Recombinant Human Chondrolectin His-tag Protein, CF

Catalog # Availability Size / Price Qty
Recombinant Human Chondrolectin His-tag Protein Binding Activity.
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Product Details

Recombinant Human Chondrolectin His-tag Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human Chondrolectin His-tag Protein is immobilized at 1.00 ug/mL (100 µL/well), Recombinant Human IGFBP-5 binds with an ED50 of 5.00-30.0 ng/mL.
Mouse myeloma cell line, NS0-derived human Chondrolectin protein
Arg22-Asn216, with C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
23 kDa
34-40 kDa, under reducing conditions.

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 1.00 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When Recombinant Human Chondrolectin His-tag Protein (Catalog # 11059-CL) is immobilized at 1.00 ug/mL (100 µL/well), Recombinant Human IGFBP-5 (875-B5) binds with an ED50 of 5.00󈛂.0 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human Chondrolectin His-tag Protein (Catalog # 11059-CL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 34-40 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Chondrolectin

Chondrolectin (CHODL), also known as transmembrane protein MT75, is a type I transmembrane protein belonging to the C-type lectin-like domain superfamily. CHODL is predominantly expressed by the vascular muscle of the testis and smooth muscle of the heart and prostate (1) Mature CHODL consists of an extracellular domain (ECD) containing one C-type lectin carbohydrate recognition domain (CRD), a helical transmembrane domain, and a cytoplasmic tail. The ECD of CHODL shares 94% and 95% amino acid sequence identity with mouse and rat CHODL, respectively. Multiple isoforms of CHODL with varying C-terminal deletions are generated as a result of alternative splicing (2,3). CHODL is required for proper neuromuscular junction differentiation and is tightly regulated during early embryonic development (4). Dysregulation of CHODL expression is associated with spinal muscular atrophy in mouse (3). Overexpression of CHODL is associated with enhanced cell growth and invasion in lung cancer, while silencing of CHODL in colorectal cancer is a poor prognostic factor (5,6). Expression of specific CHODL isoforms is associated with T cell maturation (2).

  1. Weng, L. et al. (2002) Genomics. 80:62.
  2. Weng, L. et al. (2003) J. Biol. Chem. 278:19164.
  3. Sleigh, J. et al. (2013) Hum. Mol. Genet. 23:855.
  4. Oprişoreanu, A.M. et al. (2019) Cell Rep. 29:1082.
  5. Masuda, K. et al. (2011) Clin. Cancer Res. 17:7712.
  6. Zhang, X. et al. (2020) J. Cancer. 11:2874.
Entrez Gene IDs
140578 (Human); 246048 (Mouse)
Alternate Names
C21orf68; CHODL; Chondrolectin; chromosome 21 open reading frame 68; FLJ12627; MT75; PRED12; Transmembrane protein MT75


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