Recombinant Human CXADR Fc Chimera Protein, CF

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Recombinant Human CXADR Fc Chimera Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. Recombinant Human CXADR Fc Chimera (Catalog # 3336-CX) binds Recombinant Human AMICA/JAML Fc Chimera (Catalog # 3449-AM) with an ED50 of 2.50-25.0 ng/mL.
Mouse myeloma cell line, NS0-derived human CXADR protein
(Leu20 - Gly237)
Accession # P78310
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
50.6 kDa (monomer)
60-65 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CXADR

CXADR (coxsackie and adenovirus receptor), also known as CAR, is a 46 kDa type I transmembrane glycoprotein that belongs to the CTX family of the Ig superfamily (1 - 3). CXADR has received attention as a receptor that facilitates gene transfer mediated by most adenoviruses (1, 2). It is also an adhesion molecule within junctional complexes, notably between epithelial cells lining body cavities and within myocardial intercalated discs (1, 2, 4). CXADR is essential for normal cardiac development in the mouse (7). It is expressed throughout brain neuroepithelium during development, but mainly in ependymal cells in the adult (4 - 6). The 365 amino acid (aa) human CXADR contains a 19 aa signal sequence, a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 107 aa intracellular domain. D1 is thought to be responsible for homodimer formation in trans within tight junctions (2). The fiber knob of adenoviruses attaches at a similar site, and evidence suggests that disruption of tight junctions facilitates virus binding (1, 2). The C-terminus interacts with several cytoplasmic junctional proteins, microtubules and the actin cytoskeleton (1, 8, 9). CXADR interaction with junctional adhesion molecule-like protein (JAML) has been shown to have important functional roles in immunity, inflammation, and tissue homeostatsis (10).The ECD of human CXADR shares 90% aa sequence identity with mouse, rat, and porcine CXADR, and 92% and 89% aa identity with bovine and canine CXADR, respectively. An alternately spliced isoform (CXADR2) that diverges in the C-terminal 15 aa shows a similar expression pattern (4, 11). Transcription of splice variants that produce soluble forms of CXADR has been detected, and secreted forms in serum and pleural fluid potentially block viral infection (12).

  1. Coyne, C.B. and J.M. Bergelson (2005) Adv. Drug Deliv. Rev. 57:869.
  2. Philipson, L. and R.F. Pettersson (2004) Curr. Top. Microbiol. Immunol. 273:87.
  3. Tomko, R.P. et al. (1997) Proc. Natl. Acad. Sci. USA 94:3352.
  4. Raschperger, E. et al. (2006) Exp. Cell Res. 312:1566.
  5. Hotta, Y. et al. Dev. Brain Res. 143:1.
  6. Hauwel, M. et al. (2005) Brain Res. Rev. 48:265.
  7. Chen, J. et al. (2006) Circ. Res. 98:923.
  8. Fok, P.T. et al. (2007) J. Biol. Chem. 282:7512.
  9. Huang, K-C. et al. (2007) FEBS Lett. 581:2702.
  10. Verdino, P. et al. Science (2010) Science 329:1210.
  11. Mirza, M. et al. (2006) Exp. Cell Res. 312:817.
  12. Bernal, R.M. et al. (2002) Clin. Cancer Res. 8:1915.
Long Name
Coxsackie and Adenovirus Receptor
Entrez Gene IDs
1525 (Human); 13052 (Mouse)
Alternate Names
CAR10Coxsackievirus B-adenovirus receptor; CAR4/6; coxsackie virus and adenovirus receptor; coxsackie virus B receptor; coxsackievirus and adenovirus receptor; CVB3 binding protein; CVB3 BP; CVB3-binding protein; CXADR; HCAR; HCVADR

Citations for Recombinant Human CXADR Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Nitric oxide suppression by secreted frizzled-related protein 2 drives retinoblastoma
    Authors: P Jayabal, F Zhou, X Ma, KM Bondra, B Blackman, ST Weintraub, Y Chen, P Chévez-Bar, PJ Houghton, B Gallie, Y Shiio
    Cell Reports, 2023-02-10;42(2):112103.
    Species: N/A
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  2. JAML immunotherapy targets recently activated tumor-infiltrating CD8+ T�cells
    Authors: S Eschweiler, A Wang, C Ramírez-Su, A von Witzle, Y Li, SJ Chee, H Simon, M Mondal, M Ellis, GJ Thomas, V Chandra, CH Ottensmeie, P Vijayanand
    Cell Reports, 2023-01-25;42(2):112040.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Heparan Sulfate Is a Cellular Receptor for Enteric Human Adenoviruses
    Authors: A Rajan, E Palm, F Trulsson, S Mundigl, M Becker, BD Persson, L Frängsmyr, A Lenman
    Viruses, 2021-02-14;13(2):.
    Species: Human
    Sample Types: Virus
    Applications: Surface Plasmon Resonance
  4. Diversity within the adenovirus fiber knob hypervariable loops influences primary receptor interactions
    Authors: AT Baker, A Greenshiel, L Coughlan, JA Davies, H Uusi-Kertt, DK Cole, PJ Rizkallah, AL Parker
    Nat Commun, 2019-02-14;10(1):741.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  5. Human adenovirus 52 uses sialic acid-containing glycoproteins and the coxsackie and adenovirus receptor for binding to target cells.
    Authors: Lenman A, Liaci A, Liu Y, Ardahl C, Rajan A, Nilsson E, Bradford W, Kaeshammer L, Jones M, Frangsmyr L, Feizi T, Stehle T, Arnberg N
    PLoS Pathog, 2015-02-12;11(2):e1004657.
    Applications: Bioassay
  6. Gene-trap mutagenesis identifies mammalian genes contributing to intoxication by Clostridium perfringens epsilon-toxin.
    Authors: Ivie SE, Fennessey CM, Sheng J, Rubin DH, McClain MS
    PLoS ONE, 2011-03-11;6(3):e17787.
    Applications: Bioassay


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Reviews for Recombinant Human CXADR Fc Chimera Protein, CF

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By Anonymous on 03/10/2021
Application: Binding assay/Protein-protein interaction

By Anonymous on 11/16/2020
Application: Cell Adhesion

Recombinant Human CXADR Fc Chimera Protein, CF
By Anonymous on 12/13/2019
Application: Binding ELISA
Reason for Rating: JAML binding detected via ELISA