Recombinant Human EphA10 Fc Chimera Protein, CF Summary
Accession # NP_001092909
|IEGRMD||Human IgG1 |
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 200 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
EphA10 is a member of the transmembrane Eph receptor tyrosine kinase family. Although the A and B classes of Eph proteins have a common structural organization, they are distinguished by their preference for Ephrin ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1-4). The 532 amino acid (aa) extracellular domain (ECD) of human EphA10 contains an N-terminal Ephrin binding region followed by two fibronectin type II domains, a 422 aa cytoplasmic domain contains the tyrosine kinase domain and a sterile alpha motif (SAM) (5). Alternate splicing generates a soluble isoform that is truncated following the Ephrin binding region, and a transmembrane isoform that lacks only the SAM (5). Within the ECD, human EphA10 shares 38%-54% aa sequence identity with other EphA and EphB proteins. It shares 95% and 88% aa sequence identity with the ECD of mouse and rat EphA10, respectively. EphA10 is preferentially expressed in testis and is upregulated in some breast and prostate carcinoma cell lines (5- 7). It selectivity binds Ephrin-A1, 2, 3, 4,and 5 relative to Ephrin-B1 and -B2 (5).
- Pasquale, E.B. (2008) Cell 133:38.
- Merlos-Suarez, A. and E. Batlle (2008) Curr. Opin. Cell Biol. 20:194.
- Arvanitis, D. and A. Davy (2008) Genes Dev. 22:416.
- Robinson, D.R. et al. (2000) Oncogene 19:5548.
- Aasheim, H.-C. et al. (2005) Biochim. Biophys. Acta 1723:1.
- Fox, B.P. et al. (2006) Biochem. Biophys. Res. Commun. 342:1263.
- Fox, B.P. and R.P. Kandpal (2004) Biochem. Biophys. Res. Commun. 318:882.
Citation for Recombinant Human EphA10 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancer
Authors: JH Cha, LC Chan, YN Wang, YY Chu, CH Wang, HH Lee, W Xia, WC Shyu, SP Liu, J Yao, CW Chang, FR Cheng, J Liu, SO Lim, JL Hsu, WH Yang, GN Hortobagyi, C Lin, L Yang, D Yu, LB Jeng, MC Hung
The Journal of Biological Chemistry, 2022-03-10;0(0):101817.
Species: Human, N/A
Sample Types: Protein, Whole Cells
Applications: Bioassay, ELISA Capture
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