Recombinant Human BPI Fc Chimera Protein, CF Summary
Accession # AAA51841
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Bactericidal/Permeability Increasing protein (BPI) is a 55 kDa antibacterial glycoprotein that plays a role in innate immunity (1, 2). It belongs to the lipid transfer protein family that also includes LPS binding protein (LBP), cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). Circulating levels of BPI are positively correlated with the levels of cholesterol, LDL cholesterol, and HDL cholesterol (3). Mature human BPI shares approximately 55% amino acid (aa) sequence identity with mouse and rat BPI. It can be seceted as a monomer or as a disulfide‑linked homodimer (4). It consists of a highly basic N‑terminal and a hydrophobic C‑terminal domain (5). Its N‑terminal domain confers the ability of BPI to bind bacterial lipopolysaccharide (LPS) found in the cell walls of Gram negative bacteria and to induce the lysis and phagocytosis of these bacteria (6‑9). It also blocks the endothelial cell response to endotoxin (10). BPI is stored in neutrophil and eosinophil granules for induced secretion during inflammation (11, 12). It is additionally expressed in mucosal epithelia and testis (10, 13). BPI can be retained on the surface of both neutrophils and epithelial cells, presumably by its hydrophobic C‑terminal domain (8, 10). BPI also functions as an anti-angiogenic molecule by inhibiting vascular endothelial cell proliferation and tubule formation (14). Like the antibacterial actions, this function is mediated by the N‑terminal region (15).
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- Holweg, A. et al. (2011) Biochem. Soc. Trans. 39:1045.
- Esteve, E. et al. (2010) Thromb. Haemost. 103:780.
- Horwitz, A.H. et al. (1996) Protein Exp. Purif. 8:28.
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- Tobias, P.S. et al. (1997) J. Biol. Chem. 272:18682.
- Weersink, A.J. et al. (1993) J. Immunol. 150:253.
- Nishimura, H. et al. (2001) Immunology 103:519.
- Canny, G. et al. (2002) Proc. Natl. Acad. Sci. 99:3902.
- Weiss, J. and I. Olsson (1987) Blood 69:652.
- Calafat, J. et al. (1998) Blood 91:4770.
- Lennartsson, A. et al. (2005) J. Leukoc. Biol. 77:369.
- van der Schaft, D.W.J. et al. (2000) Blood 96:176.
- Rauniyar, R.K. et al. (2002) Invest. Ophthalmol. Vis. Sci. 43:503.
Citations for Recombinant Human BPI Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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The Yersinia pestis GTPase BipA Promotes Pathogenesis of Primary Pneumonic Plague
Authors: SD Crane, SK Banerjee, KR Eichelberg, RC Kurten, WE Goldman, RD Pechous
Infection and Immunity, 2020;0(0):.
Species: Yersinia pestis
Sample Types: Whole Cells
Applications: Cell Culture
Recombinant human plasma phospholipid transfer protein (PLTP) to prevent bacterial growth and to treat sepsis
Authors: V Deckert, S Lemaire, PJ Ripoll, JP de Barros, J Labbé, CC Borgne, V Turquois, G Maquart, D Larose, N Desroche, F Ménétrier, N Le Guern, LJ Lebrun, C Desrumaux, T Gautier, J Grober, C Thomas, D Masson, LM Houdebine, L Lagrost
Sci Rep, 2017;7(1):3053.
Species: Bacteria - E. Coli
Sample Types: In Vivo
Applications: In Vivo
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