Recombinant Human CD35 Protein, CF
Recombinant Human CD35 Protein, CF Summary
Gln42-Asp1971, with a C-terminal 10-His tag and a substitution of His1208Arg
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 400 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
CD35, also known as complement receptor 1 (CR1), is an N-glycosylated member of the RCA (regulators of complement activation) family of proteins. The complement cascade plays an important role in the innate immune system through the recognition and clearance of immune complexes and foreign particles. There are four major allotypes of human CD35 which range from approximately 220 - 300 kDa (1, 2). Mature human CD35 contains a 1930 amino acid (aa) extracellular domain (ECD) with 30 tandem SCR/SUSHI repeats, a 25 aa transmembrane segment, and a 43 aa cytoplasmic tail (3). A mouse ortholog of human CD35 has not been described, although alternate splicing of mouse CD21/CR2 generates a protein with homology to some SCR repeats of human CD35 (4). A soluble form of the CD35 ECD circulates in the serum (5). Cell surface CD35 is widely expressed on hematopoietic cells (1). It cooperates with both CD21 and CR3/MAC-1 in the binding and internalization of particles and immune complexes that are opsonized with complement components C3b, C3i, and C4b (1, 6, 7). It also binds and internalizes particles opsonized with complement factor C1q or mannose binding lectin (MBL) (8, 9). CD35 protects the cell from complement-mediated lysis by serving as a cofactor for Factor I and inhibiting the C3 and C5 convertases (10). Erythrocyte-expressed CD35 mediates the hepatic clearance of complement-opsonized immune complexes (11). On lymphocytes, CD35 inhibits the activation-induced proliferation of T cells and B cells (12, 13). Mice deficient in both CD21 and CD35 exhibit normal B cell development but severely compromised germinal center development, antibody production, and establishment of protective microbial immunity (14). In mice, CD21/CD35 must additionally be present on follicular dendritic cells to mount effective humoral responses and establishment of B cell memory (15).
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- Klickstein, L.B. et al. (1997) Immunity 7:345.
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Citations for Recombinant Human CD35 Protein, CF
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Citations: Showing 1 - 3
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Tissue plasminogen activator mediates deleterious complement cascade activation in stroke
Authors: XJ Zhao, TM Larkin, MA Lauver, S Ahmad, AF Ducruet
PLoS ONE, 2017;12(7):e0180822.
Sample Types: Proteins
Three pentraxins C-reactive protein, serum amyloid p component and pentraxin 3 mediate complement activation using Collectin CL-P1
Authors: Nobutaka Wakamiya
Biochim. Biophys. Acta, 2016;0(0):.
Sample Types: Whole Cells
Complement factor H-related hybrid protein deregulates complement in dense deposit disease.
Authors: Chen, Qian, Wiesener, Michael, Eberhardt, Hannes U, Hartmann, Andrea, Uzonyi, Barbara, Kirschfink, Michael, Amann, Kerstin, Buettner, Maike, Goodship, Tim, Hugo, Christia, Skerka, Christin, Zipfel, Peter F
J Clin Invest, 2014;124(1):145-55.
Sample Types: Serum
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