Recombinant Human DDR1 Fc Chimera Protein, CF Summary
Optimal dilutions should be determined by each laboratory for each application.
(Asp21 - Thr416)
Accession # Q5ST11
(Pro100 - Lys330)
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
DDR1, also known as CAK, CD167a, RTK6, and TrkE, is a 120 - 140 kDa type I transmembrane glycoprotein that belongs to the discoidin-like domain containing subfamily of receptor tyrosine kinases (1, 2). Mature human DDR2 consists of a 398 amino acid (aa) extracellular domain (ECD) that includes the discoidin-like domain, a 27 aa transmembrane segment, and a 470 aa cytoplasmic region with a tyrosine kinase domain (3). Within the ECD, human DDR1 shares 53% aa sequence identity with human DDR2 and 93% with mouse and rat DDR1. DDR1 is expressed on epithelial tissues, activated monocytes and neutrophils, and in several cancers (2, 4). Compared to isoform DDR1b, DDR1a lacks 37 aa’s that include a Shc-interacting NPxY motif in the cytoplasmic juxtamembrane region (5). Two additional kinase deficient splice forms are expressed in colon cancer (6). The discoidin-like domain mediates binding to collagens I - V (1, 7, 8). DDR1 selectively recognizes the triple helical structure of collagen (7, 8). It is expressed on the cell surface as a dimer which can include different isoforms (5, 9). DDR1 oligomerization enhances collagen binding and also modulates collagen fibrillogenesis (10, 11). The transmembrane segment contains a leucine zipper and GxxxG motif, but neither is exclusively required for dimerization (9). Collagen binding induces prolonged autophosphorylation, including the NPxY motif (7, 8). Collagen binding also results in the proteolytic cleavage of a tyrosine phosphorylated 60 kDa C-terminal fragment (CTF), and a 60 kDa ECD fragment (12, 13). TIMP3 and TAPI-1 inhibit shedding of the ECD fragment but not the CTF (12). Overexpression of DDR1a promotes MMP-2 activation and results in an increased invasiveness of a glioblastoma cell line; DDR1b does not (14).
- Vogel, W.F. et al. (2006) Cell. Signal. 18:1108.
- Yoshimura, T. et al. (2005) Immunologic Res. 31:219.
- Perez, J.L. et al. (1994) Oncogene 9:211.
- Laval, S. et al. (1994) Cell Growth Differ. 5:1173.
- Perez, J.L. et al. (1996) Oncogene 12:1469.
- Alves, F. et al. (2001) FASEB J. 15:1321.
- Shrivastava, A. et al. (1997) Mol. Cell 1:25.
- Vogel, W. et al. (1997) Mol. Cell 1:13.
- Nordeen, N.A. et al. (2006) J. Biol. Chem. 281:22744.
- Leitinger, B. (2003) J. Biol. Chem. 278:16761.
- Agarwal, G. et al. (2007) J. Mol. Biol. 367:443.
- Slack, B.E. et al. (2006) J. Cell Biochem. 98:672.
- Vogel, W.F. et al. (2001) FEBS Lett. 514:175.
- Ram, R. et al. (2006) J. Neurooncol. 76:239.
Citations for Recombinant Human DDR1 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Matrix stiffness exacerbates the proinflammatory responses of vascular smooth muscle cell through the DDR1-DNMT1 mechanotransduction axis
Authors: J Wang, SA Xie, N Li, T Zhang, W Yao, H Zhao, W Pang, L Han, J Liu, J Zhou
Bioactive materials, 2022;17(0):406-424.
Sample Types: Peptides
Selective pharmacological inhibition of DDR1 prevents experimentally-induced glomerulonephritis in prevention and therapeutic regime
Authors: S Moll, Y Yasui, A Abed, T Murata, H Shimada, A Maeda, N Fukushima, M Kanamori, S Uhles, L Badi, T Cagarelli, I Formentini, F Drawnel, G Georges, T Bergauer, R Gasser, RD Bonfil, R Fridman, H Richter, J Funk, MJ Moeller, C Chatzianto, M Prunotto
J Transl Med, 2018;16(1):148.
Sample Types: Recombinant Protein
Applications: Antibody Production
Collagen signaling enhances tumor progression after anti-VEGF therapy in a murine model of pancreatic ductal adenocarcinoma.
Authors: Aguilera K, Rivera L, Hur H, Carbon J, Toombs J, Goldstein C, Dellinger M, Castrillon D, Brekken R
Cancer Res, 2014;74(4):1032-44.
Sample Types: Protein
Applications: ELISA Developmet
Implication of discoidin domain receptor 1 in T cell migration in three-dimensional collagen.
Authors: Hachehouche LN, Chetoui N, Aoudjit F
Mol. Immunol., 2010;47(9):1866-9.
Sample Types: Whole Cells
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