Recombinant Human EG-VEGF/PK1 Protein

Carrier Free

Catalog # Availability Size / Price Qty
1209-EV-025/CF

With Carrier

Catalog # Availability Size / Price Qty
1209-EV-025
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human EG-VEGF/PK1 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using EJG bovine adrenal-derived endothelial cells. The ED50 for this effect is 1-4 µg/mL. Measured in a cell proliferation assay using human pancreatic cancer cells. The ED50 for this effect is 1-4 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human EG-VEGF/PK1 protein
Ala20-Phe105, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ala20
Predicted Molecular Mass
10.5 kDa

Product Datasheets

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1209-EV (with carrier)

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1209-EV/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1209-EV

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1209-EV/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EG-VEGF/PK1

Endocrine gland-derived vascular endothelial growth factor (EG-VEGF), also called prokineticin 1 (PK1), is a member of the prokineticin family of secreted proteins that share a common structural motif containing ten conserved cysteine residues that form five pairs of disulfide bonds (1, 2). Members of this family include the mammalian EG-VEGF/PK1 and PK2, as well as the venom protein A (VPRA) from the venom of black mamba snake and the frog Bombina variegata, Bv8 (1). Human EG-VEGF precursor is a 105 amino acid (aa) residue protein with a 19 aa signal peptide that is cleaved to yield a 86 aa mature protein (1, 2). EG-VEGF is expressed in multiple tissues including the gastrointestinal (GI) tract and steroidogenic glands (testis, ovary, placenta and adrenal glands). EG-VEGF has been shown to potently stimulate the contraction of GI smooth muscle. In addition, EG-VEGF is a tissue-specific angiogenic factor that exhibits biological activities similar to that of VEGF on select cells. It induces the proliferation, migration, and fenestration in cultured endocrine gland-derived capillary endothelial cells. EG-VEGF binds to and activates two closely related G protein-coupled receptors, EG‑VEGF/PK1-R1 and EG-VEGF/PK2-R2 (3, 4). Activation of the receptors leads to stimulation of phosphoinositide turnover and activation of p44/p42 MAP kinase signaling pathways.

References
  1. Li, M. et al. (2001) Mol. Pharmacol. 59:692.
  2. LeCouter, J. et al. (2001) Nature 412:877.
  3. Lin, D. et al. (2002) J. Biol. Chem. 277:19276.
  4. Masuda, Y. et al. (2002) Biochem. Biophys. Res. Commun. 293:396.
Long Name
Endocrine Gland-derived Vascular Endothelial Growth Factor
Entrez Gene IDs
84432 (Human); 246691 (Mouse); 192205 (Rat)
Alternate Names
EGVEGF; EG-VEGF; EGVEGFblack mamba toxin-related protein; Endocrine-gland-derived vascular endothelial growth factor; Mambakine; PK1mambakine; PRK1prokineticin-1; PROK1; Prokineticin 1

Citation for Recombinant Human EG-VEGF/PK1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Diversity in human placental microvascular endothelial cells and macrovascular endothelial cells
    Authors: X Huang, L Jia, Z Qian, Y Jia, X Chen, X Xu, X Chang, M Liu, K Wang
    Cytokine, 2018;111(0):287-294.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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