Recombinant Human Complement Component C5a Protein

Formulations:
Catalog # Availability Size / Price Qty
2037-C5-025
Product Details
Citations (14)
FAQs
Reviews (1)

Recombinant Human Complement Component C5a Protein Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce N-acetyl-beta -D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. Gerard, NP and Gerard, C. (1990) Biochemistry 29:9274. The ED50 for this effect is 1.5-5 ng/mL.
Source
E. coli-derived human Complement Component C5a protein
Thr678-Arg751
Accession #
N-terminal Sequence
Analysis
Thr678
Predicted Molecular Mass
8.3 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2037-C5

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2037-C5/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: Complement Component C5a

Human Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C‑terminal arginine, creating a low activity C5adesArg74 molecule (1). Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2 (4). Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation (1, 5). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.

References
  1. Gerard, C. and N.P. Gerard, (1994) Annu. Rev. Immunol. 12:775.
  2. DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
  3. Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
  4. Okinaga, S. et al. (2003) Biochemistry 42:9406.
  5. Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.
Entrez Gene IDs
727 (Human); 15139 (Mouse)
Alternate Names
C5; C5A; Complement Component C5a; HE

Citations for Recombinant Human Complement Component C5a Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Apigenin inhibits C5a-induced proliferation of human nasopharyngeal carcinoma cells through down-regulation of C5aR
    Authors: Y Zhang, Y Cao, L Zhang, C Feng, G Zhou, G Wen
    Biosci. Rep., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Broad adsorption of sepsis-related PAMP and DAMP molecules, mycotoxins, and cytokines from whole blood using CytoSorb� sorbent porous polymer beads
    Authors: MC Gruda, KG Ruggeberg, P O'Sullivan, T Guliashvil, AR Scheirer, TD Golobish, VJ Capponi, PP Chan
    PLoS ONE, 2018;13(1):e0191676.
    Species: Human
    Sample Types: Protein
    Applications: Protein Filtration
  3. Rapid and Efficient Production of Human Functional Mast Cells Through a Three-Dimensional Culture of Adipose Tissue-Derived Stromal Vascular Cells
    Authors: J Paupert, E Espinosa, N Cenac, V Robert, P Laharrague, SM Evrard, L Casteilla, A Lorsignol, B Cousin
    J. Immunol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Tumor-associated mesenchymal stem-like cells provide extracellular signaling cue for invasiveness of glioblastoma cells
    Authors: EJ Lim, Y Suh, KC Yoo, JH Lee, IG Kim, MJ Kim, JH Chang, SG Kang, SJ Lee
    Oncotarget, 2017;8(1):1438-1448.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Nerve Growth Factor Secretion From Pulp Fibroblasts is Modulated by Complement C5a Receptor and Implied in Neurite Outgrowth
    Sci Rep, 2016;6(0):31799.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Adsorption as a Contributor for Inflammatory Mediators Removal by Different Hemofiltration Membranes: A Pilot Study
    Authors: Benjamin Malard
    Artif Organs, 2016;0(0):.
    Species: Human
    Sample Types: Plasma
    Applications: Hemofiltration Procedure
  7. Structural basis for the targeting of complement anaphylatoxin C5a using a mixed L-RNA/L-DNA aptamer.
    Authors: Yatime, Laure, Maasch, Christia, Hoehlig, Kai, Klussmann, Sven, Andersen, Gregers, Vater, Axel
    Nat Commun, 2015;6(0):6481.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Pulp fibroblasts synthesize functional complement proteins involved in initiating dentin-pulp regeneration.
    Authors: Chmilewsky F, Jeanneau C, Laurent P, About I
    Am J Pathol, 2014;184(7):1991-2000.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Molecular and stimulus-response profiles illustrate heterogeneity between peripheral and cord blood-derived human mast cells.
    Authors: Jensen B, Frandsen P, Raaby E, Schiotz P, Skov P, Poulsen L
    J Leukoc Biol, 2014;95(6):893-901.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. A novel C5a-neutralizing mirror-image (l-)aptamer prevents organ failure and improves survival in experimental sepsis.
    Authors: Hoehlig, Kai, Maasch, Christia, Shushakova, Nelli, Buchner, Klaus, Huber-Lang, Markus, Purschke, Werner G, Vater, Axel, Klussmann, Sven
    Mol Ther, 2013;21(12):2236-46.
    Species: N/A
    Sample Types: Protein
    Applications: Surface Plasmon Resonance
  11. Complement component C5a Promotes Expression of IL-22 and IL-17 from Human T cells and its Implication in Age-related Macular Degeneration.
    Authors: Liu B, Wei L, Meyerle C, Tuo J, Sen HN, Li Z, Chakrabarty S, Agron E, Chan CC, Klein ML, Chew E, Ferris F, Nussenblatt RB
    J Transl Med, 2011;9(0):111.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Complement C3a, CpG oligos, and DNA/C3a complex stimulate IFN-alpha production in a receptor for advanced glycation end product-dependent manner.
    Authors: Ruan BH, Li X, Winkler AR, Cunningham KM, Kuai J, Greco RM, Nocka KH, Fitz LJ, Wright JF, Pittman DD, Tan XY, Paulsen JE, Lin LL, Winkler DG
    J. Immunol., 2010;185(7):4213-22.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  13. CX3CL1/fractalkine is released from apoptotic lymphocytes to stimulate macrophage chemotaxis.
    Authors: Truman LA, Ford CA, Pasikowska M, Pound JD, Wilkinson SJ, Dumitriu IE, Melville L, Melrose LA, Ogden CA, Nibbs R, Graham G, Combadiere C, Gregory CD
    Blood, 2008;112(13):5026-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Functional roles for C5a receptors in sepsis.
    Authors: Rittirsch D, Flierl MA, Nadeau BA, Day DE, Huber-Lang M, Mackay CR, Zetoune FS, Gerard NP, Cianflone K, Kohl J, Gerard C, Sarma JV, Ward PA
    Nat. Med., 2008;14(5):551-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human Complement Component C5a Protein
By Anonymous on 12/01/2017
Application: In vitro bioactivity in cell culture