Recombinant Human BPI Fc Chimera Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human BPI Fc Chimera Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit LPS-induced IL-8 secretion by THP-1 human acute monocytic leukemia cells. Wilde, C.G. et al. (1994) J. Biol. Chem. 269:17411. The ED50 for this effect is 0.4-2.4 ng/mL.
Human embryonic kidney cell, HEK293-derived human BPI protein
Human BPI
Accession # AAA51841
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
77.3 kDa (monomer)
75-85 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: BPI

Bactericidal/Permeability Increasing protein (BPI) is a 55 kDa antibacterial glycoprotein that plays a role in innate immunity (1, 2). It belongs to the lipid transfer protein family that also includes LPS binding protein (LBP), cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). Circulating levels of BPI are positively correlated with the levels of cholesterol, LDL cholesterol, and HDL cholesterol (3). Mature human BPI shares approximately 55% amino acid (aa) sequence identity with mouse and rat BPI. It can be seceted as a monomer or as a disulfide‑linked homodimer (4). It consists of a highly basic N‑terminal and a hydrophobic C‑terminal domain (5). Its N‑terminal domain confers the ability of BPI to bind bacterial lipopolysaccharide (LPS) found in the cell walls of Gram negative bacteria and to induce the lysis and phagocytosis of these bacteria (6‑9). It also blocks the endothelial cell response to endotoxin (10). BPI is stored in neutrophil and eosinophil granules for induced secretion during inflammation (11, 12). It is additionally expressed in mucosal epithelia and testis (10, 13). BPI can be retained on the surface of both neutrophils and epithelial cells, presumably by its hydrophobic C‑terminal domain (8, 10). BPI also functions as an anti-angiogenic molecule by inhibiting vascular endothelial cell proliferation and tubule formation (14). Like the antibacterial actions, this function is mediated by the N‑terminal region (15).

  1. Schultz, H. and J.P. Weiss (2007) Clin. Chim. Acta 384:12.
  2. Holweg, A. et al. (2011) Biochem. Soc. Trans. 39:1045.
  3. Esteve, E. et al. (2010) Thromb. Haemost. 103:780.
  4. Horwitz, A.H. et al. (1996) Protein Exp. Purif. 8:28.
  5. Gray, P.W. et al. (1989) J. Biol. Chem. 264:9505.
  6. Ooi, C.E. et al. (1987) J. Biol. Chem. 262:14891.
  7. Tobias, P.S. et al. (1997) J. Biol. Chem. 272:18682.
  8. Weersink, A.J. et al. (1993) J. Immunol. 150:253.
  9. Nishimura, H. et al. (2001) Immunology 103:519.
  10. Canny, G. et al. (2002) Proc. Natl. Acad. Sci. 99:3902.
  11. Weiss, J. and I. Olsson (1987) Blood 69:652.
  12. Calafat, J. et al. (1998) Blood 91:4770.
  13. Lennartsson, A. et al. (2005) J. Leukoc. Biol. 77:369.
  14. van der Schaft, D.W.J. et al. (2000) Blood 96:176.
  15. Rauniyar, R.K. et al. (2002) Invest. Ophthalmol. Vis. Sci. 43:503.
Long Name
Bactericidal/Permeability-increasing Protein
Entrez Gene IDs
671 (Human); 329547 (Mouse); 296321 (Rat)
Alternate Names
bactericidal permeability-increasing protein; bactericidal/permeability-increasing protein; BPI; BPIFD1; CAP 57; rBPI

Citations for Recombinant Human BPI Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The Yersinia pestis GTPase BipA Promotes Pathogenesis of Primary Pneumonic Plague
    Authors: SD Crane, SK Banerjee, KR Eichelberg, RC Kurten, WE Goldman, RD Pechous
    Infection and Immunity, 2020;0(0):.
    Species: Yersinia pestis
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Recombinant human plasma phospholipid transfer protein (PLTP) to prevent bacterial growth and to treat sepsis
    Authors: V Deckert, S Lemaire, PJ Ripoll, JP de Barros, J Labbé, CC Borgne, V Turquois, G Maquart, D Larose, N Desroche, F Ménétrier, N Le Guern, LJ Lebrun, C Desrumaux, T Gautier, J Grober, C Thomas, D Masson, LM Houdebine, L Lagrost
    Sci Rep, 2017;7(1):3053.
    Species: Bacteria - E. Coli
    Sample Types: In Vivo
    Applications: In Vivo


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