Recombinant Human CD320/TCblR/8D6A Fc Chimera Protein, CF Summary
Accession # Q9NPF0
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human CD320/TCblR/8D6A Fc Chimera (Catalog # 1557-CD) is coated at 25 ng/mL, Recombinant Human Transcobalamin II (Catalog # 7895-TC) binds with an ED50 of 0.5-3 ng/mL.
CD320, also known as the Transcobalamin II receptor (TCb1R) and 8D6A, is an approximately 60 kDa transmembrane member of the LDL receptor family (1). Mature human CD320 consists of a 199 amino acid (aa) extracellular domain with two cysteine-rich LDLR-A repeats, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2). Within the extracellular domain, human CD320 shares 52% aa sequence identity with mouse and rat CD320. CD320 is a heavily glycosylated protein that is expressed as a noncovalent dimer in the kidney, placenta, liver, intestine, and secondary lymphoid organs (3 - 5). It functions as an endocytic receptor for circulating complexes of Cobalamin (Vitamin B12) and Transcobalamin II (3). In the immune system, CD320 is expressed by germinal center B cells and follicular dendritic cells (FDC) and provides a co‑stimulatory signal to B cells during the germinal center reaction (2, 6 - 8). CD320 also cooperates with CD44 in promoting the development of B cell lymphomas (9).
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- Li, L. et al. (2000) J. Exp. Med. 191:1077.
- Quadros, E.V. et al. (2009) Blood 113:186.
- Bose, S. et al. (1995) J. Biol. Chem. 270:8152.
- Park, H.J. et al. (2009) Immune Netw. 9:138.
- Cho, W. et al. (2008) BMC Rep. 41:863.
- Zhang, X. et al. (2001) J. Immunol. 167:49.
- Park, C.-S. and Y.S. Choi (2005) Immunology 114:2.
- Li, L. et al. (2004) Blood 104:815.
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