Recombinant Human BMP-2/BMP-7 Heterodimer Protein, CF

Formulations:
Catalog # Availability Size / Price Qty
3229-BM-010/CF
Product Details
Citations (14)
FAQs
Reviews

Recombinant Human BMP-2/BMP-7 Heterodimer Protein, CF Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 10-40 ng/mL.
Source
E. coli-derived human BMP-2/BMP-7 Heterodimer protein
Human BMP-2
(Ala284 – Arg396)
Accession # NP_001191
Human BMP-7
(Ser293 – His431)
Accession # NP_001710
N-terminus C-terminus
N-terminal Sequence
Analysis
Ala284 & Ser293
Structure / Form
Disulfide-linked heterodimer
Predicted Molecular Mass
12.9 kDa (BMP-2), 15.8 kDa (BMP-7)

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3229-BM/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3229-BM

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-2/BMP-7 Heterodimer

Human BMP-7, also known as osteogenic protein 1 (OP-1), and BMP-2 are members of the BMP subgroup of the TGF-beta superfamily and signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-2 and BMP-7 influence a variety of morphogenic processes, particularly during skeletal and renal development (1 - 3). The human BMP-2 cDNA encodes a 396 amino acid (aa) precursor that contains a 23 aa signal sequence, a 259 propeptide, and a 114 aa mature protein (4). The human BMP-7 cDNA encodes a 431 aa precursor that contains a 29 aa signal sequence, a 263 aa propeptide, and a 139 aa mature protein (5). BMP propeptides are removed by proteolysis, enabling mature BMPs to form active disulfide linked homodimers or heterodimers (1). Human and mouse BMP-2 and BMP-7 are 100% and 98% identical, respectively, at the amino acid level. Human BMP-2 shares 85% aa sequence identity with human BMP-4 and less than 51% aa sequence identity with other BMPs. Human BMP-7 shares approximately 60% - 70% aa sequence identity with BMP-5, -6, and -8, and less than 50% aa sequence identity with other BMPs. BMP-2 and BMP-7 are co-expressed in some embryonic tissues (6, 7) and associate into a functional 38 kDa osteogenic dimer (8). In in vitro osteoblast differentiation assays and in vivo bone formation models, a BMP-2/BMP-7 heterodimer is significantly more potent than either homodimer (9 - 12). Considering that BMP-2 preferentially binds BMPRIA/ALK-3 and BMPRIB/ALK-6, while BMP-7 is selective for ALK-2, the observed increase in heterodimer activity may be due to the triggering of additional receptor subtypes.

References
  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
  3. Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299.
  4. Wozney, J. et al. (1988) Science 242:1528.
  5. Gregory, K.E. et al. (2005) J. Biol. Chem. 280:27970.
  6. Lyons, K.M. et al. (1995) Mech. Dev. 50:71.
  7. Knosp, W.M. et al. (2004) Development 131:4581.
  8. Sampath, T.K. et al. (1990) J. Biol. Chem. 265:13198.
  9. Hazama, M. et al. (1995) Biochem. Biophys. Res. Commun. 209:859.
  10. Zhu, W. et al. (2004) J. Bone Miner. Res. 19:2021.
  11. Zhao, M. et al. (2005) J. Cell. Biochem. 95:1.
  12. Israel, D.I. et al. (1996) Growth Factors 13:291.
Long Name
Bone Morphogenetic Protein 2/7
Entrez Gene IDs
650 (Human)
Alternate Names
BMP-2/BMP-7 Heterodimer

Citations for Recombinant Human BMP-2/BMP-7 Heterodimer Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation
    Authors: JM Kim, YS Yang, KH Park, X Ge, R Xu, N Li, M Song, H Chun, S Bok, JF Charles, O Filhol-Coc, B Boldyreff, T Dinter, PB Yu, N Kon, W Gu, T Takarada, MB Greenblatt, JH Shim
    Nat Commun, 2020;11(1):2289.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. BLNCR is a long non-coding RNA adjacent to integrin beta-1 that is rapidly lost during epidermal progenitor cell differentiation
    Authors: SEJ Tanis, ES Köksal, JAGL van Buggen, KW Mulder
    Sci Rep, 2019;9(1):31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Feather arrays are patterned by interacting signalling and cell density waves
    Authors: WKW Ho, L Freem, D Zhao, KJ Painter, TE Woolley, EA Gaffney, MJ McGrew, A Tzika, MC Milinkovit, P Schneider, A Drusko, F Matthäus, JD Glover, KL Wells, JA Johansson, MG Davey, HM Sang, M Clinton, DJ Headon
    PLoS Biol., 2019;17(2):e3000132.
    Species: Chicken
    Sample Types: Tissue Explants
    Applications: Bioassay
  4. HACE1 is a potential tumor suppressor in osteosarcoma
    Authors: AM El-Naggar, PW Clarkson, GL Negri, B Turgu, F Zhang, MS Anglesio, PH Sorensen
    Cell Death Dis, 2019;10(1):21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. All-trans retinoic-acid inhibits heterodimeric bone morphogenetic protein 2/7-stimulated osteoclastogenesis, and resorption activity
    Authors: W Bi, Y Liu, J Guo, Z Lin, J Liu, M Zhou, D Wismeijer, JL Pathak, G Wu
    Cell Biosci, 2018;8(0):48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Bone formation by heterodimers through non-viral gene delivery of BMP-2/6 and BMP-2/7
    Authors: LD Loozen, A Vanderstee, AH Kragten, FC Öner, WJ Dhert, MC Kruyt, J Alblas
    Eur Cell Mater, 2018;35(0):195-208.
    Applications: ELISA (Standard)
  7. Splicing and Chromatin Factors Jointly Regulate Epidermal Differentiation
    Authors: SEJ Tanis, PWTC Jansen, H Zhou, SJ van Heerin, M Vermeulen, M Kretz, KW Mulder
    Cell Rep, 2018;25(5):1292-1303.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Directing Differentiation of Pluripotent Stem Cells Toward Retinal Pigment Epithelium Lineage
    Authors: P Choudhary, H Booth, A Gutteridge, B Surmacz, I Louca, J Steer, J Kerby, PJ Whiting
    Stem Cells Transl Med, 2017;6(2):490-501.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. p38alpha MAPK is required for tooth morphogenesis and enamel secretion.
    Authors: Greenblatt M, Kim J, Oh H, Park K, Choo M, Sano Y, Tye C, Skobe Z, Davis R, Park J, Bei M, Glimcher L, Shim J
    J Biol Chem, 2015;290(1):284-95.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  10. A novel strategy for enrichment and isolation of osteoprogenitor cells from induced pluripotent stem cells based on surface marker combination.
    Authors: Ochiai-Shino H, Kato H, Sawada T, Onodera S, Saito A, Takato T, Shibahara T, Muramatsu T, Azuma T
    PLoS ONE, 2014;9(6):e99534.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. FRMD4A upregulation in human squamous cell carcinoma promotes tumor growth and metastasis and is associated with poor prognosis.
    Authors: Goldie S, Mulder K, Tan D, Lyons S, Sims A, Watt F
    Cancer Res, 2012;72(13):3424-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
    Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
    J. Biol. Chem., 2011;286(34):30034-46.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  13. Bone morphogenetic protein heterodimers assemble heteromeric type I receptor complexes to pattern the dorsoventral axis.
    Authors: Little SC, Mullins MC
    Nat. Cell Biol., 2009;11(5):637-43.
    Species: Zebrafish
    Sample Types: Whole Tissue
    Applications: Bioassay
  14. Tailbud-derived Bmp4 drives proliferation and inhibits maturation of zebrafish chordamesoderm.
    Authors: Esterberg R, Delalande JM, Fritz A
    Development, 2008;135(23):3891-901.
    Species: Zebrafish
    Sample Types: In Vivo
    Applications: In Vivo

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