Recombinant Human CDO Protein, CF

Catalog # Availability Size / Price Qty
4384-CD-050
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Recombinant Human CDO Protein, CF Summary

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When rhSHH (2 µg/mL) is incubated with serial dilutions of rhCDO in solution, rhCDO binds rhSHH with an apparent KD <30 nM.
Source
Mouse myeloma cell line, NS0-derived human CDO protein
Asp26-Pro943 (Leu669Ile), with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Asp26
Predicted Molecular Mass
100.2 kDa
SDS-PAGE
130-140 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4384-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: CDO

CDO (CAM-related/down-regulated by oncogenes, also CDON; pronounced “kid-oh”) is a 190 kDa member of the Immunoglubulin (Ig) superfamily, Ig/Fibronectin (FN) type III repeat family of cell surface proteins (1). Human CDO is a type I transmembrane (TM) glycoprotein. It is synthesized as a 1287 amino acid (aa) precursor that contains a 25 aa signal sequence, a 938 aa extracellular domain (ECD), a 21 aa TM segment and a 303 aa cytoplasmic region (1, 2). The ECD contains five C2-type Ig-like domains, followed by three FN type III repeats. The first FN repeat (aa 577 - 673) is known to bind numerous cadherins, while the third (or juxtramembrane) FN type III repeat (aa 826 - 923) binds SHH (3, 4). The intracellular region is believed to signal through various bHLH transcription factors (2). One alternate splice form is reported that shows a deletion of aa 1212 - 1234 in the cytoplasmic tail. The ECD of human CDO is 85% aa identical to mouse CDO ECD. CDO is found on muscle precursor and neural progenitor cells of the embryo (5, 6). It likely promotes muscle differentiation, and contributes to axon guidance and neuronal patterning (2, 7, 8, 9). These effects may be mediated through two different receptor complexes. On muscle precursors, CDO apparently acts as both a coordinating and signaling subunit. Here, it integrates N- and M-cadherin, neogenin, netrin-3 and BOC into a cis-oriented receptor complex (2). While this complex has no identified ligand, intercellular cadherin interactions or netrin, may be enough to trigger CDO/cadherin/neogenin signaling. On axons, CDO may participate in a poorly-defined receptor complex minimally composed of CDO, BOC and Gas1 that binds SHH, and interacts with PTCH1 (7, 8, 10).

References
  1. Kang, J.S. et al. (1997) J. Cell Biol. 138:203. 
  2. Krauss, R.S. et al. (2005) J. Cell Sci. 118:2355. 
  3. Yao, S. et al. (2006) Cell 125:343. 
  4. 4.Kang, J-S. et al. (2003) Proc. Natl. Acad. Sci. USA 100:3989. 
  5. Kang, J-S. et al. (2002) EMBO J. 21:114. 
  6. Zhang, W. et al. (2006) Mol. Cell. Biol. 26:3764.
  7. Okada, A. et al. (2006) Nature 444:369.
  8. Allen, B.L. et al. (2007) Genes Dev. 21:1244.
  9. Kang, J-S. et al. (2004) J. Cell. Biol. 167:493.
  10. Tenzen, T. et al. (2006) Dev. Cell 10:647.
Long Name
Cell Adhesion Molecule-related/Down-regulated by Oncogenes
Entrez Gene IDs
50937 (Human); 57810 (Mouse)
Alternate Names
CDO; Cdon homolog (mouse); CDON; CDOORCAM; cell adhesion molecule-related/down-regulated by oncogenes; MGC111524; ORCAM; surface glycoprotein, Ig superfamily member

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