Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Product Details

Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF Summary

Product Specifications

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Typical concentration to support in vitro conjugation reactions will depend on experimental conditions.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL4A/RBX1/DDB1/CRBN Complex protein
This complex consists of an N-terminal 10-His tagged Cullin-4A, untagged RBX1, untagged DDB1 and untagged CRBN.
Predicted Molecular Mass
94 kDa (CUL4A), 12 kDa (RBX1), 127 kDa (DDB1), 51 kDa (CRBN)

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Background: CUL4A/RBX1/DDB1/CRBN Complex

Cullin-4A (CUL4A) is a core component of multiple cullin-RING type Ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in cell cycle progression, DNA repair and other processes.  In the DCX complex (DDB1-CUL4-X-box), CUL4A serves as a scaffold that organizes the DDB1-X-box substrate recognition subunits with the RBX1 subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  In vivo, the E3 ubiquitin ligase activity of the DCX complex is dependent on neddylation of the CUL4 subunit, though neddylation may be dispensable in some in vitro reactions.  

  1. Fischer E.S., et al.  (2014) Nature  512: 49.
  2. He Y.J., et al.  (2006) Genes Dev.  20: 2949.
  3. Ito T., et al.  (2010) Science  327: 1345.
  4. Wang H., et al.  (2006) Mol. Cell  22: 383.
Long Name
Cullin-4A/RING Box Protein 1/Damage Specific DNA Binding Protein 1/Cereblon Complex
Alternate Names


  1. Can I purchase CRBN separately?

    • At this time, CRBN is only available as a complex. If you are interested in a custom, stand-alone CRBN protein, please contact Custom Services (

  2. What is the main difference between the Recombinant Human CUL4A/NEDD8/RBX1/DDB1/CRBN Protein, CF (Catalog # E3-651) and the Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF (Catalog # E3-650)?

    • The main difference between these products is that Catalog # E3-651 includes NEDD8 in the complex with CUL4A, RBX1, DDB1, and CRBN. CUL4A is neddylated, or attached to NEDD8. Catalog # E3-650 contains all of the same proteins in a complex (except NEDD8), but CUL4A is not neddylated.

      For this complex, neddylation is required for ligase activity in vivo, but generally not for in vitro activity. For in vitro applications, both complexes should work similarly and Catalog # E3-651 should be able to perform all of the functions of Catalog # E3-650.

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