Recombinant Human BAI3 Protein, CF

Catalog # Availability Size / Price Qty
9106-BA-050
Recombinant Human BAI3 Protein, CF Bioactivity
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Recombinant Human BAI3 Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human C1qL3 (Catalog # 9115-TN) is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI3 that produces 50% of the optimal binding response is approximately 0.3-1.8 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human BAI3 protein
Ala25-Thr880, with C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ala25
Predicted Molecular Mass
97 kDa
SDS-PAGE
109-132 kDa, reducing conditions

Product Datasheets

9106-BA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human BAI3 Protein, CF Bioactivity View Larger

When Recombinant Human C1qL3 (Catlog # 9115-TN) is coated at 1 µg/mL (100 μL/well), Recombinant Human BAI3 (Catlog # 9106-BA) binds with a typical ED50 of 0.3-1.8 μg/mL.

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Background: BAI3

Brain angiogenesis inhibitor 3 (BAI3) is an approximately 180 kDa adhesion GPCR that plays a role in neuronal synapse formation and maintenance. Mature human BAI3 consists of an 856 amino acid (aa) N-terminal extracellular domain (ECD) with one CUB domain, four TSP1 domains, and a GPS protease sensitive linker, followed by a region with seven transmembrane segments and a 376 aa C-terminal cytoplasmic domain (1, 2). Within the N-terminal ECD, human BAI3 shares 98% and 97% aa sequence identity with mouse and rat BAI3, respectively. BAI3 is primarily expressed post-synaptically in the cerebral cortex and on cerebellar Purkinje cells (1-5), and it binds to the TNF-like proteins C1qL1, 2, 3, and 4 (6). BAI3 interaction with C1qL1 is required for the formation and maintenance of excitatory synapses between climbing fibers and parallel fibers with Purkinje cells (4, 5). BAI3 ligation can also reduce excitatory synaptic density in hippocampal neurons, decrease dendrite arborization, and promote axon pruning in climbing fibers (3, 4, 6). It is additionally expressed in developing muscle where it is required for myoblast fusion (7).

References
  1. Shiratsuchi, T. et al. (1997) Cytogenet. Cell Genet. 79:103.
  2. Kee, H.J. et al. (2004) FEBS Lett. 569:307.
  3. Lanoue, V. et al. (2013) Mol. Psychiatry 18:943.
  4. Kakegawa, W. et al. (2015) Neuron 85:316.
  5. Sigoillot, S.M. et al. (2015) Cell Rep. 10:820.
  6. Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.
  7. Hamoud, N. et al. (2014) Proc. Natl. Acad. Sci. USA 111:3745.
Long Name
Brain-specific Angiogenesis Inhibitor 3
Entrez Gene IDs
577 (Human); 210933 (Mouse); 301309 (Rat)
Alternate Names
BAI3; brain-specific angiogenesis inhibitor 3; dJ91B17.1 (brain-specific angiogenesis inhibitor 3); KIAA0550MGC133100

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