Recombinant Human EphA8 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
6828-A8-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human EphA8 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human EphA8 Fc Chimera is coated at 2 μg/mL (100 μL/well), the concentration of Biotinylayed Recombinant Human Ephrin-A5 Fc Chimera (Catalog # BT374) that produces 50% of the optimal binding response is 2-12 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human EphA8 protein
Human EphA8
(Glu31-Thr542)
Accession # NP_065387
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Glu31
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
83.2 kDa (monomer)
SDS-PAGE
94 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6828-A8

Formulation Supplied as a 0.2 μm filtered solution in MES, NaCl, PEG, CHAPS and Imidazole.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

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Background: EphA8

EphA8, also known as Hek3 and Eek, is a 120 kDa glycosylated member of the Eph family of transmembrane receptor tyrosine kinases (1, 2). The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. EphA4 binds and is activated by class A Ephrins but not class B Ephrins (3, 4). Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. The 512 amino acid (aa) extracellular domain (ECD) of human EphA8 contains an N-terminal Ephrin binding region, a cysteine-rich region, and two fibronectin type III domains (FnIII). The 442 aa cytoplasmic domain contains the tyrosine kinase domain and a sterile alpha motif (SAM) (5). Within the ECD, human EphA8 shares 97% aa sequence identity with mouse and rat EphA8. EphA8 is expressed in neurons in the mesencephalon of the developing brain, particularly in the rostral tectum and the superior colliculus (6-8). It is enriched at the tips of neurite processes and plays a role in projection of superior colliculus axons through the posterior commissure (7, 9). Its expression enhances neurite extension by means of a mechanism that does not require catalytic activity of the tyrosine kinase domain (10). Ephrin-mediated activation of the EphA8 kinase induces phosphorylation of tyrosine residues in the cytoplasmic domain, leading to association with signaling and scaffolding proteins and inhibition of cell-cell adhesion (4, 9, 11). Ligand binding can also promote the Integrin-mediated cellular adhesion to Fibronectin (12). This function, like the enhancement of neurite extension, does not require activation of the kinase domain (12).

References
  1. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
  2. Merlos-Suarez, A. and E. Batlle (2008) Curr. Opin. Cell Biol. 20:194.
  3. Park, S. and M.P. Sanchez (1997) Oncogene 14:533.
  4. Choi, S. et al. (1999) Mol. Cells 9:440.
  5. Chan, J. and V.M. Watt (1991) Oncogene 6:1057.
  6. Koo, J. et al. (2003) Dev. Dyn. 226:596.
  7. Park, S. et al. (1997) EMBO J. 16:3106.
  8. Shim, S. et al. (2007) Mol. Cell. Biol. 27:1614.
  9. Shin, J. et al. (2007) Mol. Cell. Biol. 27:8113.
  10. Gu, C. et al. (2005) Oncogene 24:4243.
  11. Choi, S. and S. Park (1999) Oncogene 18:5413.
  12. Gu, C. and S. Park (2001) Mol. Cell. Biol. 21:4579.
Long Name
Eph Receptor A8
Entrez Gene IDs
2046 (Human); 13842 (Mouse)
Alternate Names
Eek; EK3; EPH- and ELK-related kinase; EPH- and ELK-related tyrosine kinase; EPH receptor A8; EphA8; EPH-like kinase 3; ephrin type-A receptor 8; Hek3; hydroxyaryl-protein kinase; KIAA1459; protein-tyrosine kinase; tyrosine-protein kinase receptor EEK; tyrosylprotein kinase

Citation for Recombinant Human EphA8 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancer
    Authors: JH Cha, LC Chan, YN Wang, YY Chu, CH Wang, HH Lee, W Xia, WC Shyu, SP Liu, J Yao, CW Chang, FR Cheng, J Liu, SO Lim, JL Hsu, WH Yang, GN Hortobagyi, C Lin, L Yang, D Yu, LB Jeng, MC Hung
    The Journal of Biological Chemistry, 2022;0(0):101817.
    Species: N/A
    Sample Types: Protein
    Applications: ELISA Capture

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