Recombinant Human DDR1 Fc Chimera Protein, CF

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Recombinant Human DDR1 Fc Chimera Protein, CF Summary

Product Specifications

>85%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Measured by its ability to bind Collagen I in a functional ELISA. Leitinger, B. (2003) J. Biol. Chem. 278:16761. Immobilized Collagen I at 10 µg/mL (100 µL/well) can bind rhDDR1 with an apparent KD <10 nM.
Optimal dilutions should be determined by each laboratory for each application.
Mouse myeloma cell line, NS0-derived human DDR1 protein
Human DDR1
(Asp21 - Thr416)
Accession # Q5ST11
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
70.5 kDa (monomer)
90-95 kDa, reducing conditions

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: DDR1

DDR1, also known as CAK, CD167a, RTK6, and TrkE, is a 120 - 140 kDa type I transmembrane glycoprotein that belongs to the discoidin-like domain containing subfamily of receptor tyrosine kinases (1, 2). Mature human DDR2 consists of a 398 amino acid (aa) extracellular domain (ECD) that includes the discoidin-like domain, a 27 aa transmembrane segment, and a 470 aa cytoplasmic region with a tyrosine kinase domain (3). Within the ECD, human DDR1 shares 53% aa sequence identity with human DDR2 and 93% with mouse and rat DDR1. DDR1 is expressed on epithelial tissues, activated monocytes and neutrophils, and in several cancers (2, 4). Compared to isoform DDR1b, DDR1a lacks 37 aa’s that include a Shc-interacting NPxY motif in the cytoplasmic juxtamembrane region (5). Two additional kinase deficient splice forms are expressed in colon cancer (6). The discoidin-like domain mediates binding to collagens I - V (1, 7, 8). DDR1 selectively recognizes the triple helical structure of collagen (7, 8). It is expressed on the cell surface as a dimer which can include different isoforms (5, 9). DDR1 oligomerization enhances collagen binding and also modulates collagen fibrillogenesis (10, 11). The transmembrane segment contains a leucine zipper and GxxxG motif, but neither is exclusively required for dimerization (9). Collagen binding induces prolonged autophosphorylation, including the NPxY motif (7, 8). Collagen binding also results in the proteolytic cleavage of a tyrosine phosphorylated 60 kDa C-terminal fragment (CTF), and a 60 kDa ECD fragment (12, 13). TIMP3 and TAPI-1 inhibit shedding of the ECD fragment but not the CTF (12). Overexpression of DDR1a promotes MMP-2 activation and results in an increased invasiveness of a glioblastoma cell line; DDR1b does not (14).

  1. Vogel, W.F. et al. (2006) Cell. Signal. 18:1108.
  2. Yoshimura, T. et al. (2005) Immunologic Res. 31:219.
  3. Perez, J.L. et al. (1994) Oncogene 9:211.
  4. Laval, S. et al. (1994) Cell Growth Differ. 5:1173.
  5. Perez, J.L. et al. (1996) Oncogene 12:1469.
  6. Alves, F. et al. (2001) FASEB J. 15:1321.
  7. Shrivastava, A. et al. (1997) Mol. Cell 1:25.
  8. Vogel, W. et al. (1997) Mol. Cell 1:13.
  9. Nordeen, N.A. et al. (2006) J. Biol. Chem. 281:22744.
  10. Leitinger, B. (2003) J. Biol. Chem. 278:16761.
  11. Agarwal, G. et al. (2007) J. Mol. Biol. 367:443.
  12. Slack, B.E. et al. (2006) J. Cell Biochem. 98:672.
  13. Vogel, W.F. et al. (2001) FEBS Lett. 514:175.
  14. Ram, R. et al. (2006) J. Neurooncol. 76:239.
Long Name
Discoidin Domain Receptor 1
Entrez Gene IDs
780 (Human)
Alternate Names
CAK; CD167 antigen-like family member A; CD167a antigen; CD167a; DDR; DDR1; discoidin domain receptor family, member 1; discoidin domain receptor tyrosine kinase 1; Discoidin receptor tyrosine kinase; EC 2.7.10; EC; EDDR1; ENTRK4; Epithelial discoidin domain receptor 1; HGK2; MCK10; MCK-10; NTRK4; Protein-tyrosine kinase 3A; PTK3A protein tyrosine kinase 3A; PTK3A; receptor, type 4; RTK6; TRK E; TrkE

Citations for Recombinant Human DDR1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Matrix stiffness exacerbates the proinflammatory responses of vascular smooth muscle cell through the DDR1-DNMT1 mechanotransduction axis
    Authors: J Wang, SA Xie, N Li, T Zhang, W Yao, H Zhao, W Pang, L Han, J Liu, J Zhou
    Bioactive materials, 2022;17(0):406-424.
    Species: Human
    Sample Types: Peptides
    Applications: Bioassay
  2. Selective pharmacological inhibition of DDR1 prevents experimentally-induced glomerulonephritis in prevention and therapeutic regime
    Authors: S Moll, Y Yasui, A Abed, T Murata, H Shimada, A Maeda, N Fukushima, M Kanamori, S Uhles, L Badi, T Cagarelli, I Formentini, F Drawnel, G Georges, T Bergauer, R Gasser, RD Bonfil, R Fridman, H Richter, J Funk, MJ Moeller, C Chatzianto, M Prunotto
    J Transl Med, 2018;16(1):148.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Antibody Production
  3. Collagen signaling enhances tumor progression after anti-VEGF therapy in a murine model of pancreatic ductal adenocarcinoma.
    Authors: Aguilera K, Rivera L, Hur H, Carbon J, Toombs J, Goldstein C, Dellinger M, Castrillon D, Brekken R
    Cancer Res, 2014;74(4):1032-44.
    Species: Rat
    Sample Types: Protein
    Applications: ELISA Developmet
  4. Implication of discoidin domain receptor 1 in T cell migration in three-dimensional collagen.
    Authors: Hachehouche LN, Chetoui N, Aoudjit F
    Mol. Immunol., 2010;47(9):1866-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay


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