Recombinant Human CUL2/RBX1 Complex Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human CUL2/RBX1 Complex Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Typical enzyme concentration to support in vitro conjugation will depend on experimental conditions.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL2/RBX1 Complex protein
Met1-Ala 745 with a N-terminal 10-His Sumo tag (CUL2), Met1-His108 (RBX1)
Predicted Molecular Mass
93 kDa (CUL2), 12 kDa (RBX1)

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Background: CUL2/RBX1 Complex

Cullin-2 is a core component of multiple cullin-RING based "ECS" (Elongin B/C-CUL2-SOCS-box protein) E3 Ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in a variety of cellular processes.  In the ECS complex, Cullin-2 serves as a scaffold that organizes the ELOB/C-associated recognition subunits with the RBX1 subunit and contributes to the catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  In vivo, the E3 ubiquitin ligase activity of the ECS complex is dependent on neddylation of the cullin subunit, though neddylation may be dispensable for some in vitro reactions. 

  1. Baek K. et al. (2020) Nature 578: 461.
  2. Duda D.M. et al. (2008) Cell  134: 995.
  3. Duda D.M. et al. (2012) Mol. Cell  47: 371.
  4. Goldenberg S.J. et al. (2004) Cell  119: 517.
  5. Zheng N. et al. (2002) Nature  416: 703.
Long Name
Cullin-2/RING Box Protein 1 Complex
Entrez Gene IDs
8453 (Human)
Alternate Names
CUL2/RBX1 Complex

Citation for Recombinant Human CUL2/RBX1 Complex Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Expression and purification of functional recombinant CUL2�RBX1 from E. coli
    Authors: S Diaz, L Li, K Wang, X Liu
    Scientific Reports, 2021-05-27;11(1):11224.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay


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