Recombinant Human CD277/BTN3A1 Fc Chimera Biotin Protein, CF

R&D Systems | Catalog # BT8539

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Discontinued Product
BT8539 has been discontinued. View all CD277/BTN3A1 products.

Key Product Details

Source

HEK293

Accession Number

O00481

Structure / Form

Disulfide-linked homodimer. Biotinylated via sugars.

Conjugate

Biotin

Applications

Bioactivity
View all CD277/BTN3A1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD277/BTN3A1 protein
Human CD277/BTN3A1
(Phe31-Gly254)
Accession # O00481		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe31

Predicted Molecular Mass

51 kDa (unlabeled)

SDS-PAGE

54-63 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes.
The ED50 for this effect is 1-5 μg/mL.

Formulation, Preparation, and Storage

BT8539
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD277/BTN3A1

Butyrophilin 3A1 (also called BTN3A1) is a 57 kDa type I transmembrane glycoprotein member of the Ig superfamily. It is expressed on a wide variety of immune cells. Similar to BTN3A2 and BTN3A3, BTN3A1 (484 amino acids) is composed of an extracellular N-terminal IgV and a membrane-proximal IgC domain followed by a transmembrane domain and a cytoplasmic tail. These Ig domains are also found in B7 family co-stimulatory molecules, suggesting structural and functional similarities between the two protein families (1). The intracellular portion of BTN3A1 contains a B30.2 domain (2). Although the B30.2 domain of BTN1A1 binds to xanthine oxidoreductase (XOR) and is conserved among BTN1A1 orthologs, this interaction with XOR is not shared by BTN3A1 (3). The B30.2 domain of butyrophilins also functions as a sensor for detecting changes in intracellular phopho-antigen (pAg) concentrations produced during tumorigenesis and microbial infections (4, 5). The specific binding of pAg by the B30.2 domain of BTN3A1 induces a conformational change in its ECD, leading to the activation of V gamma 9V delta 2 T cells (6). Thus, BTN3A1 acts as a critical protein for the activation of V gamma 9V delta 2 T cells following detection of distressed cells (7). The anti-tumor responses of V gamma 9V delta 2 T cells may be enhanced with agonistic anti-BTNA3 antibodies (8). No BTN3A1 homolog has yet been identified in rodents. However, Human BTN3A1 shares 92.4% and 91.6% sequence identity with baboon and rhesus monkey BTN3A1 respectively (9).

References

  1. Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
  2. Rhodes, D.A. et al. (2001) Genomics 71:351.
  3. Jeong, J. et al. (2009) J. Biol. Chem. 284:22444.
  4. Harly, C. et al. (2012) Blood 120:2269.
  5. Constant, P. et al. (1994) Science 264:267.
  6. Sandstrom, A. et al. (2014) Immunity 40:490.
  7. Harly, C. et al. (2015) Front. Immunol. 5:657.
  8. Bonneville, M. et al. (2006) Curr. Opin. Immunol. 18:539.
  9. Wang, H. et al. (2013) J.Immunol. 191:1029.

Alternate Names

BT3.1, BTF5, BTN3A1, CD277

Entrez Gene IDs

11119 (Human)

Gene Symbol

BTN3A1

UniProt

O00481 (Human)

Additional CD277/BTN3A1 Products

Product Documents for Recombinant Human CD277/BTN3A1 Fc Chimera Biotin Protein, CF

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Product Specific Notices for Recombinant Human CD277/BTN3A1 Fc Chimera Biotin Protein, CF

For research use only

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