Recombinant Human Desmoglein-3 His-tag Protein, CF

Catalog # Availability Size / Price Qty
11167-DM-050

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Recombinant Human Desmoglein‑3 His-tag Protein Bioactivity.
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Recombinant Human Desmoglein-3 His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of immobilized Recombinant Human Desmoglein‑3 His-tag (Catalog # 11167-DM)  to support the adhesion of Caki‑2 human clear cell carcinoma epithelial cells. The ED50 for this effect is 0.750-6.00 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Desmoglein-3 protein
Glu24-Lys499, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Glu24 & Glu50
Predicted Molecular Mass
56 kDa & 53 kDa
SDS-PAGE
57-67 kDa, under reducing conditions.

Product Datasheets

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11167-DM

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11167-DM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Human Desmoglein‑3 His-tag Protein Bioactivity. View Larger

Measured by the ability of immobilized Recombinant Human Desmoglein‑3 His-tag Protein (Catalog # 11167-DM) to support the adhesion of Caki‑2 human clear cell carcinoma epithelial cells. The ED50 for this effect is 0.750-6.00 μg/mL.

SDS-PAGE Recombinant Human Desmoglein‑3 His-tag Protein SDS-PAGE. View Larger

2 μg/lane of Recombinant Human Desmoglein‑3 His-tag Protein (Catalog # 11167-DM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 57-67 kDa.

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Background: Desmoglein-3

Desmoglein-3, also known as pemphigus vulgaris antigen (PVA), is a 130 kDa transmembrane glycoprotein that belongs to the cadherin family of calcium dependent adhesion molecules (1 - 3). Human Desmoglein-3 is synthesized with a 26 amino acid (aa) propeptide. The mature protein consists of a 566 aa extracellular domain (ECD) that contains four cadherin-like domains, a 25 aa transmembrane segment, and a 359 aa cytoplasmic domain (4). Within the ECD, human Desmoglein-3 shares 78% aa sequence identity with mouse and rat Desmoglein-3. It shares 44%, 47%, and 56% aa sequence identity with Desmoglein-1, -2, and -4 respectively. Desmoglein-3 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells (1, 2). It is expressed in the basal and suprabasal layers of stratified epithelia in many tissues (4 - 6). During apoptosis, Desmoglein-3 is cleaved by caspases, plus MMP-2, and MMP-9 at sites within the cytoplasmic and extracellular regions, resulting in shortened transmembrane forms and a soluble 75 kDa ECD fragment (7, 8). The downregulation of Desmoglein-3 in oral squamous cell carcinoma correlates with metastatic potential (9). Desmoglein-3 is the target of autoantibodies in pemphigus vulgaris, a blistering skin disorder with compromised inter-keratinocyte adhesion (6, 10). Binding of these antibodies triggers Desmoglein-3 internalization and degradation by keratinocytes (10).

References
  1. Kottke, M.D. et al. (2006) J. Cell Sci. 119:797.
  2. Garrod, D.R. et al. (2002) Mol. Membr. Biol. 19:81.
  3. Leckband, D. and A. Prakasam (2006) Annu. Rev. Biomed. Eng. 8:259.
  4. Amagai, M. et al. (1991) Cell 67:869.
  5. Silos, S.A. et al. (1996) J. Biol. Chem. 271:17504.
  6. Amagai, M. et al (1996) J. Invest. Dermatol. 106:351.
  7. Weiske, J. et al. (2001) J. Biol. Chem. 276:41175.
  8. Cirillo, N. et al. (2007) Oral Dis. 13:341.
  9. Wang, L. et al. (2007) Virchows Arch. Sept. 11 epub.
  10. Calkins, C.C. et al. (2006) J. Biol. Chem. 281:7623.
Entrez Gene IDs
1830 (Human); 13512 (Mouse)
Alternate Names
Cadherin family member 6; CDHF6; CDHF6130-kD pemphigus vulgaris antigen; desmoglein 3 (pemphigus vulgaris antigen); desmoglein 3; Desmoglein3; Desmoglein-3; DKFZp686P23184,130 kDa pemphigus vulgaris antigen; DSG3; pemphigus vulgaris antigen; PVA

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