Recombinant Human DDB1/CRBN Complex Protein, CF

R&D Systems | Catalog # E3-500

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Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant Human DDB1/CRBN Complex Protein (E3-500)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession Number

Q16531.1, Q96SW2.1

Applications

Bioactivity
View all DDB1/CRBN Complex Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human DDB1/CRBN Complex protein

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

127 kDa (DDB1), 51 kDa (CRBN)

Activity

Typical concentration to support in vitro applications will depend on experimental conditions.

Formulation, Preparation, and Storage

E3-500
Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: DDB1/CRBN Complex

Cereblon (CRBN) is the substrate recognition component of DCX (DDB1-CUL4-X-box) E3 Ubiquitin ligase complexes that mediate the ubiquitination of numerous target proteins including the transcriptional regulator MEIS2.  CRBN plays an important role in limb outgrowth, and its function in this process is perturbed by the binding of thalidomide and related compounds.  Binding of CRBN to Cullin-4 is mediated in part by DNA damage-binding protein 1 (DDB1), a component of multiple DCX class ligases.  In addition to its role in limb development, DDB1 is also involved in various DNA damage response mechanisms.  This product consists of an untagged complex of DDB1 and CRBN.

References

  1. Fischer E.S.,et al. (2014) Nature  512: 49
  2. He Y.J., et al. (2006)  Genes Dev.  20: 2949
  3. Ito T., et al. (2010) Science  327: 1345
  4. Wang H., et al. (2006) Mol. Cell  22: 383

Long Name

Damage-specific DNA Binding Protein 1/Cereblon Complex

Entrez Gene IDs

51185 (Human); 58799 (Mouse); 297498 (Rat)

Gene Symbol

CRBN

UniProt

Q16531.1, Q96SW2.1

Additional DDB1/CRBN Complex Products

Product Documents for Recombinant Human DDB1/CRBN Complex Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human DDB1/CRBN Complex Protein, CF

For research use only

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Citations for Recombinant Human DDB1/CRBN Complex Protein, CF

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FAQs for Recombinant Human DDB1/CRBN Complex Protein, CF

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  • Q: Can I purchase CRBN separately?

    A: At this time, CRBN is only available as a complex. If you are interested in a custom, stand-alone CRBN protein, please contact Custom Services (https://www.rndsystems.com/services).

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