Recombinant Human Elongin B/Elongin C/VHL Complex, CF
Recombinant Human Elongin B/Elongin C/VHL Complex, CF Summary
Product Specifications
Met1 - Gln118 (ELOB), Met1 - Cys112 (ELOC), Met1 - Asp213 (VHL)
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E3-600
| Formulation | Supplied as a solution in HEPES, NaCl, DTT and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: ELOB/ELOC/VHL Complex
Together, Elongin B (ELOB) and Elongin C (ELOC) form a heterodimer that serves as the regulatory subunit for the Elongin complex--a general transcription elongation factor that increases RNA Polymerase II transcription through template-encoded arresting sites. The ELOB/ELOC complex also binds to the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families. In this function, ELOB/ELOC serves as an adapter between substrate recognition proteins and either Cullin-2/Rbx1 (in VHL-box E3 Ubiquitin ligases) or Cullin-5/Rbx2 (in SOCS-box E3 Ubiquitin ligases). VHL (von Hippel-Lindau disease tumor suppressor) is the substrate recognition subunit for an E3 ligase activity that ubiquitinates proteins containing hydroxyproline residues. Targets of VHL include HIF1 alpha, beta 2 adrenergic receptor, ZHX2 and others.
Citations for Recombinant Human Elongin B/Elongin C/VHL Complex, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Rational screening for cooperativity in small-molecule inducers of protein-protein associations
Authors: Liu, S;Tong, B;Mason, JW;Ostrem, JM;Tutter, A;Hua, BK;Tang, SA;Bonazzi, S;Briner, K;Berst, F;Zécri, FJ;Schreiber, SL;
bioRxiv : the preprint server for biology
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay -
Bridged Proteolysis Targeting Chimera (PROTAC) Enables Degradation of Undruggable Targets
Authors: Y Xiong, Y Zhong, H Yim, X Yang, KS Park, L Xie, PI Poulikakos, X Han, Y Xiong, X Chen, J Liu, J Jin
Journal of the American Chemical Society, 2022-11-30;0(0):.
Species: Human
Sample Types: Protein
Applications: Pull-Down Assay
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