Recombinant Human CD35 Protein, CF

  (2 citations)     
Datasheet / CoA / SDS
Product Details
Citations (2)
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  • Purity
    >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When rhCD35 is coated at 5 μg/mL, complement component C3b binds with an apparent KD <10 nM.
  • Source
    Mouse myeloma cell line, NS0-derived
    Gln42-Asp1971, with a C-terminal 10-His tag and a substitution of His1208Arg
  • Accession #
  • N-terminal Sequence
    Analysis
    No results obtained: Gln42 predicted
  • Predicted Molecular Mass
    213 kDa
  • SDS-PAGE
    200-220 kDa, reducing conditions
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5748-CD
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD35
CD35, also known as complement receptor 1 (CR1), is an N-glycosylated member of the RCA (regulators of complement activation) family of proteins. The complement cascade plays an important role in the innate immune system through the recognition and clearance of immune complexes and foreign particles. There are four major allotypes of human CD35 which range from approximately 220 - 300 kDa (1, 2). Mature human CD35 contains a 1930 amino acid (aa) extracellular domain (ECD) with 30 tandem SCR/SUSHI repeats, a 25 aa transmembrane segment, and a 43 aa cytoplasmic tail (3). A mouse ortholog of human CD35 has not been described, although alternate splicing of mouse CD21/CR2 generates a protein with homology to some SCR repeats of human CD35 (4). A soluble form of the CD35 ECD circulates in the serum (5). Cell surface CD35 is widely expressed on hematopoietic cells (1). It cooperates with both CD21 and CR3/MAC-1 in the binding and internalization of particles and immune complexes that are opsonized with complement components C3b, C3i, and C4b (1, 6, 7). It also binds and internalizes particles opsonized with complement factor C1q or mannose binding lectin (MBL) (8, 9). CD35 protects the cell from complement-mediated lysis by serving as a cofactor for Factor I and inhibiting the C3 and C5 convertases (10). Erythrocyte-expressed CD35 mediates the hepatic clearance of complement-opsonized immune complexes (11). On lymphocytes, CD35 inhibits the activation-induced proliferation of T cells and B cells (12, 13). Mice deficient in both CD21 and CD35 exhibit normal B cell development but severely compromised germinal center development, antibody production, and establishment of protective microbial immunity (14). In mice, CD21/CD35 must additionally be present on follicular dendritic cells to mount effective humoral responses and establishment of B cell memory (15).
  • References:
    1. Roozendaal, R. and M.C. Carroll (2007) Immunol. Rev. 219:157.
    2. Wong, W.W. et al. (1989) J. Exp. Med. 169:847.
    3. Klickstein, L.B. et al. (1987) J. Exp. Med. 165:1095.
    4. Kurtz, C.B. et al. (1990) J. Immunol. 144:3581.
    5. Yoon, S.H. and D.T. Fearon (1985) J. Immunol. 134:3332.
    6. Tuveson, D.A. et al. (1991) J. Exp. Med. 173:1083.
    7. Sutterwala, F.S. et al. (1996) J. Leukoc. Biol. 59:883.
    8. Klickstein, L.B. et al. (1997) Immunity 7:345.
    9. Ghiran, I. et al. (2000) J. Exp. Med. 192:1797.
    10. Iida, K. and V. Nussenzweig (1981) J. Exp. Med. 153:1138.
    11. Cornacoff, J.B. et al. (1983) J. Clin. Invest. 71:236.
    12. Wagner, C. et al. (2006) Mol. Immunol. 43:643.
    13. Jozsi, M. et al. (2002) J. Immunol. 168:2782.
    14. Haas, K.M. et al. (2002) Immunity 17:713.
    15. Rossbacher, J. et al. (2006) Eur. J. Immunol. 36:2384.
  • Entrez Gene IDs:
    1378 (Human)
  • Alternate Names:
    C3BR; CD35; complement component (3b/4b) receptor 1 (Knops blood group); CR1; KN
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Tissue plasminogen activator mediates deleterious complement cascade activation in stroke
    Authors: XJ Zhao, TM Larkin, MA Lauver, S Ahmad, AF Ducruet
    PLoS ONE, 2017;12(7):e0180822.
    Species: N/A
    Sample Type: Proteins
    Application: Bioassay
  2. Complement factor H-related hybrid protein deregulates complement in dense deposit disease.
    Authors: Chen, Qian, Wiesener, Michael, Eberhardt, Hannes U, Hartmann, Andrea, Uzonyi, Barbara, Kirschfink, Michael, Amann, Kerstin, Buettner, Maike, Goodship, Tim, Hugo, Christia, Skerka, Christin, Zipfel, Peter F
    J Clin Invest, 2014;124(1):145-55.
    Species: Human
    Sample Type: Serum
    Application: Bioassay

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Primary Antibodies
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His Tag Antibody

WB , Simple Western , Flow , AP , CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB , Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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ICFlow IC050C  
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ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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