Recombinant Human Elongin B/Elongin C/VHL Complex, CF

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R&D Systems Recombinant Proteins and Enzymes
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Citations (2)

Recombinant Human Elongin B/Elongin C/VHL Complex, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Typical protein concentration for use in vitro will depend on experimental conditions.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human ELOB/ELOC/VHL Complex protein
Met1 - Gln118 (ELOB), Met1 - Cys112 (ELOC), Met231 - Asp443 (VHL)
Predicted Molecular Mass
24 kDa (VHL), 13.1 kDa (ELOB), 12.8 kDa (ELOC)

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Background: ELOB/ELOC/VHL Complex

Together, Elongin B (ELOB) and Elongin C (ELOC) form a heterodimer that serves as the regulatory subunit for the Elongin complex--a general transcription elongation factor that increases RNA Polymerase II transcription through template-encoded arresting sites.  The ELOB/ELOC complex also binds to the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families.  In this function, ELOB/ELOC serves as an adapter between substrate recognition proteins and either Cullin-2/Rbx1 (in VHL-box E3 Ubiquitin ligases) or Cullin-5/Rbx2 (in SOCS-box E3 Ubiquitin ligases).  VHL (von Hippel-Lindau disease tumor suppressor) is the substrate recognition subunit for an E3 ligase activity that ubiquitinates proteins containing hydroxyproline residues.  Targets of VHL include HIF1 alpha, beta 2 adrenergic receptor, ZHX2 and others.  

  1. Kamura T., et al.  (1998) Genes Dev. 12:3872.
  2. Okumura F., et al.  (2012) Front. Oncol. 2:10.
  3. Xie L., et al.  (2009) Sci. Signal. 2:ra33.
  4. Zhang J. et al.  (2018) Science 361:290. 
Long Name
Elongin B/Elongin C/VHL Complex
Alternate Names
ELOB/ELOC/VHL Complex; Elongin B/Elongin C/VHL Complex

Citations for Recombinant Human Elongin B/Elongin C/VHL Complex, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Rational screening for cooperativity in small-molecule inducers of protein-protein associations
    Authors: Liu, S;Tong, B;Mason, JW;Ostrem, JM;Tutter, A;Hua, BK;Tang, SA;Bonazzi, S;Briner, K;Berst, F;Zécri, FJ;Schreiber, SL;
    bioRxiv : the preprint server for biology
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Bridged Proteolysis Targeting Chimera (PROTAC) Enables Degradation of Undruggable Targets
    Authors: Y Xiong, Y Zhong, H Yim, X Yang, KS Park, L Xie, PI Poulikakos, X Han, Y Xiong, X Chen, J Liu, J Jin
    Journal of the American Chemical Society, 2022-11-30;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Pull-Down Assay


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