Recombinant Human Ephrin-B3 Fc Chimera Protein, CF

R&D Systems | Catalog # 7924-EB

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human Ephrin-B3 Fc Chimera Protein (7924-EB)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

NP_001397

Structure / Form

Disulfide-linker homodimer

Applications

Bioactivity
View all Ephrin-B3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Ephrin-B3 protein
Human Ephrin-B3
(Leu28-Ser224)
Accession # NP_001397		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu28

Predicted Molecular Mass

48.3 kDa (monomer)

SDS-PAGE

57-61 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human EphB3 Fc Chimera (Catalog # 432-B3) is coated at 2 μg/mL, Recombinant Human Ephrin-B3 Fc Chimera binds with an apparent Kd < 0.4 nM.

Formulation, Preparation, and Storage

7924-EB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ephrin-B3

Ephrin‑B3, also known as Elk‑L3, LERK8, Eplg8, NLERK‑2, and EFL6, is an approximately 50 kDa member of the Ephrin‑B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains (ECD) of Ephrin‑B ligands are structurally related to GPI‑anchored Ephrin‑A ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin‑B3 preferentially interacts with receptors in the EphB family and also with EphA4. The binding of Ephrin‑B3 to Eph proteins also triggers reverse signaling through Ephrin‑B3 (1, 2). Mature human Ephrin‑B3 consists of a 199 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 93 aa cytoplasmic domain (3, 4). Within the ECD, human Ephrin‑B3 shares 96% and 97% aa sequence identity with mouse and rat Ephrin‑B3, respectively. Ephrin‑B3 is expressed on oligodendrocytes and neurons in the hippocampus and along the midline of the spinal cord (5‑9). It is up‑regulated in glioma and promotes tumor cell invasion and migration (10). Ephrin‑B3 functions as a repulsive axon guidance molecule by inducing growth cone collapse, neurite retraction, and axon pruning (5‑8). Its repulsive effect along the spinal cord midline restricts motor neuron axons to their ipsilateral sides, thereby maintaining the independence of voluntary left side/right side movements (8, 9). Ephrin‑B3 plays a role in the regulation of excitatory synapse density and synaptic maturation (6, 11, 12). It also functions as a cellular receptor for Nipah virus (13) and can induce the migration of memory B cells (14).

References

  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Gale, N.W. et al. (1996) Oncogene 13:1343.
  4. Nicola, N.A. et al. (1996) Growth Factors 13:141.
  5. Benson, M.D. et al. (2005) Proc. Natl. Acad. Sci. USA 102:10694.
  6. Xu, N.-J. et al. (2011) Nat. Neurosci. 14:1421.
  7. Xu, N.-J. and M. Henkemeyer (2009) Nat. Neurosci. 12:268.
  8. Kullander, K. et al. (2001) Genes Dev. 15:877.
  9. Yokoyama, N. et al. (2001) Neuron 29:85.
  10. Nakada, M. et al. (2006) Cancer Res. 66:8492.
  11. McClelland, A.C. et al. (2010) Proc. Natl. Acad. Sci. USA 107:8830.
  12. Antion, M.D. et al. (2010) Mol. Cell. Neurosci. 45:378.
  13. Negrete, O.A. et al. (2006) PLoS Pathog. 2:e7.
  14. Holen, H.L. et al. (2011) Scand. J. Immunol. 74:144.

Alternate Names

EFL-6, Efnb3, Elk-L3, EphrinB3, Epl8, LERK-8, NLERK-2

Entrez Gene IDs

1949 (Human); 13643 (Mouse)

Gene Symbol

EFNB3

UniProt

NP_001397

Additional Ephrin-B3 Products

Product Documents for Recombinant Human Ephrin-B3 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Ephrin-B3 Fc Chimera Protein, CF

For research use only

Related Research Areas

Ephrin Ligands and Eph Receptors

Citations for Recombinant Human Ephrin-B3 Fc Chimera Protein, CF

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