Recombinant Human CUL4A/RBX1 Complex Protein, CF

R&D Systems | Catalog # E3-440

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant Human CUL4A/RBX1 Complex Protein (E3-440)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession Number

Q13619.3 (CUL4A), P62877.1 (RBX1)

Applications

Bioactivity
View all CUL4A/RBX1 Complex Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL4A/RBX1 Complex protein
Met1 - Ala759 with a N-terminal 10-His Sumo tag (CUL4A), Met1 - His108 (RBX1)

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

94 kDa (CUL4A), 12 kDa (RBX1)

Activity

Typical enzyme concentration to support in vitro conjugation will depend on experimental conditions.

Formulation, Preparation, and Storage

E3-440
Formulation Supplied as a solution in HEPES, NaCl, Glycerol and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: CUL4A/RBX1 Complex

Cullin-4A (CUL4A) is a core component of multiple cullin-RING type E3 Ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in cell cycle progression, DNA repair and other processes.  In the DCX complex (DDB1-CUL4-X-box) CUL4A serves as a scaffold that organizes the DDB1-X-box recognition subunits with the RBX1 subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  In vivo, the E3 ubiquitin ligase activity of the DCX complex is dependent on neddylation of the cullin subunit, though neddylation may be dispensable for some in vitro reactions.  

References

  1. Baek K., et al. (2020 Nature 578: 461.
  2. Fischer E.S., et al.  (2014) Nature  512: 49.
  3. He Y.J., et al.  (2006) Genes Dev.  20: 2949.
  4. Ito T., et al.  (2010) Science  327: 1345.
  5. Wang H., et al.  (2006) Mol. Cell  22: 383.

Long Name

Cullin-4A/RING Box Protein 1 Complex

Entrez Gene IDs

8451 (Human)

Gene Symbol

CUL4A

UniProt

Q13619.3 (CUL4A), P62877.1 (RBX1)

Additional CUL4A/RBX1 Complex Products

Product Documents for Recombinant Human CUL4A/RBX1 Complex Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human CUL4A/RBX1 Complex Protein, CF

For research use only

Customer Reviews for Recombinant Human CUL4A/RBX1 Complex Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human CUL4A/RBX1 Complex Protein, CF and earn rewards!

Have you used Recombinant Human CUL4A/RBX1 Complex Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes