Recombinant Human Betacellulin Protein

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Carrier Free

Catalog # Availability Size / Price Qty
261-CE-010/CF
261-CE-050/CF
261-CE-250/CF
261-CE-01M/CF

With Carrier

Catalog # Availability Size / Price Qty
261-CE-010
261-CE-050
261-CE-250
Recombinant Human Betacellulin/BTC Protein SEC-MALS.
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Product Details
Citations (11)
FAQs
Reviews

Recombinant Human Betacellulin Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 0.15-0.6 ng/mL.
Source
E. coli-derived human Betacellulin/BTC protein
Asp32-Tyr111
Accession #
N-terminal Sequence
Analysis
Asp32
Predicted Molecular Mass
9 kDa
SDS-PAGE
15 kDa, reducing conditions

Product Datasheets

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261-CE (with carrier)

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261-CE/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

261-CE

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

261-CE/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SEC-MALS View Larger

Recombinant Human Betacellulin (Catalog # 261-CE) has a molecular weight (MW) of 10.3 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.

Reconstitution Calculator

Reconstitution Calculator

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Background: Betacellulin/BTC

Betacellulin (BTC) is a new member of the EGF family of cytokines that is comprised of at least ten proteins including EGF, TGF-alpha, amphiregulin, HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or more EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotein composed of 80 amino acid residues. The cDNA encoding human BTC was cloned from a human breast adenocarcinoma cell line (MCF-7) cDNA library. Human and mouse cDNAs encode BTC precursor proteins of 178 and 177 amino acid residues, respectively. At the amino acid sequence level, human BTC precursor protein exhibits 79% identity with that of the mouse BTC precursor. In a mouse cell line transfected with human BTC cDNA, three forms of soluble human BTC have been detected: the glycosylated, intact BTC composed of 80 amino acid residues, a truncated molecule lacking 12 amino acid residues from the amino terminus, and a second truncated molecule lacking 30 amino acid residues from the amino terminus. The biological activities of these BTC forms were shown to be identical. BTC can bind to the EGF receptor and is a potent mitogen for Balb/c 3T3 fibroblasts, retinal pigment epithelial cells and vascular smooth muscle cells.

Entrez Gene IDs
685 (Human); 12223 (Mouse)
Alternate Names
Betacellulin; BTC; probetacellulin

Citations for Recombinant Human Betacellulin Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Insulin regulates human pancreatic endocrine cell differentiation in vitro
    Authors: Cota, P;Caliskan, ÖS;Bastidas-Ponce, A;Jing, C;Jaki, J;Saber, L;Czarnecki, O;Taskin, D;Blöchinger, AK;Kurth, T;Sterr, M;Burtscher, I;Krahmer, N;Lickert, H;Bakhti, M;
    Molecular metabolism
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Generation of Isogenic hiPSCs with Targeted Edits at Multiple Intronic SNPs to Study the Effects of the Type 2 Diabetes Associated KCNQ1 Locus in American Indians
    Authors: AK Nair, M Traurig, JR Sutherland, YL Muller, ED Grellinger, L Saporito, RG Nelson, C Bogardus, LJ Baier
    Cells, 2022-04-25;11(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells
    Authors: NJ Hogrebe, P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman
    Nat. Biotechnol., 2020-02-24;0(0):.
    Species: Human
    Sample Types: Cells
    Applications: Cell Culture
  4. HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells
    Authors: Y Wang, L Lifshitz, K Gellatly, CL Vinton, K Busman-Sah, S McCauley, P Vangala, K Kim, A Derr, S Jaiswal, A Kucukural, P McDonel, PW Hunt, T Greenough, J Houghton, M Somsouk, JD Estes, JM Brenchley, M Garber, SG Deeks, J Luban
    Nat. Immunol., 2020-02-17;21(3):274-286.
    Species: Human
    Sample Types: Cells
    Applications: Cell Culture
  5. Betacellulin enhances ovarian cancer cell migration by up-regulating Connexin43 via MEK-ERK signaling
    Authors: J Zhao, C Klausen, Y Yi, JC Cheng, HM Chang, PCK Leung
    Cell. Signal., 2019-10-22;65(0):109439.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. MRI tracking of autologous pancreatic progenitor-derived insulin-producing cells in monkeys
    Authors: C Zou, Y Lu, X Teng, S Wang, X Sun, F Huang, G Shu, X Huang, H Guo, Z Chen, J Zhang, YA Zhang
    Sci Rep, 2017-05-31;7(1):2505.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Effects of neuregulin GGF2 (cimaglermin alfa) dose and treatment frequency on left ventricular function in rats following myocardial infarction
    Authors: Tom J Parry
    Eur. J. Pharmacol, 2016-12-16;796(0):76-89.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  8. Glial growth factor 2 promotes functional recovery with treatment initiated up to 7 days after permanent focal ischemic stroke.
    Authors: Iaci JF, Ganguly A, Finklestein SP, Parry TJ, Ren J, Saha S, Sietsma DK, Srinivas M, Vecchione AM, Caggiano AO
    Neuropharmacology, 2010-08-04;59(7):640-9.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  9. Remission of diabetes by beta-cell regeneration in diabetic mice treated with a recombinant adenovirus expressing betacellulin.
    Authors: Shin S, Li N, Kobayashi N, Yoon JW, Jun HS
    Mol. Ther., 2008-03-18;16(5):854-61.
    Applications: ELISA (Standard)
  10. Hydrogen peroxide and endothelin-1 are novel activators of betacellulin ectodomain shedding.
    Authors: Sanderson MP, Abbott CA, Tada H, Seno M, Dempsey PJ, Dunbar AJ
    J. Cell. Biochem., 2006-10-01;99(2):609-23.
    Applications: ELISA (Standard)
  11. Development of an in vitro pancreatic tissue model to study regulation of islet neogenesis associated protein expression.
    Authors: Petropavlovskaia M, Makhlin J, Sampalis J, Rosenberg L
    J. Endocrinol., 2006-10-01;191(1):65-81.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Bioassay

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