Recombinant Human FAM105B/OTULIN Protein, CF

Name: Recombinant Human FAM105B/OTULIN Protein, CF
Brand: R&D Systems
SKU: E-558
Rating: 5 (1 reviews)

Catalog #: E-558
2 Citations 1 Review Datasheet / COA / SDS

Discontinued Product

E-558 has been discontinued.

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Product Details

Citations (2)

FAQs

Reviews (1)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human FAM105B/OTULIN Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Activity

Recombinant Human FAM105B/OTULIN is a Linear Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human FAM105B/OTULIN concentration of 0.05-1 μM.

Source

E. coli-derived human FAM105B/OTULIN protein

Accession #

Q96BN8

Predicted Molecular Mass

40 kDa

Product Datasheets

E-558

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-558

Formulation	X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: FAM105B/OTULIN

FAM105B ("OTULIN") is a C65 type peptidase and a member of the ovarian tumor (OTU) protein super-family. OTULIN has exquisite specificity for linear (methionine-1-linked) ubiquitin linkages and has been shown to play a role in the regulation of NF-kB mediated signaling.

References

Keusekotten, K. et al. (2013) Cell 153: 1312.
Mevissen, T.E. et al. (2013) Cell 154: 169.
Radjacommare, R. et al. (2014) Front Plant Sci. 5: 84.

Long Name

Family with Sequence Similarity 105, Member B

Entrez Gene IDs

90268 (Human); 432940 (Mouse)

Alternate Names

FAM105B family with sequence similarity 105, member B; FAM105B; OTULIN

Related Research Areas

Citations for Recombinant Human FAM105B/OTULIN Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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A bio-orthogonal linear ubiquitin probe identifies STAT3 as a direct substrate of OTULIN in glioblastoma
Authors: X Du, J Pang, B Gu, T Si, Y Chang, T Li, M Wu, Z Wang, Y Wang, J Feng, N Wu, J Man, H Li, A Li, T Zhang, B Wang, X Duan
Nucleic Acids Research, 2023-02-22;0(0):.
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay
OTULIN limits cell death and inflammation by deubiquitinating LUBAC
Authors: K Heger, KE Wickliffe, A Ndoja, J Zhang, A Murthy, DL Dugger, A Maltzman, F de Sousa E, J Hung, Y Zeng, E Verschuere, DS Kirkpatric, D Vucic, WP Lee, M Roose-Girm, RJ Newman, S Warming, YC Hsiao, LG K?m?ves, JD Webster, K Newton, VM Dixit
Nature, 2018-06-27;559(7712):120-124.
Species: Mouse
Sample Types: Cell Lysates
Applications: Bioassay