Recombinant Human CLEC-2A Protein, CF

R&D Systems | Catalog # 8435-CL

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human CLEC-2A Protein (8435-CL)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

Q6UVW9

Applications

Bioactivity
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Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CLEC-2A protein
Trp49-Leu174 (Gly136Asp), with an N-terminal HA tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr

Predicted Molecular Mass

16 kDa

SDS-PAGE

19-30 kDa, reducing conditions

Activity

Measured by its ability to induce IFN-gamma secretion by mouse splenocytes.
The ED50 for this effect is 0.4-2 μg/mL.

Scientific Data Images for Recombinant Human CLEC-2A Protein, CF

Recombinant Human CLEC-2A Protein Bioactivity

Recombinant Human CLEC-2A Protein Bioactivity

Recombinant Human CLEC‑2A (Catalog # 8435-CL) induces IFN-gamma secretion by mouse splenocytes. The ED50 for this effect is 0.4-2 μg/mL.
Recombinant Human CLEC-2A Protein SDS-PAGE

Recombinant Human CLEC-2A Protein SDS-PAGE

1 μg/lane of Recombinant Human CLEC-2A was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing R bands at 22.3, 28.4 kDa and NR bands at 21.8, 29.1 kDa.

Formulation, Preparation, and Storage

8435-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CLEC-2A

CLEC-2A, also called keratinocyte-associated C-type lectin (KACL), is a type 2 transmembrane glycoprotein member of the C-type lectin-like receptor (CTLR) family. CLEC-A2 is part of the subgroup of CLEC-2 proteins that also includes CLEC-2B/AICL, CLEC-2D/LLT, and CD69/CLEC-2C, all of which are encoded by the natural killer gene complex (NKC) (1, 2). CLEC-2A is composed of a 126 amino acid (aa) carboxy terminal extracellular C-type lectin-like domain (CTLD), a 21 aa transmembrane domain and a 27 aa amino terminal cytoplasmic domain. The CTLD folds into a compact structure with two alpha -helices and two antiparallel beta -sheets stabilized by three conserved intramolecular disulfide bonds (3). CLEC-2 proteins act as ligands and interact with NKRP1 receptors which also are CTLR and encoded by the NKC. The crystal structure of the complex shows CLEC-2A as a non-disulfide linked homodimer that symmetrically binds two NKp65 monomers. Interaction occurs via the membrane-distal surface of the CTLD in a head-to-head orientation (4). Five key residues for CLEC-2A binding to NKp65 are conserved or conservatively substituted among CLEC-2 proteins. Thus, the CLEC-2A-NKp65 interaction is proposed as a model for all NKRP1-CLEC-2 complexes (4). Orthologs for NKp65 and CLEC-2A are present in chimpanzee, rhesus macaque and cow but have not been described in rodents (5). Whereas CLEC-2B/AICL, CLEC-2D/LLT, and CD69/CLEC-2C are generally expressed on hematopoietic cells, CLEC-2A expression is restricted to keratinocytes. In vitro, the NKp65-CLEC-2A interaction will trigger natural killer cell cytotoxicity and the release of proinflammatory cytokines (6). Thus, CLEC-2A facilitates NKp65-mediated immunosurveillance of keratinocytes (6).

References

  1. Spreu, J. et al. (2007) Immunogenetics 59:903.
  2. Yokohama, W.M. et al. (2003) Nat.Rev.Immunol. 3:304.
  3. Zelensky, A.N. et al. (2005) FEBS J. 272:6179.
  4. Li, Y. et al. (2013) Proc. Natl. Acad. Sci. USA 110:11505.
  5. Vogler, I. et al. (2011) J. Innate Immun. 3:227.
  6. Spreu, J. et al. (2010) Proc. Natl. Acad. Sci. USA 107:5100.

Long Name

C-type Lectin Domain Family 2, Member A

Alternate Names

CLEC2A, KACL, PILAR

Entrez Gene IDs

387836 (Human)

Gene Symbol

CLEC2A

UniProt

Q6UVW9 (Human)

Additional CLEC-2A Products

Product Documents for Recombinant Human CLEC-2A Protein, CF

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Product Specific Notices for Recombinant Human CLEC-2A Protein, CF

For research use only

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