Recombinant Human CD82 Fc Chimera Protein, CF Summary
Accession # P27701
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
2 μg/lane of Recombinant Human CD82/Kai-1 Fc Chimera was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 53-65 kDa and 105-130 kDa, respectively.
CD82 antigen, also known as Kai-1, C33 and TSPAN27, is a widely expressed palmitoylated molecule of the tetraspanin superfamily (1, 2). The general architecture of tetraspanins contain four transmembrane domains, two extracellular loops, a small inner cytoplasmic loop, and intra-cellular N and C termini (1). One of the extracellular loops is termed the large extracellular loop (LEL) and it is responsible for interactions with binding partners such as integrins. Within the LEL, human CD82/Kai-1 shares 65% and 63%amino acid (aa) sequence identity with mouse and rat CD82/Kai-1, respectively. CD82/Kai-1 is a component of the promiscuous TIMP-1 interacting protein complex on the cell surface of human adenocarcinoma cells and gives insight into tumorigenic metastatic potential (3). CD82/Kai-1 suppresses EMT in prostate cancer cells adhered to fibronectin leading to reduced cell migration and invasiveness (4). CD82/Kai-1 function is important for muscle stem cell function in muscular disorders (5). Overexpression of CD82/Kai-1 suppresses growth, migration and invasion of oral cancer cells and may be considered as a potential therapeutic target in oral cancer (6).
- Mazurov, D. et al. (2006) J. Biol. Chem. 282:3896.
- Berditchevski, F. et al. 2001, J Cell Sci. 114:4143.
- Zang, J. et al. (2017) Oncotarget. 8:6496.
- Lee, J. et al. (2017) Oncotarget. 8:1641.
- Alexander, MS. et al. (2016) Cell Stem Cell. 19:800.
- Chai, J. et al. (2017) Mol Med. Rep. April; 15:1527.
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