Recombinant Human Fibronectin Fragment 2 Protein, CF

Catalog # Availability Size / Price Qty
3225-FN-100
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human Fibronectin Fragment 2 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells.

The ED50 for this effect is 0.25-1.25 μg/mL.

Optimal dilutions should be determined by each laboratory for each application.

Source
Mouse myeloma cell line, NS0-derived human Fibronectin protein
Ser607-Pro1265, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ser607
Predicted Molecular Mass
73 kDa
SDS-PAGE
100 kDa, reducing conditions

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3225-FN

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3225-FN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and Tween® 20.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: Fibronectin

Fibronectin, encoded by the FN1 gene, is a large modular glycoprotein that is found as a polymeric fibrillar network in the extracellular matrix (ECM) and as soluble disulfide-linked dimeric protomers in plasma and other body fluids. The protein subunit is made up of three types of homologous structural repeats termed FN type I, type II, and type III repeats. Multiple isoforms of the protein formed by alternative splicing at numerous sites, resulting in insertions of extra type III domains (EDA and ECB) or parts of the variable type III connecting segment (V/IIICS), have been identified. Fibronectin is a ligand for fibrin, heparin, chondroitin sulfate, collagen/gelatin, and many integrin receptors. It is involved in multiple cellular processes including cell adhesion/migration, blood clotting, morphogenesis, tissue repair, and cell signaling. Fibronectin functions are mediated by the insoluble polymeric fibrils in the ECM. Conversion of the non-functional soluble fibronectin to fibronectin fibrils in the ECM is tightly regulated. The process is initiated by binding of fibronectin to cell surface integrins, resulting in conformational changes and exposure of cryptic epitopes necessary for polymerization (1, 2).

The recombinant human FN1.2 contains the first 7 FN type III domains (III1 - 7). III1 binds weakly to heparin and is thought to be important for self association and fibril formation. The recombinant human FN1.2 does not contain the known tripeptide RGD domain.

References
  1. Johansson, S. et al. (1997) Frontiers in Bioscience 2:126.
  2. Geiger, B. et al. (2001) Nature Reviews Molecular Cell Biology 2:793.
Entrez Gene IDs
2335 (Human); 280794 (Bovine)
Alternate Names
CIG; ED-B; fibronectin 1; Fibronectin; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF

Product Specific Notices

Tween is a registered trademark of ICI Americas.

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