Recombinant Human Cadherin-9 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9984-CA-050
Recombinant Human Cadherin-9 Fc Chimera Protein, CF Binding Activity
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Recombinant Human Cadherin-9 Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Cadherin-9 Fc Chimera is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human Cadherin‑6/KCAD Fc Chimera (Catalog # 2715-CA) binds with an ED50 of 0.6-3.6 μg/mL. 
Source
Human embryonic kidney cell, HEK293-derived human Cadherin-9 protein
Human Cadherin-9
(Gly54-Ala615)
Accession # Q9ULB4
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Gly54
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
89.1 kDa
SDS-PAGE
92-106 kDa, under reducing conditions

Product Datasheets

9984-CA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Recombinant Human Cadherin-9 Fc Chimera Protein, CF Binding Activity View Larger

WhenRecombinant Human Cadherin 9 Fc Chimera is immobilized at 1 μg/mL, 100 μL/well,Recombinant Human Cadherin‑6/KCAD Fc Chimera (Catalog # 2715-CA) binds with an ED50 of 0.6-3.6μg/mL.

SDS-PAGE Recombinant Human Cadherin-9 Fc Chimera Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Human Cadherin-9 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 92‑106 kDa and 180‑210 kDa, respectively.

Reconstitution Calculator

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Background: Cadherin-9

Cadherin-9 (CDH9) is a member of the larger Cadherin superfamily of cell surface glycoproteins originally identified as proteins mediating cell-cell adhesion (1). In humans, there are more than 100 cadherin members divided into distinct families and numerous sub-families (1-3). Cadherins share a general structural architecture with an extracellular domain (ECD) containing 2 or more extracellular Ca2+ binding cadherin repeat (EC) domains, a single-pass transmembrane section, and a short cytoplasmic tail (1-3). Cadherins function by forming homophilic binding interactions through these EC domains to generate both trans and cis dimers (1-3). Human CDH9 is categorized as a classical cadherin, containing 5 EC domains, and the ECD shares 94% amino acid sequence identity with the ECD of both mouse and rat CDH9, respectively. Cadherins are found in diverse cell types and have been implicated as essential for the morphogenesis and homeostasis of multiple tissues and organs (1-3). Human CDH9 functions primarily in the central nervous system and is expressed in DG and CA3 neurons. Loss of CDH9 expression leads to defects in synapse formation and differentiation of specific neural circuits. (4). Additionally, disruption to CDH9 and CDH10 genes has been linked to autism spectrum disorders (5, 6). CDH9 expression has been identified in human kidney, and it has been used as a cell surface marker for fibroblasts (7). Recently, CDH9 has been reported as a potential suppressor of cancer metastasis (8).

References
  1. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
  2. Hirano S and Takeichi M. (2012) Physiol Rev. Apr; 92(2):597.
  3. Nollet et al. (2000) J Mol Biol. Jun. 9; 299(3):551.
  4. Williams ME et al. (2011) Neuron. Aug. 25; 71(4):640.
  5. Wang K. et al. (2009) Nature 459, 528.
  6. Inoue YU and, Inoue T. (2016) Sci Rep. 6:31227.
  7. Thedieck, C. et al. (2007) PLoS One. 2(8):e657.
  8. Yan G. et al. (2017) Scientific Reports volume 7, 10023.
Entrez Gene IDs
1007 (Human); 12565 (Mouse); 29163 (Rat)
Alternate Names
cadherin 9, type 2 (T1-cadherin); Cadherin9; Cadherin-9; CDH9; T1-Cadherin

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