Recombinant Human ERMAP Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
Recombinant Human ERMAP Fc Chimera Protein Bioactivity
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Product Details

Recombinant Human ERMAP Fc Chimera Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. ED50 for this effect is 0.75-7.5 μg/mL.
Human embryonic kidney cell, HEK293-derived human ERMAP protein
Accession # Q96PL5.1
Accession #
N-terminal Sequence
His30 & Gly32
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
40 kDa
40-55 kDa, under reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity View Larger

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion by human T cells. The ED50 for this effect is 0.75-7.5 μg/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human ERMAP Fc Chimera (Catalog # 10645-ER) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-55 kDa and 80-110 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: ERMAP

Erythroid membrane-associated protein (ERMAP), also called Scianna blood group antigen, is a member of the butrophilin (BTN) and butrophilin-like (BTNL) family within the immunoglobulin superfamily. ERMAP consists of an extracellular domain (ECD) with one V-type Ig-like (IgV) fold, a transmembrane segment, and a cytoplasmic domain with a B30.2 motif (1).  Within the ECD, human ERMAP shares 33% and 31% amino acid sequence identity with mouse and rat ERMAP, respectively. Interestingly, human ERMAP contains a single IgV-like domain in the ECD, while mouse and rat ERMAP contain both an IgV-like and IgC-like domain. However, the absence of the IgC-like domain in human ERMAP does not seem to affect its functions (2, 3). ERMAP is an adhesion receptor molecule highly expressed in erythroid tissues, on the cell surface of resting and activated antigen-presenting cells (APCs) and in some tumor tissues (1, 4, 5). Fusion proteins (ERMAP-Ig) of both human and mouse ERMAP inhibit T cell functions in vitro and administration of the fusion protein ameliorates autoimmune diseases, including experimental autoimmune encephalomyelitis and type 1 diabetes, in mice (5). ERMAP acts as a novel inhibitory molecule for T cells and macrophages (5).

  1. Xu, H. et al. (2001) Genomics 76:2.
  2. Compte, E.W. et al. (2004) Eur. J. Immunol. 34:2089.
  3. Su, Y.Y. et al. (2001) Blood Cell Mol. Dis. 27:938
  4. Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
  5. Su, M. et al. (2020) Cell. Mol. Immunol. doi: 10.1038/s41423-020-0494-8.
Long Name
Erythroblast Membrane-associated Protein
Entrez Gene IDs
114625 (Human); 27028 (Mouse); 298485 (Rat)
Alternate Names
ERMAP; erythroblast membrane-associated protein (RD and SC blood groups); erythroblast membrane-associated protein (Scianna blood group); erythroblast membrane-associated protein; erythroid membrane-associated protein; hERMAP; MGC118810; MGC118811; PRO2801; Radin blood group (Rd); Radin blood group antigen; Radin blood group; RD; RDMGC118812; SC; Scianna blood group (Sc); Scianna blood group antigen; Scianna blood group; SCMGC118813


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