Recombinant Human BAI3 Protein, CF

  
  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human C1qL3 (Catalog # 9115-TN) is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI3 that produces 50% of the optimal binding response is approximately 0.3-1.8 μg/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived Ala25-Thr880, with C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala25
  • Predicted Molecular Mass
    97 kDa
  • SDS-PAGE
    109-132 kDa, reducing conditions
9106-BA
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Recombinant Human C1qL3 (Catlog # 9115-TN) is coated at 1 µg/mL (100 μL/well), Recombinant Human BAI3 (Catlog # 9106-BA) binds with a typical ED50 of 0.3-1.8 μg/mL.
Background: BAI3
Brain angiogenesis inhibitor 3 (BAI3) is an approximately 180 kDa adhesion GPCR that plays a role in neuronal synapse formation and maintenance. Mature human BAI3 consists of an 856 amino acid (aa) N-terminal extracellular domain (ECD) with one CUB domain, four TSP1 domains, and a GPS protease sensitive linker, followed by a region with seven transmembrane segments and a 376 aa C-terminal cytoplasmic domain (1, 2). Within the N-terminal ECD, human BAI3 shares 98% and 97% aa sequence identity with mouse and rat BAI3, respectively. BAI3 is primarily expressed post-synaptically in the cerebral cortex and on cerebellar Purkinje cells (1-5), and it binds to the TNF-like proteins C1qL1, 2, 3, and 4 (6). BAI3 interaction with C1qL1 is required for the formation and maintenance of excitatory synapses between climbing fibers and parallel fibers with Purkinje cells (4, 5). BAI3 ligation can also reduce excitatory synaptic density in hippocampal neurons, decrease dendrite arborization, and promote axon pruning in climbing fibers (3, 4, 6). It is additionally expressed in developing muscle where it is required for myoblast fusion (7).
  • References:
    1. Shiratsuchi, T. et al. (1997) Cytogenet. Cell Genet. 79:103.
    2. Kee, H.J. et al. (2004) FEBS Lett. 569:307.
    3. Lanoue, V. et al. (2013) Mol. Psychiatry 18:943.
    4. Kakegawa, W. et al. (2015) Neuron 85:316.
    5. Sigoillot, S.M. et al. (2015) Cell Rep. 10:820.
    6. Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.
    7. Hamoud, N. et al. (2014) Proc. Natl. Acad. Sci. USA 111:3745.
  • Long Name:
    Brain-specific Angiogenesis Inhibitor 3
  • Entrez Gene IDs:
    577 (Human); 210933 (Mouse); 301309 (Rat)
  • Alternate Names:
    BAI3; brain-specific angiogenesis inhibitor 3; dJ91B17.1 (brain-specific angiogenesis inhibitor 3); KIAA0550MGC133100
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
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