Recombinant Human DDB1/CRBN Complex Protein, CF

Catalog # Availability Size / Price Qty
E3-500-025
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human DDB1/CRBN Complex Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Typical concentration to support in vitro applications will depend on experimental conditions.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human DDB1/CRBN Complex protein
Accession #
Predicted Molecular Mass
127 kDa (DDB1), 51 kDa (CRBN)

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-500

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v), Glycerol, 1 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 36 months from date of receipt, -70 °C as supplied.
  • 6 months, -20 to -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DDB1/CRBN Complex

Cereblon (CRBN) is the substrate recognition component of DCX (DDB1-CUL4-X-box) E3 Ubiquitin ligase complexes that mediate the ubiquitination of numerous target proteins including the transcriptional regulator MEIS2.  CRBN plays an important role in limb outgrowth, and its function in this process is perturbed by the binding of thalidomide and related compounds.  Binding of CRBN to Cullin-4 is mediated in part by DNA damage-binding protein 1 (DDB1), a component of multiple DCX class ligases.  In addition to its role in limb development, DDB1 is also involved in various DNA damage response mechanisms.  This product consists of untagged DDB1 (UniProt Q16531) and untagged CRBN (UniProt Q96SW2).

References
  1. Fischer E.S. et al. (2014) Nature  512: 49
  2. He Y.J., et al. (2006)  Genes Dev.  20: 2949
  3. Ito T., et al. (2010) Science  327: 1345
  4. Wang H., et al. (2006) Mol. Cell  22: 383
Long Name
Damage-specific DNA Binding Protein 1/Cereblon Complex
Entrez Gene IDs
51185 (Human); 58799 (Mouse); 297498 (Rat)
Alternate Names
DDB1/CRBN Complex

FAQs

  1. Can I purchase CRBN separately?

    • At this time, CRBN is only available as a complex. If you are interested in a custom, stand-alone CRBN protein, please contact Custom Services (https://www.rndsystems.com/services).

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