Recombinant Human CD300c Fc Chimera Protein, CF

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Recombinant Human CD300c Fc Chimera Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti‑CD3 and Recombinant Human CD300c Fc Chimera.
The ED50 for this effect is 4-16 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.
Mouse myeloma cell line, NS0-derived human CD300c protein
Human CD300c
Accession # Q08708
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
43.5 kDa (monomer)
60-70 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CD300c

Leukocyte mono-Ig-like receptor 2 (LMIR2; also CMRF-35, CMRF35-A antigen, and CD300c antigen) is a 23 kDa (predicted) type I transmembrane glycoprotein that belongs to the immunoregulatory signaling (IRS) family of molecules within the immunoglobulin (Ig) superfamily (1-4). Human LMIR2 is synthesized as a 224 amino acid (aa) precursor that has a 20 aa signal sequence, a 163 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 20 aa cytoplasmic tail (SwissProt # Q08708). The ECD contains an Ig-like V-type domain (aa’s 22-128) and two N-linked glycosylation sites (aa’s 90 and 99). Downstream of the Ig V-domain, the membrane proximal region of LMIR2 (aa 128-183) contains a high proportion of proline (18%), serine (20%) and threonine (13%) residues (1). The transmembrane segment contains a charged glutamic acid that contributes to cell activation (1-3). Human LMIR2 shares 52% aa sequence identity with the mouse LMIR2, which is also known as CLM4. Human LMIR2 is 90% identical to human LMIR1 within the Ig-like domain. LMIR2 is present on the surface of natural killer cells, granulocytes, most myeloid cells, dendritic cells, and a subpopulation of T and B lymphocytes (1, 3). Mouse LMIR2 has the characteristics of an activatory molecule capable of inducing cellular activation and effector function in most cells and macrophages (3). The ligand for LMIR2 is presently unknown.

  1. Jackson, D.G. et al. (1992) Eur. J. Immunol. 22:1157.
  2. Clark, G.J. et al. (2001) Tissue Antigens 57:415.
  3. Clark, G.J. et al. (2002) J. Biol. Regul. Homeost. Agents 16:233.
  4. Daish, A. et al. (1993) Immunology 79:55.
Long Name
CD300 Antigen-Like Family Member C
Entrez Gene IDs
10871 (Human); 387565 (Mouse)
Alternate Names
CD300c antigenCMRF35 antigen; CD300c molecule; CD300c; CLM6; CLM-6; CMRF35 leukocyte immunoglobulin-like receptor; CMRF35; CMRF-35; CMRF35A leukocyte immunoglobulin-like receptor; CMRF-35A; CMRF35ACMRF35A1; CMRF35CMRF35-A1; CMRF35-like molecule 6; IGSF16 ; IgSF16; IGSF16CD300 antigen-like family member C; Immunoglobulin superfamily member 16; LIR


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