Recombinant Human CCL7/MCP-3 Protein

R&D Systems | Catalog # 282-P3

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human CCL7/MCP-3 Protein (282-P3)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

Q7Z7Q8

Applications

Bioactivity
View all CCL7/MCP-3/MARC Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human CCL7/MCP-3/MARC protein
Gln34-Leu109

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln34

Predicted Molecular Mass

9 kDa

Activity

Measured by its ability to chemoattract 2-day cultured human monocytes. Matsushima, K. et al. (1989) J. Exp. Med. 169:1485.
The ED50 for this effect is 0.02-0.08 µg/mL.

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR2A.
The ED50 for this effect is 0.1-0.5 µg/mL.

Reviewed Applications

Read 2 reviews rated 5 using 282-P3 in the following applications:

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 282-P3
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 282-P3/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL7/MCP-3/MARC

MCP-2 and CCL7 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and CCL7 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. CCL7 also shares 58% amino acid identity with MCP-2.

CCL7 cDNA encodes a 99 amino acid residue precursor protein from which the N-terminal 23 amino acid residues are cleaved to generate the 76 amino acid residue mature CCL7. Mature CCL7 contains a potential N-linked and several possible O-linked glycosylation sites.

Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Alternate Names

MARC, MCP-3

Entrez Gene IDs

6354 (Human); 20306 (Mouse)

Gene Symbol

CCL7

UniProt

Q7Z7Q8

Additional CCL7/MCP-3/MARC Products

Product Documents for Recombinant Human CCL7/MCP-3 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 282-P3 (with carrier)
Download SDS 282-P3/CF (carrier free)

Product Specific Notices for Recombinant Human CCL7/MCP-3 Protein

For research use only

Citations for Recombinant Human CCL7/MCP-3 Protein

Customer Reviews for Recombinant Human CCL7/MCP-3 Protein (2)

5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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3 Stars
0%
2 Stars
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1 Stars
0%

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    Application: Immunoassay Standard
    Verified Customer | Posted 08/20/2021
    Recombinant Human CCL7/MCP-3 Protein 282-P3
  • Recombinant Human CCL7/MCP-3 Protein
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 11/17/2017
    Recombinant Human CCL7/MCP-3 Protein 282-P3

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