Recombinant Human CXADR Fc Chimera Protein, CF

R&D Systems | Catalog # 3336-CX

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human CXADR Fc Chimera Protein (3336-CX)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P78310

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all CXADR Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CXADR protein
Human CXADR
(Leu20 - Gly237)
Accession # P78310		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu20

Predicted Molecular Mass

50.6 kDa (monomer)

SDS-PAGE

60-65 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human CXADR Fc Chimera (Catalog # 3336-CX) binds Recombinant Human AMICA/JAML Fc Chimera (Catalog # 3449-AM) with an ED50 of 2.50-25.0 ng/mL.

Reviewed Applications

Read 3 reviews rated 5 using 3336-CX in the following applications:

Formulation, Preparation, and Storage

3336-CX
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXADR

CXADR (coxsackie and adenovirus receptor), also known as CAR, is a 46 kDa type I transmembrane glycoprotein that belongs to the CTX family of the Ig superfamily (1 - 3). CXADR has received attention as a receptor that facilitates gene transfer mediated by most adenoviruses (1, 2). It is also an adhesion molecule within junctional complexes, notably between epithelial cells lining body cavities and within myocardial intercalated discs (1, 2, 4). CXADR is essential for normal cardiac development in the mouse (7). It is expressed throughout brain neuroepithelium during development, but mainly in ependymal cells in the adult (4 - 6). The 365 amino acid (aa) human CXADR contains a 19 aa signal sequence, a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 107 aa intracellular domain. D1 is thought to be responsible for homodimer formation in trans within tight junctions (2). The fiber knob of adenoviruses attaches at a similar site, and evidence suggests that disruption of tight junctions facilitates virus binding (1, 2). The C-terminus interacts with several cytoplasmic junctional proteins, microtubules and the actin cytoskeleton (1, 8, 9). CXADR interaction with junctional adhesion molecule-like protein (JAML) has been shown to have important functional roles in immunity, inflammation, and tissue homeostatsis (10).The ECD of human CXADR shares 90% aa sequence identity with mouse, rat, and porcine CXADR, and 92% and 89% aa identity with bovine and canine CXADR, respectively. An alternately spliced isoform (CXADR2) that diverges in the C-terminal 15 aa shows a similar expression pattern (4, 11). Transcription of splice variants that produce soluble forms of CXADR has been detected, and secreted forms in serum and pleural fluid potentially block viral infection (12).

References

  1. Coyne, C.B. and J.M. Bergelson (2005) Adv. Drug Deliv. Rev. 57:869.
  2. Philipson, L. and R.F. Pettersson (2004) Curr. Top. Microbiol. Immunol. 273:87.
  3. Tomko, R.P. et al. (1997) Proc. Natl. Acad. Sci. USA 94:3352.
  4. Raschperger, E. et al. (2006) Exp. Cell Res. 312:1566.
  5. Hotta, Y. et al. Dev. Brain Res. 143:1.
  6. Hauwel, M. et al. (2005) Brain Res. Rev. 48:265.
  7. Chen, J. et al. (2006) Circ. Res. 98:923.
  8. Fok, P.T. et al. (2007) J. Biol. Chem. 282:7512.
  9. Huang, K-C. et al. (2007) FEBS Lett. 581:2702.
  10. Verdino, P. et al. Science (2010) Science 329:1210.
  11. Mirza, M. et al. (2006) Exp. Cell Res. 312:817.
  12. Bernal, R.M. et al. (2002) Clin. Cancer Res. 8:1915.

Long Name

Coxsackie and Adenovirus Receptor

Alternate Names

CVB3 BP

Entrez Gene IDs

1525 (Human); 13052 (Mouse)

Gene Symbol

CXADR

UniProt

P78310

Additional CXADR Products

Product Documents for Recombinant Human CXADR Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human CXADR Fc Chimera Protein, CF

For research use only

Related Research Areas

Infectious Diseases

Citations for Recombinant Human CXADR Fc Chimera Protein, CF

Customer Reviews for Recombinant Human CXADR Fc Chimera Protein, CF (3)

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  • Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 03/10/2021
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    Application: Cell Adhesion
    Verified Customer | Posted 11/16/2020
  • Recombinant Human CXADR Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding ELISA
    Verified Customer | Posted 12/13/2019
    Recombinant Human CXADR Fc Chimera Protein, CF 3336-CX

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