Recombinant Human Cadherin-9 Fc Chimera Protein, CF Summary
Accession # Q9ULB4
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 250 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||
When Recombinant Human Cadherin 9 Fc Chimera is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human Cadherin-6/KCAD Fc Chimera (Catalog # 2715-CA) binds with an ED50of 0.6-3.6 µg/mL.
2 μg/lane of Recombinant Human Cadherin-9 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 92-106 kDa and 180-210 kDa, respectively.
Cadherin-9 (CDH9) is a member of the larger Cadherin superfamily of cell surface glycoproteins originally identified as proteins mediating cell-cell adhesion (1). In humans, there are more than 100 cadherin members divided into distinct families and numerous sub-families (1-3). Cadherins share a general structural architecture with an extracellular domain (ECD) containing 2 or more extracellular Ca2+ binding cadherin repeat (EC) domains, a single-pass transmembrane section, and a short cytoplasmic tail (1-3). Cadherins function by forming homophilic binding interactions through these EC domains to generate both trans and cis dimers (1-3). Human CDH9 is categorized as a classical cadherin, containing 5 EC domains, and the ECD shares 94% amino acid sequence identity with the ECD of both mouse and rat CDH9, respectively. Cadherins are found in diverse cell types and have been implicated as essential for the morphogenesis and homeostasis of multiple tissues and organs (1-3). Human CDH9 functions primarily in the central nervous system and is expressed in DG and CA3 neurons. Loss of CDH9 expression leads to defects in synapse formation and differentiation of specific neural circuits. (4). Additionally, disruption to CDH9 and CDH10 genes has been linked to autism spectrum disorders (5, 6). CDH9 expression has been identified in human kidney, and it has been used as a cell surface marker for fibroblasts (7). Recently, CDH9 has been reported as a potential suppressor of cancer metastasis (8).
- Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
- Hirano S and Takeichi M. (2012) Physiol Rev. Apr; 92(2):597.
- Nollet et al. (2000) J Mol Biol. Jun. 9; 299(3):551.
- Williams ME et al. (2011) Neuron. Aug. 25; 71(4):640.
- Wang K. et al. (2009) Nature 459, 528.
- Inoue YU and, Inoue T. (2016) Sci Rep. 6:31227.
- Thedieck, C. et al. (2007) PLoS One. 2(8):e657.
- Yan G. et al. (2017) Scientific Reports volume 7, 10023.
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