Recombinant Human CUL3/RBX2 Complex His-tag Protein, CF

R&D Systems | Catalog # E3-430

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant Human CUL3/RBX2 Complex His-tag Protein (E3-430)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession Number

Q13618.2 (CUL3), Q9UBF6.1 (RBX2)

Applications

Bioactivity
View all CUL3/RBX2 Complex Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL3/RBX2 Complex protein
Met1 - Ala768 with a N-terminal 10-His tag (CUL3), Met1 - Lys113 (RBX2)

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

92 kDa (CUL3), 13 kDa (RBX2)

Activity

Typical enzyme concentration to support in vitro conjugation will depend on experimental conditions.

Formulation, Preparation, and Storage

E3-430
Formulation Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: CUL3/RBX2 Complex

Cullin-3 (CUL3) is a core component of multiple BCR (BTB-CUL3-RBX) E3 Ubiquitin ligase complexes that mediate the ubiquitination of several classes of signaling and structural proteins.  In the BCR complex, CUL3 serves as a scaffold that organizes one or more BTB (BR-C, Ttk and Bab, also known as a POZ domain) substrate recognition subunits with the RBX subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  Substrate specificity of a BCR ligase is determined by the BTB domain protein(s) associated with the ligase, though BTB-independent CUL3 ligase activity has been reported.  In vivo, the E3 ubiquitin ligase of the BCR complex is dependent on neddylation of the cullin subunit, though neddylation may be dispensable for some in vitro reactions.  

References

  1. Baek K., et al. (2020) Nature  578: 461.
  2. Choo Y.Y & Hagen T. (2012) PLoS One  7: e41350.
  3. Davidge B., et al. (2019) J. Cell Sci.  132: jcs233049.
  4. Duda D.M., et al. (2012) Mol. Cell  47: 371.
  5. Stogios P.J. et al. (2005) Genome Biol.  6: R82.

Long Name

Cullin 3/Ring Box Protein 2

UniProt

Q13618.2 (CUL3), Q9UBF6.1 (RBX2)

Additional CUL3/RBX2 Complex Products

Product Documents for Recombinant Human CUL3/RBX2 Complex His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human CUL3/RBX2 Complex His-tag Protein, CF

For research use only

Customer Reviews for Recombinant Human CUL3/RBX2 Complex His-tag Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human CUL3/RBX2 Complex His-tag Protein, CF and earn rewards!

Have you used Recombinant Human CUL3/RBX2 Complex His-tag Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes