Recombinant Human EphB3 Fc Chimera Protein, CF

R&D Systems | Catalog # 5667-B3

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human EphB3 Fc Chimera Protein (5667-B3)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P54753

Structure / Form

Disulfide-linked homodimer

Applications

Binding Activity
View all EphB3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human EphB3 protein
Leu38-Ala550

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu38

Predicted Molecular Mass

82.7 kDa (monomer)

SDS-PAGE

90-100 kDa under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Human EphB3 Fc Chimera at 2 μg/mL (100 μL/well) can bind recombinant mouse Ephrin-B1 Fc Chimera with a linear range of 0.04-2.5 ng/mL

Reviewed Applications

Read 1 review rated 4 using 5667-B3 in the following applications:

Formulation, Preparation, and Storage

5667-B3
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EphB3

EphB3, also known as Cek10, Tyro6, Sek4, Hek2, and Mdk5, is a 130 kDa member of the transmembrane Eph receptor tyrosine kinase family. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1). The 526 amino acid (aa) extracellular domain (ECD) of mature human EphB3 contains a ligand binding domain followed by a cysteine rich region and two fibronectin type III domains. The 418 aa cytoplasmic domain contains a tyrosine kinase domain, a sterile alpha motif (SAM), and a PDZ binding motif (2). Within the ECD, human EphB3 shares 96% aa sequence identity with mouse and rat EphB3. Binding of EphB3 to its ligands Ephrin-B1, B2, and B3 triggers forward signaling through EphB3 as well as reverse signaling through the Ephrin (1, 3). EphB3 also interacts in cis with the receptor tyrosine kinase Ryk (4). Activation of its kinase is required for some but not all of the effects of EphB3 on cellular adhesion, motility, and morphology (5). EphB3 is widely expressed during development and in the adult; it shows a complementary tissue distribution to the Ephrin-B ligands (6-9). EphB3 function is important in vascular, nervous system, thymocyte, and palate development (6, 7, 10-12). It directs embyronic neuronal axon pathfinding, and its up-regulation on local macrophages following neuronal injury promotes the growth of regenerating axons (10, 13). EphB3 inhibits colorectal carcinogenesis and invasion by preventing the migration of tumor cells out of the intestinal crypt (9, 14). In non-small cell lung cancer, however, it is up-regulated and can promote tumor progression (15). EphB3 function is supported by the cooperative action of EphB2 in several of these processes (6, 10-12, 16).

References

  1. Park, I. and H.S. Lee (2015) Mol. Cells 38:14.
  2. Bohme, B. et al. (1993) Oncogene 8:2857.
  3. Pasquale, E.B (2004) Nat. Neurosci. 7:417.
  4. Trivier, E. and T.S. Ganesan (2002) J. Biol. Chem. 277:23037.
  5. Miao, H. et al. (2005) J. Biol. Chem. 280:923.
  6. Adams, R.H. et al. (1999) Genes Dev. 13:295.
  7. Krull, C.E. et al. (1997) Curr. Biol. 7:571.
  8. Willson, C.A. et al. (2006) J. Mol. Histol. 37:369.
  9. Cortina, C. et al. (2007) Nature Genet. 39:1376.
  10. Birgbauer, E. et al. (2000) Development 127:1231.
  11. Alfaro, D. et al. (2008) Immunology 125:131.
  12. Risley, M. et al. (2009) Mech. Dev. 126:230.
  13. Liu, X. et al. (2006) J. Neurosci. 26:3087.
  14. Batlle, E. et al. (2005) Nature 435:1126.
  15. Ji, X.-D. et al. (2011) Cancer Res. 71:1156.
  16. Holmberg, J. et al. (2006) Cell 125:1151.

Long Name

Eph Receptor B3

Alternate Names

Cek10, Hek2, Mdk5, Sek4, Tyro6

Entrez Gene IDs

2049 (Human); 13845 (Mouse)

Gene Symbol

EPHB3

UniProt

P54753 (Human)

Additional EphB3 Products

Product Documents for Recombinant Human EphB3 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human EphB3 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human EphB3 Fc Chimera Protein, CF

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  • Recombinant Human EphB3 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 09/23/2019
    We used this protein as a standard for developing an ELISA in combination with mAb (cat. MAB5667) and pAb (cat. AF5667). This combination works well detecting Ephb3 in human serum.
    Recombinant Human EphB3 Fc Chimera Protein, CF 5667-B3

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