Recombinant Human DLL4 Fc Chimera GMP Protein, CF

Key Product Details

Product Specifications

Scientific Data Images for Recombinant Human DLL4 Fc Chimera GMP Protein, CF

Formulation, Preparation, and Storage

Calculators

Background: DLL4

Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. DLL-4 is the only Notch ligand expressed predominantly by the vascular endothelium,  making it critical in vascular development.  The mature extracellular domain (ECD) of human DLL4 shares 86% amino acid sequence identity with mouse DLL4.  The use of DLL4, in conjunction with VCAM1, has been shown as an effective method for generating hematopoietic progenitors and T cells from hPSCs in a serum- and feeder-free environment.

Additional DLL4 Products

• All Products for DLL4
• DLL4 cDNA Clones
• DLL4 ELISA Kits
• DLL4 Primary Antibodies
• DLL4 Proteins and Enzymes

Product Documents for Recombinant Human DLL4 Fc Chimera GMP Protein, CF

Manufacturing Specifications

GMP Proteins
R&D Systems, a Bio-Techne Brand's GMP proteins are produced according to relevant sections of the following documents: USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Eu. Ph. 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products.

R&D Systems' quality focus includes:

• Designed, manufactured and tested under an ISO 9001:2015 and ISO 13485:2016 certified quality system
• Documented and controlled manufacturing process
• Control of documentation and process changes by QA
• Personnel training programs
• Raw material inspection and vendor qualification/monitoring program
• Validated equipment, processes and test methods
• Equipment calibration and maintenance schedules using a Regulatory Asset Manager
• Facility/Utilities maintenance, contamination controls, safety and pest control programs
• Material review process for variances
• Robust product stability program following relevant ICH guidelines
  
  

R&D Systems strives to provide our customers with the analytical characteristics of each product so that customers may determine whether our products are appropriate for their application. Each product is provided with a lot-specific Certificate of Analysis that contains the product's specifications and test results. Quality control testing may include, but is not limited to:

• N-terminal amino acid analysis
• SDS-PAGE purity analysis
• Molecular weight analysis via mass spectrometry
• Endotoxin assessment per USP <85> and Ph. Eur. 2.6.14 guidelines
• Bioassay analysis
• Microbial testing per USP <71> and Ph. Eur. 2.6.1 guidelines
• Host cell protein assessment
• Host cell DNA assessment
• Mycoplasma assessment
  
  

Additional testing and documentation requested by the customer can be arranged at an additional cost. 

Production records and facilities are available for examination by appropriate personnel on-site at R&D Systems in Minneapolis and St. Paul, Minnesota USA.

R&D Systems sells GMP grade products for preclinical or clinical ex vivo use. They are not for in vivo use. Please read the following End User Terms prior to using this product.