Recombinant Human CLC/CNTFR alpha Chimera Protein, CF

Formulations:
Catalog # Availability Size / Price Qty
2415-CR-025/CF
Product Details
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Recombinant Human CLC/CNTFR alpha Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 10-25 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human CLC/CNTF R alpha Chimera protein
Human
CNTF R alpha
(Gln23 - Pro346)
Accession # P26992
GSGSSRGGSGSGGSGGGGSKL Human CLC
(Leu28 - Phe225)
Accession # Q9UBD9
6-His tag
N-terminus         C-terminus
N-terminal Sequence
Analysis
No results obtained: Gln23 predicted
Predicted Molecular Mass
61 kDa
SDS-PAGE
85-93 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2415-CR/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2415-CR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CLC/CNTF R alpha Chimera

Cardiotrophin-like cytokine (CLC), (also known as novel neurotrophin-1 (NNT-1) and B cell stimulating factor (BSF-3) is a 22 - 25 kDa member of the IL-6 family of cytokines (1 - 3). As such, it is expressed as a long, type I cytokine with four alpha -helices in its structure (2). Human CLC is synthesized as a 225 amino acid (aa) precursor that contains a 27 aa signal sequence and a 198 aa mature region. It contains one potential N-linked glycosylation site that is apparently utilized, and two distinct binding sites for Ciliary Neurotrophic Factor Receptor alpha (CNTF R alpha ) and Cytokine Receptor-like Factor (CLF) (4, 5). Although CLC has a signal sequence, it is not secreted unless noncovalently complexed to either CLF or soluble CNTF R alpha (5, 6). Once complexed, CLC signals through a tripartite receptor complex composed of gp130, LIF R beta and CNTF R alpha (5, 7). Within the IL-6 family, human CLC is most homologous to cardiotrophin-1, sharing approximately 29% amino acid sequence identity (8). Human to mouse, mature CLC shares 96% aa sequence identity. CLC is a trophic factor for motor neurons, a stimulator of ACTH release from corticotrophs, and an inducer of IgE synthesis and B cell proliferation (9 - 11). Cells known to express CLC include embryonic muscle, lung epithelium, and mesenchyme in various regions (12).

References
  1. Murakami, M. et al. (2004) Growth Factors 22:75.
  2. Ernst, M. and B.J. Jenkins (2004) Trends Genet. 20:23.
  3. Senaldi, G. et al. (1999) Proc.Natl. Acad. Sci. USA 96:11458.
  4. Perret, D. et al. (2003) J. Biol. Chem. 279:43961.
  5. Plun-Favreau, H. et al. (2001) EMBO J. 20:1692.
  6. Elson, G.C.A. et al. (2000) Nat. Neurosci. 3:867.
  7. Lelievre, E. et al. (2001) J. Biol. Chem. 276:22476.
  8. Derouet, D. et al. (2004) Proc. Natl. Acad. Sci. USA 101:4827.
  9. Forger, N.G. et al. (2003) J. Neurosci. 23:8854
  10. Auernhammer, C.J. et al. (2003) Endocrinology 144:1202.
  11. Senaldi, G. et al. (2002) J. Immunol. 168:5690.
  12. de Bovis, B. et al. (2005) Cell Commun. Signal. 3:1.
Alternate Names
CLC/CNTF R alpha Chimera

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