Recombinant Human BMPR-IB/ALK-6 Fc Chimera Protein, CF

  (8 citations)     
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Citations (8)
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  • Purity
    >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit rhBMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.5‑1.5 µg/mL in the presence of 30 ng/mL of rhBMP-4.
  • Source
    Mouse myeloma cell line, NS0-derived
    Met Human BMPR-IB
    (Lys14 - Arg126)
    Accession # O00238
    IEGRMD Human IgG1
    (Pro100 - Lys330)
    6-His tag
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    40 kDa (monomer)
  • SDS-PAGE
    50-55 kDa, reducing conditions
Product Datasheets

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505-PR
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMPR-IB/ALK-6

Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the formation of hetero-oligomeric complexes of the type I and type II serine/threonine kinase receptors. BMP receptor IB (BMPR-IB), also known as activin receptor-like kinase (ALK)-6, is a one of seven known type I serine/threonine kinases that are required for the signal transduction of TGF-beta family cytokines. In contrast to the TGF-beta receptor system in which the type I receptor does not bind TGF-beta  in the absence of the type II receptor, type I receptors involved in BMP signaling (including BMPR-IA, BMPR-IB/ALK-6, and ActR-I/ALK-2) can independently bind the various BMP family proteins in the absence of type II receptors. Recombinant soluble BMPR-IB binds BMP-4 with high-affinity in solution and is a potent BMP-4 antagonist in vitro. BMPR-IB is expressed in various tissues during embryogenesis. In adult tissues, BMPR-IB is only found in the brain. The extracellular domain of BMPR-IB shares little amino acid sequence identity with the other mammalian ALK type I receptor kinases, but the cysteine residues are conserved. Human and mouse BMPR-IB are highly conserved and share 98% sequence identity.

  • References:
    1. Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
    2. Ebendal, T. et al. (1998) J. Neuroscience Research 51:139.
  • Long Name:
    Bone Morphogenetic Protein Receptor IB
  • Entrez Gene IDs:
    658 (Human); 12167 (Mouse)
  • Alternate Names:
    activin receptor-like kinase 6; ALK-6; ALK6CDw293; BMP type-1B receptor; BMPR1B; BMPR-1B; BMPRIB; BMPR-IB; bone morphogenetic protein receptor type-1B; bone morphogenetic protein receptor, type IB; CDw293 antigen; CDw293; EC 2.7.11; EC 2.7.11.30; serine/threonine receptor kinase
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Facilitated receptor-recognition and enhanced bioactivity of bone morphogenetic protein-2 on magnesium-substituted hydroxyapatite surface
    Authors: B Huang, Y Yuan, T Li, S Ding, W Zhang, Y Gu, C Liu
    Sci Rep, 2016;6(0):24323.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
    Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
    J. Biol. Chem., 2011;286(34):30034-46.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Surface Plasmon Resonance
  3. Crossveinless-2 Is a BMP feedback inhibitor that binds Chordin/BMP to regulate Xenopus embryonic patterning.
    Authors: Ambrosio AL, Taelman VF, Lee HX, Metzinger CA, Coffinier C, De Robertis EM
    Dev. Cell, 2008;15(2):248-60.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Binding Assay
  4. The BMP-binding protein Crossveinless 2 is a short-range, concentration-dependent, biphasic modulator of BMP signaling in Drosophila.
    Authors: Serpe M, Umulis D, Ralston A, Chen J, Olson DJ, Avanesov A, Othmer H, O'Connor MB, Blair SS
    Dev. Cell, 2008;14(6):940-53.
    Species: Drosophila
    Sample Type: Recombinant Protein
    Application: Binding Assay
  5. A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7.
    Authors: Sengle G, Ono RN, Lyons KM, Bachinger HP, Sakai LY
    J. Mol. Biol., 2008;381(4):1025-39.
    Species: Human
    Sample Type: Recombinant Protein
    Application: ELISA Detection
  6. BMP4 regulation of human megakaryocytic differentiation is involved in thrombopoietin signaling.
    Authors: Jeanpierre S, Nicolini FE, Kaniewski B, Dumontet C, Rimokh R, Puisieux A, Maguer-Satta V
    Blood, 2008;112(8):3154-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Regulation of ADMP and BMP2/4/7 at opposite embryonic poles generates a self-regulating morphogenetic field.
    Authors: Reversade B, De Robertis EM
    Cell, 2005;123(6):1147-60.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Receptor Binding Assay
  8. Specification of dorsal telencephalic character by sequential Wnt and FGF signaling.
    Authors: Gunhaga L, Marklund M, Sjodal M, Hsieh JC, Jessell TM, Edlund T
    Nat. Neurosci., 2003;6(7):701-7.
    Species: Chicken
    Sample Type: Whole Tissue
    Application: Bioassay

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Primary Antibodies
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His Tag Antibody

WB , Simple Western , Flow , AP , CyTOF-ready MAB050 26  
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WB , Simple Western MAB050H 3  
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ICFlow IC050P 2  
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His Tag Antibody

WB , Flow , AP MAB050R  
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ICFlow IC050G  
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ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Histidine-tagged Protein Purification Resin

IP999 1
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Recombinant Proteins
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Recombinant Human IgG1 Fc, CF

BA 110-HG 66
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