Recombinant Human Casein Kinase 2 alpha Protein, CF

R&D Systems | Catalog # 7957-CK

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human Casein Kinase 2 alpha Protein (7957-CK)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P68400-1
View all Casein Kinase 2 alpha Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Casein Kinase 2 alpha protein
Asp253-Gln391, with an N-terminal Met and C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

16 kDa

SDS-PAGE

14-16 kDa, reducing conditions

Formulation, Preparation, and Storage

7957-CK
Formulation Supplied as a 0.2 μm filtered solution in MES, NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Casein Kinase 2 alpha

Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein (1). It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm (2). The kinase exists as a tetramer and is composed of an alpha, an alpha ', and two beta subunits (3). The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The Recombinant Human Casein Kinase 2 alpha contains amino acid 253 to 391 of the alpha subunit, which includes an O-GlcNAcylation site at Ser347 (4). O-GlcNAcylation of Casein Kinase 2 alpha not only regulates its kinase substrate specificity, but also regulates the stability of the protein. The Recombinant Human Casein Kinase 2 alpha is an ideal model protein for studying O-GlcNAcylation. It can be O-GlcNAcylated by OGT, the enzyme that introduces O-GlcNAc to target proteins. The introduced O-GlcNAc residue can be detected by Recombinant Human B3GALNT2 (Cat# 1848-GT), a GalNAc transferase that is highly active on O-GlcNAc (5).

References

  1. Ahmad, K.A. et al. (2008) Adv. Enzyme Regul. 48:179.
  2. Rabalski, A.J. et al. (2016) Clinical Cancer Research 22:2840.
  3. Litchfield, D.W. (2003) Biochem. J. 369:1.
  4. Tarrant, M.K. et al. (2012) Nat. Chem. Biol. 8:262.
  5. Wu, Z.L. et al. (2018) Cell Chem. Biol. DOI:https://doi.org/10.1016/j.chembiol.2018.07.007.

Alternate Names

CK2A1, CSNK2A1

Entrez Gene IDs

1457 (Human); 12995 (Mouse); 116549 (Rat)

Gene Symbol

CSNK2A1

UniProt

P68400-1

Additional Casein Kinase 2 alpha Products

Product Documents for Recombinant Human Casein Kinase 2 alpha Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Casein Kinase 2 alpha Protein, CF

For research use only

Related Research Areas

Intracellular Kinases
Notch Pathway

Citations for Recombinant Human Casein Kinase 2 alpha Protein, CF

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail