Recombinant Human CD47 Fc Chimera Protein, CF

R&D Systems | Catalog # 4670-CD

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human CD47 Fc Chimera Protein (4670-CD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q08722

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all CD47 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CD47 protein
Human CD47
(Gln19-Pro139)
Accession # Q08722		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln19 predicted

Predicted Molecular Mass

40.3 kDa (monomer)

SDS-PAGE

60-65 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human (rh) SIRP alpha /CD172a Fc Chimera (Catalog # 4546-SA) is coated at 2 μg/mL, Recombinant Human CD47 Fc Chimera binds with an apparent Kd <1 nM. 

Optimal dilutions should be determined by each laboratory for each application.



Also measured by its ability to antagonize red blood cell adhesion to immobilized rhSIRP alpha Fc Chimera. The ED50 for this effect is 0.5-2.0 μg/mL.

Reviewed Applications

Read 1 review rated 4 using 4670-CD in the following applications:

Formulation, Preparation, and Storage

4670-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD47

CD47, also known as Integrin-Associated Protein (IAP) and OA3, is a 40-60 kDa variably glycosylated atypical member of the immunoglobulin superfamily (1, 2). Human CD47 is an integral membrane protein that consists of a 123 amino acid (aa) extracellular domain (ECD) with a single Ig-like domain, five membrane-spanning regions with short intervening loops, and a 34 aa C-terminal cytoplasmic tail (3). Alternate splicing of human CD47 generates additional isoforms with deletions in the cytoplasmic tail (3). Within the N-terminal ECD, human CD47 shares 63% aa sequence identity with mouse and rat CD47. A portion of the N‑terminal ECD can by shed from smooth muscle cells by MMP-2-mediated proteolysis (4). The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells (5, 6). These interactions prevent macrophage-mediated clearance of healthy CD47-expressing cells and promote immune cell transmigration across the vascular endothelium (5-8). The CD47-SIRP alpha interaction is species specific, and this lack of cross-species interaction has been implicated in xenotransplantation rejection (16). CD47 associates in cis with Fas on T cells and enhances Fas‑mediated apoptosis; its ligation promotes T cell anergy and dampens Th1 immune responses (9-11). CD47 also associates in cis with Integrins  alpha 4 beta 1, alpha V beta 3, alpha 2b beta 3, and  alpha 2 beta 1 which can positively or negatively modulate Integrin‑mediated function (2, 12). In the vasculature, CD47 binding by Thrombospondin-1 inhibits the angiogenic and vasorelaxant effects of nitric oxide (2, 13, 14). On dendritic cells and myeloma cells, CD47 ligation by TSP-1 induces giant cell formation and osteoclast differentiation (15).

References

  1. Sarfati, M. et al. (2009) Curr. Drug Targ. 9:842.
  2. Isenberg, J.S. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:615.
  3. Campbell, I.G. et al. (1992) Cancer Res. 52:5416.
  4. Maile, L.A. et al. (2008) Mol. Endocrinol. 22:1226.
  5. Oldenborg, P.-A. et al. (2000) Science 288:2051.
  6. Liu, Y. et al. (2002) J. Biol. Chem. 277:10028.
  7. Stefanidakis, M. et al. (2008) Blood 112:1280.
  8. de Vries, H.E. et al. (2002) J. Immunol. 168:5832.
  9. Manna, P.P. et al. (2005) J. Biol. Chem. 280:29637.
  10. Avice, M.-N. et al. (2001) J. Immunol. 167:2459.
  11. Bouguermouh, S. et al. (2008) J. Immunol. 180:8073.
  12. Barazi, H.O. et al. (2002) J. Biol. Chem. 277:42859.
  13. Isenberg, J.S. et al. (2009) Nitric Oxide 21:52.
  14. Isenberg, J.S. et al. (2009) Matrix Biol. 28:110.
  15. Kukreja, A. et al. (2009) Blood 114:3413.
  16. Wang H. et al. (2007) Blood 109:836.

Alternate Names

CD47, IAP, MER6, OA3

Entrez Gene IDs

961 (Human); 16423 (Mouse); 397042 (Porcine); 478552 (Canine); 102139846 (Cynomolgus Monkey)

Gene Symbol

CD47

UniProt

Q08722

Additional CD47 Products

Product Documents for Recombinant Human CD47 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human CD47 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human CD47 Fc Chimera Protein, CF

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  • Recombinant Human CD47 Fc Chimera Protein, CF
    Name: haibin Chen
    Application: Raising antibodies against human CD47
    Verified Customer | Posted 01/13/2016

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