Recombinant Human BPI Fc Chimera Protein, CF

R&D Systems | Catalog # 7468-BP

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human BPI Fc Chimera Protein (7468-BP)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

AAA51841

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all BPI Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human BPI protein
Human BPI
(Val32-Lys487)
Accession # AAA51841		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val32

Predicted Molecular Mass

77.3 kDa (monomer)

SDS-PAGE

75-85 kDa, reducing conditions

Activity

Measured by its ability to inhibit LPS-induced IL-8 secretion by THP-1 human acute monocytic leukemia cells. Wilde, C.G. et al. (1994) J. Biol. Chem. 269:17411.
The ED50 for this effect is 0.4-2.4 ng/mL.

Formulation, Preparation, and Storage

7468-BP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BPI

Bactericidal/Permeability Increasing protein (BPI) is a 55 kDa antibacterial glycoprotein that plays a role in innate immunity (1, 2). It belongs to the lipid transfer protein family that also includes LPS binding protein (LBP), cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). Circulating levels of BPI are positively correlated with the levels of cholesterol, LDL cholesterol, and HDL cholesterol (3). Mature human BPI shares approximately 55% amino acid (aa) sequence identity with mouse and rat BPI. It can be seceted as a monomer or as a disulfide‑linked homodimer (4). It consists of a highly basic N‑terminal and a hydrophobic C‑terminal domain (5). Its N‑terminal domain confers the ability of BPI to bind bacterial lipopolysaccharide (LPS) found in the cell walls of Gram negative bacteria and to induce the lysis and phagocytosis of these bacteria (6‑9). It also blocks the endothelial cell response to endotoxin (10). BPI is stored in neutrophil and eosinophil granules for induced secretion during inflammation (11, 12). It is additionally expressed in mucosal epithelia and testis (10, 13). BPI can be retained on the surface of both neutrophils and epithelial cells, presumably by its hydrophobic C‑terminal domain (8, 10). BPI also functions as an anti-angiogenic molecule by inhibiting vascular endothelial cell proliferation and tubule formation (14). Like the antibacterial actions, this function is mediated by the N‑terminal region (15).

References

  1. Schultz, H. and J.P. Weiss (2007) Clin. Chim. Acta 384:12.
  2. Holweg, A. et al. (2011) Biochem. Soc. Trans. 39:1045.
  3. Esteve, E. et al. (2010) Thromb. Haemost. 103:780.
  4. Horwitz, A.H. et al. (1996) Protein Exp. Purif. 8:28.
  5. Gray, P.W. et al. (1989) J. Biol. Chem. 264:9505.
  6. Ooi, C.E. et al. (1987) J. Biol. Chem. 262:14891.
  7. Tobias, P.S. et al. (1997) J. Biol. Chem. 272:18682.
  8. Weersink, A.J. et al. (1993) J. Immunol. 150:253.
  9. Nishimura, H. et al. (2001) Immunology 103:519.
  10. Canny, G. et al. (2002) Proc. Natl. Acad. Sci. 99:3902.
  11. Weiss, J. and I. Olsson (1987) Blood 69:652.
  12. Calafat, J. et al. (1998) Blood 91:4770.
  13. Lennartsson, A. et al. (2005) J. Leukoc. Biol. 77:369.
  14. van der Schaft, D.W.J. et al. (2000) Blood 96:176.
  15. Rauniyar, R.K. et al. (2002) Invest. Ophthalmol. Vis. Sci. 43:503.

Long Name

Bactericidal/Permeability-increasing Protein

Alternate Names

BPIFD1, CAP 57, rBPI

Entrez Gene IDs

671 (Human); 329547 (Mouse); 296321 (Rat)

Gene Symbol

BPI

UniProt

AAA51841

Additional BPI Products

Product Documents for Recombinant Human BPI Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human BPI Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human BPI Fc Chimera Protein, CF

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