Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
- Dilute rhARSB to 1 µg/mL in Assay Buffer.
- Dilute Substrate to 2 mM in Assay Buffer.
- Combine 75 µL of 1 µg/mL rhARSB and 75 µL of 2 mM Substrate. Include a Substrate Blank containing 75 µL Assay Buffer and 75 µL Substrate.
- Incubate at 37 °C for 1 hour.
- Stop reaction by adding 150 µL of 0.2 M NaOH.
- Load 200 µL of reaction into a plate.
- Read at 510 nm (absorbance) in endpoint mode.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Abs* (OD) x Conversion Factor** (pmol/OD) |
| Incubation time (min) x amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard P-Nitrocatechol (PNC) (Sigma, Catalog # N15553).
Per Well:- rhARSB: 0.050 µg
- Substrate: 0.5 mM