Recombinant Human BAI3 Protein, CF Summary
Ala25-Thr880, with C-terminal 6-His tag
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 1 mg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human C1qL3 (Catlog # 9115-TN) is coated at 1 µg/mL (100 μL/well), Recombinant Human BAI3 (Catlog # 9106-BA) binds with a typical ED50 of 0.3-1.8 μg/mL.
Brain angiogenesis inhibitor 3 (BAI3) is an approximately 180 kDa adhesion GPCR that plays a role in neuronal synapse formation and maintenance. Mature human BAI3 consists of an 856 amino acid (aa) N-terminal extracellular domain (ECD) with one CUB domain, four TSP1 domains, and a GPS protease sensitive linker, followed by a region with seven transmembrane segments and a 376 aa C-terminal cytoplasmic domain (1, 2). Within the N-terminal ECD, human BAI3 shares 98% and 97% aa sequence identity with mouse and rat BAI3, respectively. BAI3 is primarily expressed post-synaptically in the cerebral cortex and on cerebellar Purkinje cells (1-5), and it binds to the TNF-like proteins C1qL1, 2, 3, and 4 (6). BAI3 interaction with C1qL1 is required for the formation and maintenance of excitatory synapses between climbing fibers and parallel fibers with Purkinje cells (4, 5). BAI3 ligation can also reduce excitatory synaptic density in hippocampal neurons, decrease dendrite arborization, and promote axon pruning in climbing fibers (3, 4, 6). It is additionally expressed in developing muscle where it is required for myoblast fusion (7).
- Shiratsuchi, T. et al. (1997) Cytogenet. Cell Genet. 79:103.
- Kee, H.J. et al. (2004) FEBS Lett. 569:307.
- Lanoue, V. et al. (2013) Mol. Psychiatry 18:943.
- Kakegawa, W. et al. (2015) Neuron 85:316.
- Sigoillot, S.M. et al. (2015) Cell Rep. 10:820.
- Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.
- Hamoud, N. et al. (2014) Proc. Natl. Acad. Sci. USA 111:3745.
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