Recombinant Human Biliverdin Reductase A/BLVRA Protein, CF

R&D Systems | Catalog # 6454-BR

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems E. coli-derived Recombinant Human Biliverdin Reductase A/BLVRA Protein (6454-BR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P53004

Applications

Enzyme Activity
View all Biliverdin Reductase A/BLVRA Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Biliverdin Reductase A/BLVRA protein
Glu6-Ser294, with an N-terminal Met and C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

34 kDa

SDS-PAGE

38 kDa, reducing conditions

Activity

Measured by the reduction of biliverdin IX alpha to bilirubin.
The specific activity is >450 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation, and Storage

6454-BR
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, Brij and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Biliverdin Reductase A/BLVRA

The clearance of heme in mammals is a two-step process starting with the conversion of heme to biliverdin by heme oxygenase, followed by reduction of biliverdin to bilirubin by biliveredin reductase. Biliverdin Reductase A (BLVRA) converts the alpha  isomer of biliverdin IX, which constitutes 95‑97% of total biliverdin isomers in adults, to bilirubin IX-alpha (1). Although bilirubin is known to be a toxic pigment that needs to be excreted, it is also a physiological antioxidant (2). Therefore BLVRA enables continuous protection of cells against oxidative stress. BLVRA is a cytosolic protein that is highly expressed in the liver, but is also present in most tissues. The two N‑terminal residues are a pro-sequence that is missing in the mature protein (3). The reduction of biliverdin by BLVRA is coupled to oxidation of pyridine nucleotide co‑factors NADH and NADPH with distinct pH optima, 6.7 for NADH and 8.7 for NADPH (4). BLVRA has many functions independent of its reductase activity. It is also a dual-specificity (Ser/Thr + Tyr) protein kinase (5) and an activator of protein kinase C bII (6) and ERK (7). It functions as a transcription factor regulating heme oxygenase gene expression (8).

References

  1. Yamaguchi, T. et al. (1994) J. Biol. Chem. 269:24343.
  2. Stocker, R. et al. (1987) Science 235:1043.
  3. Fakhrai, H. and M.D. Maines. (1992) J. Biol. Chem. 267:4023.
  4. Maines, M. D. et al. (1996) Eur. J. Biochem. 235:372.
  5. Kapitulnik, J. and M.D. Maines. (2009) Trends Pharmacol. Sci. 30:129.
  6. Maines, M.D. et al. (2007) J. Biol. Chem. 282:8110.
  7. Lerner-Marmarosh, N. et al. (2008) Proc. Natl. Acad. Sci. USA. 105:6870.
  8. Tudor, C. et al. (2008) Biochem. J. 413:405.

Alternate Names

BLVRA, BVR, BVRA

Entrez Gene IDs

644 (Human)

Gene Symbol

BLVRA

UniProt

P53004

Additional Biliverdin Reductase A/BLVRA Products

Product Documents for Recombinant Human Biliverdin Reductase A/BLVRA Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Biliverdin Reductase A/BLVRA Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

For research use only

Related Research Areas

Redox Enzymes

Customer Reviews for Recombinant Human Biliverdin Reductase A/BLVRA Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Biliverdin Reductase A/BLVRA Protein, CF and earn rewards!

Have you used Recombinant Human Biliverdin Reductase A/BLVRA Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

View specific protocols for Recombinant Human Biliverdin Reductase A/BLVRA Protein, CF (6454-BR):

Materials
  • Assay Buffer: 100 mM Tris, pH 8.5
  • Recombinant Human Biliverdin Reductase A/BLVRA (rhBLVRA) (Catalog # 6454-BR)
  • Bovine Serum Albumin (BSA), 100 mg/mL in deionized water
  • Biliverdin (Frontier Scientific, Catalog # B655-9), 1 mM in DMSO
  • beta -NADPH (Sigma, Catalog # N7505), 10 mM in deionized water
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rhBLVRA to 3 ng/μL in Assay Buffer.
  2. Dilute BSA to 3 mg/mL in Assay Buffer.
  3. Dilute Biliverdin to 60 μM in Assay Buffer.
  4. Combine equal volumes of diluted rhBLVRA, BSA, and Biliverdin to form the reaction mixture. As a control, use Assay Buffer in place of rhBLVRA.
  5. Dilute beta -NADPH to 200 μM in Assay Buffer.
  6. Load 50 μL of reaction mixture and control into the microplate and start the reaction by adding 50 μL of 200 μM beta -NADPH.
  7. Read at an absorbance of 468 nm in kinetic mode for 5 minutes.
  8. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (OD/min) x well volume (L) x 1012 pmol/mol
ext. coeff** (M-1cm-1) x path corr.*** (cm) x amount of enzyme (µg)

     *Adjusted for Control 
     **Using the extinction coefficient 55000 M-1cm-1 
     ***Using the path correction 0.32 cm
     Note: the output of many spectrophotometers is in mOD Per Well:
  • rhBLVRA: 0.05 μg
  • Biliverdin: 10 μM
  • beta -NADPH: 100 μM

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes