Recombinant Human CD46 Fc Chimera Protein, CF Summary
|Human CD46 |
Accession # P15529-11
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human CD46 Fc Chimera (Catalog # 10257-CD) is immobilized at 5 μg/mL (100 μL/well), it binds to Human C4b with an ED50 of 2‑12 μg/mL.
2 μg/lane of Recombinant Human CD46 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 88-98 kDa and 160-190 kDa, respectively.
CD46, also known as MCP (Membrane cofactor protein), is a type I membrane protein that functions as a cofactor for Complement Factor Imediated inactivation of complement components C3b and C4b (1, 2). Mature human CD46 consists of a 309 amino acid (aa) extracellular domain (ECD) with four short consensus repeats (SCR) and a Ser/Thr/Pro-enriched region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain (1). Within the ECD, human CD46 shares 50% aa sequence identity with mouse and rat CD46. CD46 is commonly expressed in four distinct isoforms, and all isoforms have conserved complement control protein domains which harbor the C3b and C4b binding sites (3). CD46 is expressed on all nucleated cells (4). CD46 participates in the maintenance of immune homeostasis and the induction of protective T cell immunity, and it could extend towards key processes in development and cell metabolism (5).
- Liszewski, M.K. et al. (1991) Annu. Rev. Immunol. 9:431.
- Barilla-LaBarca, M.L. et al. (2002) J. Immunol. 168:6298.
- Yamamoto, H. et al. (2013) Intl. J. Biochem. Cell Biol. 45:2808.
- Andrews, P.W. et al. (1985) Ann. Hum. Genet. 49:31.
- Kolev, M. et al. (2013) Semin. Immunol. 25:12.
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