Recombinant Human Chitinase 3-like 1/YKL-40 Protein, CF

R&D Systems | Catalog # 2599-CH

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human Chitinase 3-like 1/YKL-40 Protein (2599-CH)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P36222

Applications

Bioactivity
View all Chitinase 3-like 1/YKL-40 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Chitinase 3-like 1/YKL-40 protein
Tyr22-Thr383, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr22

Predicted Molecular Mass

41.3 kDa (monomer)

SDS-PAGE

38-41 kDa, reducing conditions

Activity

Bioassay data are not available.

Reviewed Applications

Read 2 reviews rated 5 using 2599-CH in the following applications:

Formulation, Preparation, and Storage

2599-CH
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Chitinase 3-like 1/YKL-40

Chitinase 3-like 1 (CHI3L1), also called YKL-40 or human cartilage glycoprotein 39 (HCGP39), is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family (1-4). CHI3L1 was first identified as secreted from cultured articular chondrocytes, synovial cells, and activated monocyte-derived macrophages, but it is also secreted by neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells (1, 2, 5-7). The human CHI3L1 cDNA encodes 383 amino acids (aa), including a 21 aa signal sequence and a 362 aa mature region with two intramolecular disulfides (3). Mature human CHI3L1 shares 73%, 80%, 82%, 83%, 86% and 87% aa sequence identity with mouse, rat, canine, bovine, porcine and equine CHI3L1, respectively. Human CHI3L1 and CHI3L2 share 43% aa sequence identity. Neither shows chitotriosidase activity, but both bind chitin and are thus termed chi-lectins (1-4). CHI3L1 can bind heparins, probably as heparan sulfate (3, 7). It has been found to enhance cell adhesion and promote cell signaling, proliferation and tumor angiogenesis (4, 6-9). Elevated serum CHI3L1 levels occur in some conditions characterized by inflammation and connective tissue remodeling, such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis (1, 9-12). Single nucleotide polymorphisms that can increase serum CHI3L1 are associated with higher risk for asthma in childhood (13). CHI3L1 is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary (9). In asthma and cancer, serum CHI3L1 concentrations can correlate with prognosis (9, 14, 15).

References

  1. Hakala, B.E. et al. (1993) J. Biol. Chem. 268:25803.
  2. Renkema, G.H. et al. (1998) Eur. J. Biochem. 251:504.
  3. Fusetti, F. et al. (2003) J. Biol. Chem. 278:37753.
  4. Houston, D.R. et al. (2003) J. Biol. Chem. 278:30206.
  5. Volck, B. et al. (1998) Proc. Assoc. Am. Physicians 110:351.
  6. Nishikawa, K.C. and A.J.T. Millis (2003) Exp. Cell Res. 287:79.
  7. Shao, R. et al. (2009) Oncogene 28:4456.
  8. Recklies, A.D. et al. (2002) Biochem. J. 365:119.
  9. Eurich, K. et al. (2009) World J. Gastroenterol. 15:5249.
  10. Letuve, S. et al. (2008) J Immunol. 181:5167.
  11. Rathke, C.N. and H. Vestergaard (2009) Cardiovasc. Diabetol. 8:61.
  12. Johansen, J.S. et al. (1997) Scand. J. Gastroenterol. 32:582.
  13. Ober, C. et al. (2008) N. Engl. J. Med. 358:1682.
  14. Johansen, J.S. et al. (1999) Rheumatology 38:618.
  15. Chupp, G.L. et al. (2007) N. Engl. J. Med. 357:2016.

Alternate Names

CHI3L1, Chitinase 3 like 1, HCgp39, YKL-40

Entrez Gene IDs

1116 (Human); 12654 (Mouse); 102138036 (Cynomolgus Monkey)

Gene Symbol

CHI3L1

UniProt

P36222

Additional Chitinase 3-like 1/YKL-40 Products

Product Documents for Recombinant Human Chitinase 3-like 1/YKL-40 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human Chitinase 3-like 1/YKL-40 Protein, CF

For research use only

Citations for Recombinant Human Chitinase 3-like 1/YKL-40 Protein, CF

Customer Reviews for Recombinant Human Chitinase 3-like 1/YKL-40 Protein, CF (2)

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  • Recombinant Human Chitinase 3-like 1 Protein, CF
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 11/06/2018
    The recombinant Chitinase 3-like 1 (YKL-40) worked well as a standard in an ELISA made to measure YKL-40 in human serum samples.
    Recombinant Human Chitinase 3-like 1/YKL-40 Protein, CF 2599-CH
  • Recombinant Human Chitinase 3-like 1 Protein, CF
    Name: Anonymous
    Application: LCMS Biomarker discovery
    Verified Customer | Posted 09/05/2018
    We use this item as a positive control for IP experiments.
    Recombinant Human Chitinase 3-like 1/YKL-40 Protein, CF 2599-CH

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