Recombinant Human CD177 Fc Chimera Protein, CF Summary
Accession # AAH29167.1
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human CD177 Fc Chimera Protein (Catalog # 11238-CD) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Podoplanin Fc Chimera Protein (3670-PL) binds with an ED50 of 0.600-7.20 μg/mL.
2 μg/lane of Recombinant Human CD177 Fc Chimera Protein (Catalog # 11238-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 83-93 kDa and 160-180 kDa, respectively.
CD177, also known as NB1, human neutrophil antigen-2a (HNA-2a), and polycythemia rubra vera-1 (PRV-1), is an approximately 50-60 kDa glycoprotein member of the uPAR/CD59/Ly6 superfamily (1). Mature human CD177 is a 387 amino acid (aa) protein that contains two uPAR/Ly6 domains (2, 3). It lacks the first two uPAR domains that are present in mouse and rat CD177. Mature human CD177 shares 55% aa sequence identity with common regions of mouse and rat CD177. Alternative splicing of human CD177 generates additional isoforms that are truncated either just before or just after the first uPAR domain. CD177 is expressed on the surface of neutrophils through a glycosylphosphatidylinositol (GPI) anchor (2, 4, 5). It is nearly absent from neutrophils from paroxysmal nocturnal hemoglobinurea patients who are deficient in the ability to synthesize GPI linkages (5, 7). It is up-regulated on granulocytes from polycythemia vera and thalassemia patients (3, 6). CD177 binds to PECAM-1 on vascular endothelial cells, an interaction which mediates neutrophil adhesion to the vascular wall and neutrophil transmigration (8). It associates in cis with the Integrin MAC-1 (CD11b/CD18) (9). CD177 also associates in cis with Proteinase 3 (PR3) and is required for cell surface PR3 expression (9-11). PR3 is normally found in intracellular vesicles, but once at the cell surface it can serve as an autoimmune target for anti-neutrophil cytoplasmic antibodies (ANCA) (12). The ANCA targeting of CD177-PR3 complexes triggers neutrophil activation and vasculitis (9, 12).
- Stroncek, D.F. (2007) Curr. Opin. Hematol. 14:688.
- Kissel, K. et al. (2001) Eur. J. Immunol. 31:1301.
- Temerinac, S. et al. (2000) Blood 95:2569.
- Skubitz, K.M. et al. (1991) J. Leukoc. Biol. 49:163.
- Klippel, S. et al. (2002) Blood 100:2441.
- Zoi, K. et al. (2008) Brit. J. Haematol. 141:100.
- Boccuni, P. et al. (2000) Crit. Rev. Oncol. Hematol. 33:25.
- Sachs, U.J.H. et al. (2007) J. Biol. Chem. 282:23603.
- Jerke, U. et al. (2011) J. Biol. Chem. 286:7070.
- von Vietinghoff, S. et al. (2007) Blood 109:4487.
- Kuckleburg, C.J. et al. (2012) J. Immunol. 188:2419.
- van Timmeren, M.M. and P. Heeringa (2012) Curr. Opin. Rheumatol. 24:8.
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