Recombinant Human Elongin B/Elongin C/VHL Complex, CF

Catalog # Availability Size / Price Qty
E3-600-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human Elongin B/Elongin C/VHL Complex, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Typical protein concentration for use in vitro will depend on experimental conditions.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human ELOB/ELOC/VHL Complex protein
Predicted Molecular Mass
24 kDa (VHL), 13.1 kDa (ELOB), 12.8 kDa (ELOC)

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-600

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 2 mM DTT
Shipping
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 24 months from date of receipt, -70 °C as supplied.
  • 6 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ELOB/ELOC/VHL Complex

Together, Elongin B (ELOB) and Elongin C (ELOC) form a heterodimer that serves as the regulatory subunit for the Elongin complex--a general transcription elongation factor that increases RNA Polymerase II transcription through template-encoded arresting sites.  The ELOB/ELOC complex also binds to the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families.  In this function, ELOB/ELOC serves as an adapter between substrate recognition proteins and either Cullin-2/Rbx1 (in VHL-box E3 Ubiquitin ligases) or Cullin-5/Rbx2 (in SOCS-box E3 Ubiquitin ligases).  VHL (von Hippel-Lindau disease tumor suppressor) is the substrate recognition subunit for an E3 ligase activity that ubiquitinates proteins containing hydroxyproline residues.  Targets of VHL include HIF1 alpha, beta 2 adrenergic receptor, ZHX2 and others.  This protein complex is untagged.

References
  1. Kamura T., et al.  (1998) Genes & Dev.  doi:10.1101/gad.12.24.3872
  2. Okumura F., et al.  (2012) Front. Oncol.  doi:10.3389/fonc.2012.00010
  3. Xie L., et al.  (2009) Sci. Signaling  doi:10.1126/scisignal.2000444
  4. Zhang J. et al.  (2018) Science  doi:10.1126/science.aap8411
Long Name
Elongin B/Elongin C/VHL Complex
Alternate Names
ELOB/ELOC/VHL Complex; Elongin B/Elongin C/VHL Complex

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