Recombinant Human CD320/TCblR/8D6A Fc Chimera Protein, CF

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Recombinant Human CD320/TCblR/8D6A Fc Chimera Protein Bioactivity
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Recombinant Human CD320/TCblR/8D6A Fc Chimera Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human CD320/TCblR/8D6A Fc Chimera is immobilized at 25 ng/mL (100 μL/well), the concentration of Recombinant Human Transcobalamin II (Catalog # 7895-TC) that produces 50% of the optimal binding response is 0.5-3 ng/mL.
Mouse myeloma cell line, NS0-derived human CD320/TCblR/8D6A protein
Human CD320
Accession # Q9NPF0
N-terminus C-terminus
Accession #
N-terminal Sequence
Leu31 and Ser36
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
47.2 kDa (monomer)
80-90 kDa, reducing conditions

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Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human CD320/TCblR/8D6A Fc Chimera Protein Bioactivity View Larger

When Recombinant Human CD320/TCblR/8D6A Fc Chimera (Catalog # 1557-CD) is coated at 25 ng/mL, Recombinant Human Transcobalamin II (Catalog # 7895-TC) binds with an ED50 of 0.5-3 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: CD320/TCblR/8D6A

CD320, also known as the Transcobalamin II receptor (TCb1R) and 8D6A, is an approximately 60 kDa transmembrane member of the LDL receptor family (1). Mature human CD320 consists of a 199 amino acid (aa) extracellular domain with two cysteine-rich LDLR-A repeats, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2). Within the extracellular domain, human CD320 shares 52% aa sequence identity with mouse and rat CD320. CD320 is a heavily glycosylated protein that is expressed as a noncovalent dimer in the kidney, placenta, liver, intestine, and secondary lymphoid organs (3 - 5). It functions as an endocytic receptor for circulating complexes of Cobalamin (Vitamin B12) and Transcobalamin II (3). In the immune system, CD320 is expressed by germinal center B cells and follicular dendritic cells (FDC) and provides a co‑stimulatory signal to B cells during the germinal center reaction (2, 6 - 8). CD320 also cooperates with CD44 in promoting the development of B cell lymphomas (9).

  1. May, P. et al. (2007) Ann. Med. 39:219.
  2. Li, L. et al. (2000) J. Exp. Med. 191:1077.
  3. Quadros, E.V. et al. (2009) Blood 113:186.
  4. Bose, S. et al. (1995) J. Biol. Chem. 270:8152.
  5. Park, H.J. et al. (2009) Immune Netw. 9:138.
  6. Cho, W. et al. (2008) BMC Rep. 41:863.
  7. Zhang, X. et al. (2001) J. Immunol. 167:49.
  8. Park, C.-S. and Y.S. Choi (2005) Immunology 114:2.
  9. Li, L. et al. (2004) Blood 104:815.
Long Name
Transcobalamin II Receptor
Entrez Gene IDs
51293 (Human); 54219 (Mouse); 362851 (Rat)
Alternate Names
8D6 antigen; 8D6A; 8D6AFDC-SM-8D6; CD320 antigenFDC-signaling molecule 8D6,8D6TCBLR; CD320 molecule; CD320; TCblR; Transcobalamin receptor


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