Recombinant Human DAN Fc Chimera Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human DAN Fc Chimera Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit BMP-4-induced activity in MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 0.2-1 µg/mL in the presence of 50 ng/mL of rhBMP-4.
Mouse myeloma cell line, NS0-derived human DAN protein
Human DAN
Ala17 - Asp180 (Ala36Arg)
Accession # P41271.1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
44.3 kDa (monomer)
55-65 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: DAN

DAN (differential screening-selected gene aberrative in neuroblastoma) was initially identified as a gene whose expression is downregulated in src-transformed rat fibroblasts. Human DAN was isolated from a normal lung cDNA library using mouse DAN as a probe. DAN has now been shown to be a prototypical member of the DAN family of secreted glycoproteins that are putative antagonists for TGF-beta superfamily proteins. DAN family members share a cysteine-rich domain that is structurally related to the cysteine-knot motif found in TGF-beta superfamily ligands. There are at least five mammalian DAN family members including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante and PRDC (protein related to DAN and cereberus). Additional DAN family members include Xenopus Cerberus, chick Caronte and C. elegans CeCan 1. The DAN family of proteins are thought to act as antagonists by binding TGF-beta family ligands and preventing their interactions with signaling receptor complexes. Recombinant human DAN preparations from R&D Systems have been shown to bind BMP-4 in a functional ELISA and to inhibit BMP-4 mediated bioactivity in ATDC 5 chrondrogenic cells. It is likely the various DAN family members and other TGF-beta BMP antagonists including Noggin, Chordin, Follistatin and TSG can selectively antagonize the activities of different subsets of TGF-beta superfamily ligands. These antagonists represent one of the many elaborate regulatory mechanisms that have evolved to control the bioactivities of the TGF-beta superfamily ligands.

  1. Massage, J. and Y-G. Chen (2000) Genes & Development 14:627.
  2. Perch, J.J.H. et al. (1999) Develop. Biol. 209:98.
  3. Enomoto, H. et al. (1994) Oncogene 9:2785.
Long Name
Differential Screening-selected Gene Aberrant in Neuroblastoma
Entrez Gene IDs
4681 (Human); 17965 (Mouse)
Alternate Names
DAN domain family member 1; DAN; DAND1; MGC8972; NBL1; neuroblastoma suppressor of tumorigenicity 1; neuroblastoma, suppression of tumorigenicity 1; Protein N03; suppression of tumorigenicity 1; Zinc finger protein DAN

Citation for Recombinant Human DAN Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. DAN (NBL1) promotes collective neural crest migration by restraining uncontrolled invasion
    Authors: R McLennan, CM Bailey, LJ Schumacher, JM Teddy, JA Morrison, JC Kasemeier-, LA Wolfe, MM Gogol, RE Baker, PK Maini, PM Kulesa
    J. Cell Biol., 2017;216(10):3339-3354.
    Species: Chicken
    Sample Types: In Vivo
    Applications: In Vivo


  1. What is the purpose of the single amino acid substitution of Alanine for Arganine at position 36 (Ala36Arg)?

    • The Ala36Arg mutation in the sequence of the Recombinant Human DAN Fc Chimera Protein, CF (Catalog # 955-DA) does not have any functional purpose. We do not expect this mutation to have any impact on the performance of this protein.

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