Recombinant Human BMPR-IB/ALK-6 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
505-PR-100

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Recombinant Human BMPR-IB/ALK-6 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit rhBMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.5‑1.5 µg/mL in the presence of 30 ng/mL of rhBMP-4.
Source
Mouse myeloma cell line, NS0-derived human BMPR-IB/ALK-6 protein
Met Human BMPR-IB
(Lys14 - Arg126)
Accession # O00238
IEGRMD Human IgG1
(Pro100 - Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
40 kDa (monomer)
SDS-PAGE
50-55 kDa, reducing conditions

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505-PR

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

505-PR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: BMPR-IB/ALK-6

Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the formation of hetero-oligomeric complexes of the type I and type II serine/threonine kinase receptors. BMP receptor IB (BMPR-IB), also known as activin receptor-like kinase (ALK)-6, is a one of seven known type I serine/threonine kinases that are required for the signal transduction of TGF-beta family cytokines. In contrast to the TGF-beta receptor system in which the type I receptor does not bind TGF-beta  in the absence of the type II receptor, type I receptors involved in BMP signaling (including BMPR-IA, BMPR-IB/ALK-6, and ActR-I/ALK-2) can independently bind the various BMP family proteins in the absence of type II receptors. Recombinant soluble BMPR-IB binds BMP-4 with high-affinity in solution and is a potent BMP-4 antagonist in vitro. BMPR-IB is expressed in various tissues during embryogenesis. In adult tissues, BMPR-IB is only found in the brain. The extracellular domain of BMPR-IB shares little amino acid sequence identity with the other mammalian ALK type I receptor kinases, but the cysteine residues are conserved. Human and mouse BMPR-IB are highly conserved and share 98% sequence identity.

References
  1. Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
  2. Ebendal, T. et al. (1998) J. Neuroscience Research 51:139.
Long Name
Bone Morphogenetic Protein Receptor IB
Entrez Gene IDs
658 (Human); 12167 (Mouse)
Alternate Names
activin receptor-like kinase 6; ALK-6; ALK6CDw293; BMP type-1B receptor; BMPR1B; BMPR-1B; BMPRIB; BMPR-IB; bone morphogenetic protein receptor type-1B; bone morphogenetic protein receptor, type IB; CDw293 antigen; CDw293; EC 2.7.11; EC 2.7.11.30; serine/threonine receptor kinase

Citations for Recombinant Human BMPR-IB/ALK-6 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Facilitated receptor-recognition and enhanced bioactivity of bone morphogenetic protein-2 on magnesium-substituted hydroxyapatite surface
    Authors: B Huang, Y Yuan, T Li, S Ding, W Zhang, Y Gu, C Liu
    Sci Rep, 2016;6(0):24323.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
    Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
    J. Biol. Chem., 2011;286(34):30034-46.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  3. The BMP-binding protein Crossveinless 2 is a short-range, concentration-dependent, biphasic modulator of BMP signaling in Drosophila.
    Authors: Serpe M, Umulis D, Ralston A, Chen J, Olson DJ, Avanesov A, Othmer H, O'Connor MB, Blair SS
    Dev. Cell, 2008;14(6):940-53.
    Species: Drosophila
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  4. A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7.
    Authors: Sengle G, Ono RN, Lyons KM, Bachinger HP, Sakai LY
    J. Mol. Biol., 2008;381(4):1025-39.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA (Detection)
  5. BMP4 regulation of human megakaryocytic differentiation is involved in thrombopoietin signaling.
    Authors: Jeanpierre S, Nicolini FE, Kaniewski B, Dumontet C, Rimokh R, Puisieux A, Maguer-Satta V
    Blood, 2008;112(8):3154-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Crossveinless-2 Is a BMP feedback inhibitor that binds Chordin/BMP to regulate Xenopus embryonic patterning.
    Authors: Ambrosio AL, Taelman VF, Lee HX, Metzinger CA, Coffinier C, De Robertis EM
    Dev. Cell, 2008;15(2):248-60.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  7. Regulation of ADMP and BMP2/4/7 at opposite embryonic poles generates a self-regulating morphogenetic field.
    Authors: Reversade B, De Robertis EM
    Cell, 2005;123(6):1147-60.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Receptor Binding Assay
  8. Specification of dorsal telencephalic character by sequential Wnt and FGF signaling.
    Authors: Gunhaga L, Marklund M, Sjodal M, Hsieh JC, Jessell TM, Edlund T
    Nat. Neurosci., 2003;6(7):701-7.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: Bioassay

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