Recombinant Human CRTAC1 Protein, CF

R&D Systems | Catalog # 5234-CR

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human CRTAC1 Protein (5234-CR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q9NQ79

Structure / Form

Monomer

Applications

Bioactivity
View all CRTAC1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CRTAC1 protein
Ser28-Cys661, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser28

Predicted Molecular Mass

69 kDa

SDS-PAGE

83-90 kDa, reducing conditions

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.
The ED50 for this effect is 0.25-1.5 μg/mL.

Reviewed Applications

Read 1 review rated 4 using 5234-CR in the following applications:

Formulation, Preparation, and Storage

5234-CR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CRTAC1

Cartilage acidic protein 1 (CRTAC1), also known as CEP‑68, is a 95 kDa glycosylated protein that contains four FG‑GAP domains and one EGF‑like domain. An integrin‑binding RGD motif is present in the human protein but absent in the mouse and rat proteins. Mature human CRTAC1 shares 89% amino acid sequence identity with mouse and rat CRTAC. It is secreted by chondrocytes into the extracellular matrix of cartilage (1, 2). It is also up‑regulated in type II alveolar epithelial cells upon dexamethasone exposure (3). CRTAC1B, otherwise known as LOTUS, is an 85 kDa alternatively spliced isoform that has a substituted C‑terminal region with a potential transmembrane segment (2, 4). CRTAC1B is preferentially expressed in select regions of the brain on neuronal growth cones and neurite shafts (2, 4). It associates with the Nogo Receptor (NgR) and prevents Nogo‑induced growth cone collapse (4, 5). In mouse, CRTAC1B plays an important role in axonal bundling and formation of the lateral olfactory tract (4).

References

  1. Steck, E. et al. (2001) Biochem. J. 353:169.
  2. Steck, E. et al. (2007) Matrix Biol. 26:30.
  3. Ballard, P.L. et al. (2010) Am. J. Physiol. Lung Cell. Mol. Physiol. 299:L36.
  4. Sato, Y. et al. (2011) Science 333:769.
  5. Kurihara, Y. et al. (2012) Biochem. Biophys. Res. Commun. 418:390.

Long Name

Cartilage Acidic Protein 1

Alternate Names

ASPIC1, CEP68

Entrez Gene IDs

55118 (Human); 72832 (Mouse); 171438 (Rat)

Gene Symbol

CRTAC1

UniProt

Q9NQ79

Additional CRTAC1 Products

Product Documents for Recombinant Human CRTAC1 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human CRTAC1 Protein, CF

For research use only

Citations for Recombinant Human CRTAC1 Protein, CF

Customer Reviews for Recombinant Human CRTAC1 Protein, CF (1)

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    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 05/17/2016

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