Recombinant Human CLF-1/CLC Complex Protein

Carrier Free

Catalog # Availability Size / Price Qty
1151-CL-025/CF

With Carrier

Catalog # Availability Size / Price Qty
1151-CL-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (3)
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Recombinant Human CLF-1/CLC Complex Protein Summary

Product Specifications

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells transfected with human CNTF R alpha. The ED50 for this effect is 0.5‑3.5 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human CLF-1/CLC Complex protein
Human CLF-1
(Ala38 - Arg422)
Accession # O75462.1
10-His tag
Human CLC
(Leu28 - Phe225)
Accession # Q9UBD9
6-His tag
N-terminus C-terminus
N-terminal Sequence
Analysis
Ala38 (CLF-1) & Leu28 (CLC)
Predicted Molecular Mass
44.5 kDa (CLF-1) & 23 kDa (CLC)
SDS-PAGE
50-65 kDa (CLF-1) & 28-30 kDa (CLC), reducing conditions

Product Datasheets

1151-CL (with carrier)

1151-CL/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1151-CL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1151-CL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CLF-1/CLC Complex

Cardiotrophin-like cytokine (CLC), also known as novel neurotrophin-1 (NNT-1) and B cell stimulating factor (BSF-3), is a member of the IL-6 family of cytokines (1, 2). CLC associates with the secreted soluble cytokine-like factor1 (CLF-1), a member of the Cytokine Type I Receptor family, to form the heteromeric composite cytokine CLC/CLF-1 (3, 5). CLC can also form an alternate composite cytokine with soluble ciliary neurotrophic factor receptor alpha (CNTF R alpha ) (4). Co-expression of CLC with either CLF-1 or CNTF R alpha is required for the formation of the composite cytokines and for CLC secretion (3 - 5). CLC/CLF-1 binds to the membrane-associated CNTF R alpha to initiate the heterodimerization between gp130 and leukemia inhibitory factor receptor (LIFR) and stimulate thePI 3-kinase and the MAP kinase activity. The CLC/CLF-1 complex displays activities only on those cells expressing the functional tripartite receptor complex (5). Human and mouse CLF-1 share 96% amino acid sequence identity (3). Human CLC is most homologous to cardiotrophin-1, sharing approximately 29% amino acid sequence homology. Human CLC also shares 96% amino acid sequence homology with mouse CLC. CLC/CLF-1 supports the survival of embryonic motor and sympathetic neurons and has been shown to induce astrocytes differentiation of fetal neuroepithelial cells (4 - 6). Both CLF-1 and CLC are expressed in the embryo, suggesting that the composite cytokine may have an important role in nervous system development (5, 7). CLC has also been shown to regulate immune functions by stimulating B cell proliferation and Ig production (8).

References
  1. Senaldi, G. et al. (1999) Proc.Natl. Acad. Sci. USA 96:11458.
  2. Shi, Y. et al. (1999) Biochem. Biophys. Res. Commun. 262:132.
  3. Elson, G.C.A. et al. (1998) J. Immunol. 161: 1371.
  4. Plun-Favreau, H. et al. (2001) EMBO J. 20:1692.
  5. Elson, G.C.A. et al. (2000) Nature Neurosci. 3:867.
  6. Uemura, A. et al. (2002) Cytokine 18:1.
  7. Lelievre, E. et al. (2001) J. Biol. Chem. 276:22476.
  8. Senaldi, G. et al. (2002) J. Immunol. 168:5690.
Entrez Gene IDs
9244 (Human)
Alternate Names
CLF-1/CLC Complex

Citations for Recombinant Human CLF-1/CLC Complex Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Cytokine-like factor 1 gene expression is enriched in idiopathic pulmonary fibrosis and drives the accumulation of CD4+ T cells in murine lungs: evidence for an antifibrotic role in bleomycin injury.
    Authors: Kass DJ, Yu G, Loh KS, Savir A, Borczuk A, Kahloon R, Juan-Guardela B, Deiuliis G, Tedrow J, Choi J, Richards T, Kaminski N, Greenberg SM
    Am. J. Pathol., 2012;180(5):1963-78.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Sortilin facilitates signaling of ciliary neurotrophic factor and related helical type 1 cytokines targeting the gp130/leukemia inhibitory factor receptor beta heterodimer.
    Authors: Larsen JV, Hansen M, Moller B, Madsen P, Scheller J, Nielsen M, Petersen CM
    Mol. Cell. Biol., 2010;17(0):4175.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  3. Novel regulators of kidney development from the tips of the ureteric bud.
    Authors: Schmidt-Ott KM, Yang J, Chen X, Wang H, Paragas N, Mori K, Li JY, Lu B, Costantini F, Schiffer M, Bottinger E, Barasch J
    J. Am. Soc. Nephrol., 2005;16(7):1993-2002.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay

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