Recombinant Human Desert Hedgehog/Dhh (C23II) N-Terminus, CF

Catalog # Availability Size / Price Qty
4777-DH-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (3)
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Recombinant Human Desert Hedgehog/Dhh (C23II) N-Terminus, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is 15-45 µg/mL.
Source
E. coli-derived human Desert Hedgehog/Dhh protein
Cys23-Gly198 (Cys23Ile-Ile), with and without an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met & Ile-Ile
Predicted Molecular Mass
20 kDa

Product Datasheets

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4777-DH

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4777-DH

Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and DTT with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Desert Hedgehog/Dhh

Desert Hedgehog (Dhh) belongs to the highly conserved Hedgehog family of proteins which are involved in multiple developmental processes. Desert Hedgehog is a secreted, 45 kDa, 373 amino acid (aa) protein that undergoes autocatalytic cleavage between Gly198 and Cys199 catalyzed by the C-terminal domain, which releases the N-terminal domain with a concominant attachment of cholesterol at its new C-terminus. In addition to the C-terminally attached cholesterol, a fatty acid acyl chain is esterified to the N-terminal cysteine (aa 23) via an amide linkage. The 19 kDa N-terminal signaling domain is membrane associated due to its double lipid modifications. Its binding to Patched receptors results in the loss of Patched repression of Smoothened signaling (1 - 4). Dhh binds both Patched and Patched 2 as well as Hedgehog interacting protein (Hip) (5). Within the N-terminal domain, human Dhh shares 97% aa sequence identity with mouse and rat Dhh. It shares approximately 75% aa seqeuence identity with human Indian (Ihh) and Sonic Hedgehog (Shh) (5). Dhh is produced by Sertoli cells and is required for testis development and spermatogenesis (6, 7). It induces steroidogenic factor 1, which is instrumental in promoting Leydig cell differentiation (8, 9). It also promotes the deposition of basal lamina surrounding seminiferous tubules (6). Mutations of Dhh are linked to complete pure gonadal dysgenesis (10). Dhh is expressed in the female by ovarian granulosa cells and the corporus luteum (11). Its upregulation in ovarian cancer correlates positively with proliferative index, and negatively with prognosis (12). Dhh is also expressed by Schwann cells and is upregulated following nerve injury (13, 14). It induces the expression of Patched and Hip in neural tissue fibroblasts and promotes the formation of the connective tissue sheath surrounding peripheral nerves (13).

References
  1. van den Brink, G.R. (2007) Physiol. Rev. 87:1343. 
  2. Riobo, N.A. and D.R. Manning (2007) Biochem. J. 403:369. 
  3. Porter, J.A. et al. (1995) Nature 374:363. 
  4. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. 95:13630. 
  5. Pathi, S. et al. (2001) Mech. Dev. 106:107. 
  6. Pierucci-Alves, F. et al. (2001) Biol. Reprod. 65:1392. 
  7. Bitgood, M.J. et al. (1996) Curr. Biol. 6:298. 
  8. Yao, H.H-C. et al. (2002) Genes Dev. 16:1433.
  9. Park, S.Y. et al. (2007) Endocrinology 148:3704.
  10. Canto, P. et al. (2004) J. Clin. Endocrinol. Metab. 89:4480.
  11. Russell, M.C. et al. (2007) Biol. Reprod. 77:226.
  12. Chen, X. et al. (2007) Cancer Sci. 98:68.
  13. Parmantier, E. et al. (1999) Neuron 23:713.
  14. Bajestan, S.N. et al. (2006) J. Neurobiol. 66:243.
Entrez Gene IDs
50846 (Human); 13363 (Mouse)
Alternate Names
desert hedgehog (Drosophila) homolog; desert hedgehog homolog; desert hedgehog protein; Desert Hedgehog; DHH; HHG-3MGC35145

Citations for Recombinant Human Desert Hedgehog/Dhh (C23II) N-Terminus, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. SARS-CoV-2 Enters Human Leydig Cells and Affects Testosterone Production In Vitro
    Authors: Li, L;Sottas, CM;Chen, HY;Li, Y;Cui, H;Villano, JS;Mankowski, JL;Cannon, PM;Papadopoulos, V;
    Cells
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Chondrocyte proliferation regulated by secreted luminal domain of ER stress transducer BBF2H7/CREB3L2.
    Authors: Saito A, Kanemoto S, Zhang Y, Asada R, Hino K, Imaizumi K
    Mol Cell, 2013-12-12;53(1):127-39.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  3. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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