Recombinant Human DDB1/CRBN Complex Protein, CF Summary
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v), Glycerol, 1 mM DTT
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: DDB1/CRBN Complex
Cereblon (CRBN) is the substrate recognition component of DCX (DDB1-CUL4-X-box) E3 Ubiquitin ligase complexes that mediate the ubiquitination of numerous target proteins including the transcriptional regulator MEIS2. CRBN plays an important role in limb outgrowth, and its function in this process is perturbed by the binding of thalidomide and related compounds. Binding of CRBN to Cullin-4 is mediated in part by DNA damage-binding protein 1 (DDB1), a component of multiple DCX class ligases. In addition to its role in limb development, DDB1 is also involved in various DNA damage response mechanisms. This product consists of untagged DDB1 (UniProt Q16531) and untagged CRBN (UniProt Q96SW2).
- Fischer E.S. et al. (2014) Nature 512: 49
- He Y.J., et al. (2006) Genes Dev. 20: 2949
- Ito T., et al. (2010) Science 327: 1345
- Wang H., et al. (2006) Mol. Cell 22: 383
Can I purchase CRBN separately?
At this time, CRBN is only available as a complex. If you are interested in a custom, stand-alone CRBN protein, please contact Custom Services (https://www.rndsystems.com/services).
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