Recombinant Human CD5L Protein, CF Summary
Ser20-Gly347, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
CD5L (CD5 antigen-like), also known as Sp alpha and AIM, is a 50 kDa secreted glycoprotein that belongs to the SRCR (scavenger receptor cysteine rich) group B family of proteins. Group B proteins are distinguished by SRCR domains that are encoded by a single exon (1 - 3). The human CD5L cDNA encodes a 347 amino acid (aa) precursor that includes a 19 aa signal sequence and three SRCR domains (4, 5). Among group B proteins, CD5L is most closely related to CD5 and CD6, with which it shares 18% and 31% aa sequence identity, respectively. CD5L is up-regulated in macrophages at inflammatory sites. It sustains inflammatory reactions by both increasing the phagocytic capacity of macrophages and impeding the apoptosis of local macrophages, NK cells, and T cells (6, 7). Agonists of the LXR and RXR nuclear hormone receptors induce CD5L upregulation in macrophages and reduce macrophage apoptosis (8, 9). Oxidized LDL (which acts through LXR/RXR) is taken up by macrophages, promoting their development into foam cells. The increased level of CD5L protects foam cells from apoptosis but permits more rapid cellular accumulation and atherosclerotic plaque formation (9). In activated B cells, however, the combination of CD5L and TGF-beta inhibits proliferation. The binding of CD5L to splenic B cells is increased following TGF-beta exposure, suggesting that TGF-beta increases the expression or availability of an unidentified CD5L receptor (5, 10). CD5L also functions as a pattern recognition molecule by binding both lipoteichoic acid on Gram positive and lipopolysaccharide on Gram negative bacteria (11). In the thymic cortex, CD5L protects cortical CD4+CD8+ thymocytes from apoptosis (12). CD5L circulates in the serum in complex with IgM (13).
- Sarrias, M.R. et al. (2004) Crit. Rev. Immunol. 24:1.
- Resnick, D. et al. (1994) Trends Biochem. Sci. 19:5.
- Mukhopadhyay, S. and S. Gordon (2004) Immunobiology 209:39.
- Gebe, J.A. et al. (1997) J. Biol. Chem. 272:6151.
- Gebe, J.A. et al. (2000) Immunol. 99:78.
- Haruta, I. et al. (2001) J. Biol. Chem. 276:22910.
- Kuwata, K. et al. (2003) Am. J. Pathol. 162:837.
- Valledor, A.F. et al. (2004) Proc. Natl. Acad. Sci. 101:17813.
- Arai, S. et al. (2005) Cell Metab. 1:201.
- Yusa, S. et al. (1999) Eur. J. Immunol. 29:1086.
- Sarrias, M-R. et al. (2005) J. Biol. Chem. 280:35391.
- Miyazaki, T. et al. (1999) J. Exp. Med. 189:413.
- Tissot, J.D. et al. (2002) Electrophoresis 23:1203.
Citations for Recombinant Human CD5L Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
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Recombinant apoptosis inhibitor of macrophage protein reduces delayed graft function in a murine model of kidney transplantation
Authors: JY Lee, S Arumugaraj, D Lian, N Maehara, AR Haig, RS Suri, T Miyazaki, L Gunaratnam
PLoS ONE, 2021;16(4):e0249838.
Sample Types: Whole Cells
Applications: Flow Cytometry
Role of the Scavenger Receptor CD36 in Accelerated Diabetic Atherosclerosis
Authors: M Navas-Madr, E Castelblan, M Camacho, M Consegal, A Ramirez-Mo, MR Sarrias, P Perez, N Alonso, M Galán, D Mauricio
Int J Mol Sci, 2020;21(19):.
Sample Types: Whole Cells
Applications: Cell Culture
Retinal pigment epithelium and microglia express the CD5 antigen-like protein, a novel autoantigen in age-related macular degeneration
Authors: Alessandro Iannaccone
Exp. Eye Res, 2016;0(0):.
Applications: ELISA (Capture)
Human scavenger protein AIM increases foam cell formation and CD36-mediated oxLDL uptake.
Authors: Amezaga N, Sanjurjo L, Julve J, Aran G, Perez-Cabezas B, Bastos-Amador P, Armengol C, Vilella R, Escola-Gil J, Blanco-Vaca F, Borras F, Valledor A, Sarrias M
J Leukoc Biol, 2013;95(3):509-20.
Sample Types: Whole Cells
A proteomic strategy to identify novel serum biomarkers for liver cirrhosis and hepatocellular cancer in individuals with fatty liver disease.
Authors: Gray J, Chattopadhyay D, Beale GS
BMC Cancer, 2009;9(0):271.
Sample Types: N/A
Applications: ELISA (Standard)
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