1 μg/lane of Recombinant Human CLEC-2A was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing R bands at 22.3, 28.4 kDa and NR bands at 21.8, 29.1 kDa.
CLEC-2A, also called keratinocyte-associated C-type lectin (KACL), is a type 2 transmembrane glycoprotein member of the C-type lectin-like receptor (CTLR) family. CLEC-A2 is part of the subgroup of CLEC-2 proteins that also includes CLEC-2B/AICL, CLEC-2D/LLT, and CD69/CLEC-2C, all of which are encoded by the natural killer gene complex (NKC) (1, 2). CLEC-2A is composed of a 126 amino acid (aa) carboxy terminal extracellular C-type lectin-like domain (CTLD), a 21 aa transmembrane domain and a 27 aa amino terminal cytoplasmic domain. The CTLD folds into a compact structure with two alpha -helices and two antiparallel beta -sheets stabilized by three conserved intramolecular disulfide bonds (3). CLEC-2 proteins act as ligands and interact with NKRP1 receptors which also are CTLR and encoded by the NKC. The crystal structure of the complex shows CLEC-2A as a non-disulfide linked homodimer that symmetrically binds two NKp65 monomers. Interaction occurs via the membrane-distal surface of the CTLD in a head-to-head orientation (4). Five key residues for CLEC-2A binding to NKp65 are conserved or conservatively substituted among CLEC-2 proteins. Thus, the CLEC-2A-NKp65 interaction is proposed as a model for all NKRP1-CLEC-2 complexes (4). Orthologs for NKp65 and CLEC-2A are present in chimpanzee, rhesus macaque and cow but have not been described in rodents (5). Whereas CLEC-2B/AICL, CLEC-2D/LLT, and CD69/CLEC-2C are generally expressed on hematopoietic cells, CLEC-2A expression is restricted to keratinocytes. In vitro, the NKp65-CLEC-2A interaction will trigger natural killer cell cytotoxicity and the release of proinflammatory cytokines (6). Thus, CLEC-2A facilitates NKp65-mediated immunosurveillance of keratinocytes (6).
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C-type Lectin Domain Family 2, Member A
Entrez Gene IDs:
CLEC2A C-type lectin domain family 2, member A ; CLEC2A; CLEC-2A; KACL; PILAR; UNQ5792
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