Recombinant Human Contactin-5 Protein, CF Summary
Glu19-Gln1059, with a C-terminal 10-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Contactin-5 (CNTN5), also known as NB-2, is a neural adhesion molecule in the contactin family of the immunoglobulin superfamily. The contactin family comprises six members (CNTN1/F3, CNTN2/TAG-1, CNTN3/BIG-1, CNTN4/BIG-2, CNTN5/NB-2 and CNTN6/NB-3) that are characterized by the presence of six Ig-like domains, four fibronectin type III-like repeats, and a glycosylphosphatidylinositol (GPI)-anchoring domain (1, 2). The human Contactin-5 cDNA exists in two splice forms which contain an 18 amino acid (aa) signal sequence and a 28 aa C-terminal propeptide. The long isoform is a 1082 aa protein that shares 91% aa sequence identity with rat and mouse Contactin-5. The short isoform lacks the first 74 aa at the N-terminus of the long isoform. Human Contactin-5 shares 43%, 41%, 52%, 52%, and 48% aa identity with Contactins-1,-2,-3,-4, and -6, respectively (3). Contactin family proteins exist as membrane-bound proteins, but can also be released as soluble proteins by GPI-specific phospholipase D. The gene encoding Contactin-5 is localized to a chromosomal region associated with schizophrenia and the neuronal disorder Jacobsen syndrome (4). The highest expression of human Contactin-5 is seen in occipital lobe and amygdala, followed by cerebral cortex, frontal lobe, thalamus, and temporal lobe (4). In rat, Contactin-5 is highly expressed specifically in structures of the central auditory pathway (5). Also in rat, Contactin-5 has been shown to promote neurite outgrowth of cerebral cortical neurons in vitro (5). Deficient Contactin-5 expression in mice results in impaired neuronal activity of the central auditory system (6).
- Kazarinova-Noyes, K. and P. Shrager (2002) Mol. Neurobiol. 26:167.
- Denisenko-Nehrbass, N. et al. (2002) J. Physiol. Paris 96:99.
- Ogawa, J. et al. (1996) Neurosci. Lett. 218:173.
- Kamei, Y. et al. (2000) Genomics 69:113.
- Ogawa, J. et al. (2001) J. Neurosci. Res. 65:100.
- Li, H. et al. (2003) Eur. J. Neurosci. 17:929.
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