Recombinant Human Desmoglein-2 Fc Chimera Protein, CF
Recombinant Human Desmoglein-2 Fc Chimera Protein, CF Summary
Accession # CAA81226
|IEGRMD||Human IgG1 |
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Desmoglein-2 (DSG2) is a 160 kDa transmembrane glycoprotein in the cadherin family of calcium dependent adhesion molecules. It is a major protein component of desmosomal contacts between epithelial cells (1, 2). Mature human Desmoglein-2 consists of a 560 amino acid (aa) extracellular domain (ECD) with four cadherin domains, a 25 aa transmembrane segment, and a 484 aa cytoplasmic domain with six desmoglein repeats (3). Within the ECD, human Desmoglein-2 shares 81% aa sequence identity with mouse and rat Desmoglein-2. A 60 kDa ECD fragment can be proteolytically shed, and this is enhanced by EGF R activation (4‑6). The cytoplasmic domain is cleaved during apoptosis, leaving the transmembrane segment and ECD as a cell-associated fragment (7). Desmoglein-2 is widely expressed in epithelia and is required for maintenance of the epithelial barrier (1, 2, 8). It can be either up- or down-regulated in a variety of carcinomas during tumor progression (9). Desmoglein-2 also functions as a cell adhesion receptor for several adenovirus serotypes (10). This binding disrupts intercellular junctions between epithelial cells and promotes epithelial-mesenchymal transition (EMT) (10). Mutations in Desmoglein-2 cause cardiac dysfunction typical of arrythmogenic right ventricular cardiomyopathy (ARVC) (11).
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- Schlegel, N. et al. (2010) Am. J. Physiol. Gastrointest. Liver Physiol. 298:G774.
- Dusek, R.L. and L.D. Attardi (2011) Nat. Rev. Cancer 11:317.
- Wang, H. et al. (2011) Nat. Med. 17:96.
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Citation for Recombinant Human Desmoglein-2 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Diversity within the adenovirus fiber knob hypervariable loops influences primary receptor interactions
Authors: AT Baker, A Greenshiel, L Coughlan, JA Davies, H Uusi-Kertt, DK Cole, PJ Rizkallah, AL Parker
Nat Commun, 2019-02-14;10(1):741.
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance
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